Restriction map for
pF1KE0428
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvrII BalI
BbrPI BbvCI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI BstXI
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FauI FseI HgaI HincII
HindII HpaI KpnI Ksp632I KspI
MaeIII MamI MfeI MluI MluNI
MroI MscI MunI MwoI NaeI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 360 GTATAC GT^[AC][GT]AC
AccII 556 CGCG CG^CG
AcsI 394 GAATTC [GA]^AATT[CT]
AcyI 824 GGCGCC G[GA]^CG[CT]C
AlwNI 769 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 21 CCTTAGG CC^T[ACGT]AGG
ApoI 394 GAATTC [GA]^AATT[CT]
AvaI 149 CCCGAG C^[CT]CG[GA]G
AvaII 310 GGACC G^G[AT]CC
AviII 353 TGCGCA TGC^GCA
BamHI 653 GGATCC G^GATCC
BanI 824 GGCGCC G^G[CT][GA]CC
BanII 107 GGGCTC G[GA]GC[CT]^C
BbeI 824 GGCGCC GGCGC^C
BbsI 718 GAAGAC GAAGAC(2/6)
BclI 666 TGATCA T^GATCA
BmyI 107 GGGCTC G[AGT]GC[ACT]^C
BpmI 690 CTGGAG CTGGAG(16/14)
BpuAI 718 GAAGAC GAAGAC(2/6)
BsaAI 218 CACGTA [CT]AC^GT[GA]
BsaHI 824 GGCGCC G[GA]^CG[CT]C
BsaWI 707 ACCGGA [AT]^CCGG[AT]
BseRI 259 GAGGAG GAGGAG(10/8)
BsoBI 149 CCCGAG C^[CT]CG[GA]G
Bsp1286I 107 GGGCTC G[AGT]GC[ACT]^C
BspHI 608 TCATGA T^CATGA
BspLU11I 509 ACATGT A^CATGT
Bst1107I 360 GTATAC GTA^TAC
BstUI 556 CGCG CG^CG
BstZ17I 360 GTATAC GTA^TAC
Bsu36I 21 CCTTAGG CC^T[ACGT]AGG
DraII 309 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 296 CCATGG C^C[GA][CT]GG
EaeI 677 TGGCCG [CT]^GGCC[GA]
Eco81I 21 CCTTAGG CC^T[ACGT]AGG
EcoNI 85 CCTACCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 309 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 394 GAATTC G^AATTC
FbaI 666 TGATCA T^GATCA
FspI 353 TGCGCA TGC^GCA
HaeII 824 GGCGCC [GA]GCGC^[CT]
Hin1I 824 GGCGCC G[GA]^CG[CT]C
HindIII 223 AAGCTT A^AGCTT
HphI 816 GGTGA GGTGA(8/7)
KasI 824 GGCGCC G^GCGCC
MslI 619 CATTTCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 556 CGCG CG^CG
NarI 824 GGCGCC GG^CGCC
NcoI 296 CCATGG C^CATGG
PpuMI 309 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 193 CAGCTG CAG^CTG
RcaI 608 TCATGA T^CATGA
SfoI 824 GGCGCC GGC^GCC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AfaI 507 GTAC GT^AC
771 GTAC
AflIII 217 ACACGT A^C[GA][CT]GT
509 ACATGT
Asp700I 169 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
394 GAATTCTTTC
BsaJI 90 CCAAGG C^C[ACGT][ACGT]GG
296 CCATGG
BsmAI 81 GTCTC GTCTC(1/5)
114 GTCTC
BssKI 178 CCTGG ^CC[ACGT]GG
774 CCTGG
BstNI 178 CCTGG CC^[AT]GG
774 CCTGG
BstYI 653 GGATCC [GA]^GATC[CT]
795 GGATCT
Cac8I 675 GCTGGC GC[ACGT]^[ACGT]GC
821 GCTGGC
EcoRII 178 CCTGG ^CC[AT]GG
774 CCTGG
EcoT14I 90 CCAAGG C^C[AT][AT]GG
296 CCATGG
HapII 495 CCGG C^CGG
708 CCGG
HpaII 495 CCGG C^CGG
708 CCGG
MaeII 219 ACGT A^CGT
458 ACGT
MflI 653 GGATCC [GA]^GATC[CT]
795 GGATCT
MseI 338 TTAA T^TAA
575 TTAA
MspA1I 193 CAGCTG C[AC]G^C[GT]G
680 CCGCTG
MspI 495 CCGG C^CGG
708 CCGG
MvaI 178 CCTGG CC^[AT]GG
774 CCTGG
RsaI 507 GTAC GT^AC
771 GTAC
ScrFI 178 CCTGG CC^[ACGT]GG
774 CCTGG
SfaNI 414 GATGC GCATC(5/9)
854 GCATC
StyI 90 CCAAGG C^C[AT][AT]GG
296 CCATGG
TaqI 389 TCGA T^CGA
726 TCGA
Tru9I 338 TTAA T^TAA
575 TTAA
TspRI 46 CAGTG CA[GC]TG[ACGT][ACGT]^
127 CAGTG
TthHB8I 389 TCGA T^CGA
726 TCGA
XhoII 653 GGATCC [GA]^GATC[CT]
795 GGATCT
XmnI 169 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
394 GAATTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AluI AlwI BbvI BfaI
BsiYI BslI BsmFI BsrI BstF5I
CfoI Cfr13I DdeI DpnII Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
ItaI MaeI MboI MboII MnlI
NdeII NlaIII NlaIV NspI PleI
Sau3AI Sau96I TfiI TseI Tsp509I