Restriction map for
pF1KB0946
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsiYI BslI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
FauI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI HpaII KasI KpnI
KspI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MspA1I
MspI MunI MvnI MwoI NaeI
NarI NciI NgoMIV NheI NlaIV
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 393 GTCTAC GT^[AC][GT]AC
Asp700I 1041 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 828 GAGCAC G[AT]GC[AT]^C
AvaII 99 GGTCC G^G[AT]CC
BanII 181 GAGCCC G[GA]GC[CT]^C
BbsI 705 GAAGAC GAAGAC(2/6)
BbvI 541 GCAGC GCAGC(8/12)
BclI 155 TGATCA T^GATCA
BfaI 61 CTAG C^TAG
BpuAI 705 GAAGAC GAAGAC(2/6)
BsaI 120 GGTCTC GGTCTC(1/5)
BsiHKAI 828 GAGCAC G[AT]GC[AT]^C
BsmBI 977 CGTCTC CGTCTC(1/5)
BspHI 42 TCATGA T^CATGA
Cac8I 55 GCATGC GC[ACGT]^[ACGT]GC
Cfr13I 99 GGTCC G^G[ACGT]CC
DpnII 156 GATC ^GATC
DraI 519 TTTAAA TTT^AAA
EarI 46 GAAGAG CTCTTC(1/4)
EcoT22I 774 ATGCAT ATGCA^T
FbaI 155 TGATCA T^GATCA
Fnu4HI 541 GCAGC GC^[ACGT]GC
HindIII 1043 AAGCTT A^AGCTT
ItaI 541 GCAGC GC^[ACGT]GC
Ksp632I 46 GAAGAG CTCTTC(1/4)
MaeI 61 CTAG C^TAG
MboI 156 GATC ^GATC
MslI 439 CACAAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 149 CATATG CA^TATG
NdeII 156 GATC ^GATC
NsiI 774 ATGCAT ATGCA^T
RcaI 42 TCATGA T^CATGA
Sau3AI 156 GATC ^GATC
Sau96I 99 GGTCC G^G[ACGT]CC
SexAI 116 ACCTGGT A^CC[AT]GGT
SfaNI 906 GCATC GCATC(5/9)
SmlI 1123 CTCAAG C^T[CT][GA]AG
SphI 55 GCATGC GCATG^C
TseI 541 GCAGC G^C[AT]GC
Tsp45I 10 GTGAC ^GT[GC]AC
XmnI 1041 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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BmyI 181 GAGCCC G[AGT]GC[ACT]^C
828 GAGCAC
BpmI 657 CTGGAG CTGGAG(16/14)
1007 CTGGAG
BsmFI 77 GGGAC GGGAC(10/14)
891 GTCCC
BsmI 358 GAATGC GAATGC(1/-1)
1108 GAATGC
Bsp1286I 181 GAGCCC G[AGT]GC[ACT]^C
828 GAGCAC
BssKI 117 CCTGG ^CC[ACGT]GG
185 CCTGG
BstNI 117 CCTGG CC^[AT]GG
185 CCTGG
DsaI 717 CCATGG C^C[GA][CT]GG
801 CCATGG
EcoRII 117 CCTGG ^CC[AT]GG
185 CCTGG
EcoT14I 717 CCATGG C^C[AT][AT]GG
801 CCATGG
HaeIII 792 GGCC GG^CC
960 GGCC
MseI 454 TTAA T^TAA
520 TTAA
MvaI 117 CCTGG CC^[AT]GG
185 CCTGG
NcoI 717 CCATGG C^CATGG
801 CCATGG
NspI 55 GCATGC [GA]CATG^[CT]
604 ACATGC
ScrFI 117 CCTGG CC^[ACGT]GG
185 CCTGG
StuI 791 AGGCCT AGG^CCT
959 AGGCCT
StyI 717 CCATGG C^C[AT][AT]GG
801 CCATGG
Tru9I 454 TTAA T^TAA
520 TTAA
TspRI 609 CAGTG CA[GC]TG[ACGT][ACGT]^
655 CACTG
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AcsI AluI ApoI BsaJI BsmAI
BsrI BstF5I DdeI EcoRI FokI
HinfI HphI MaeIII MboII MnlI
NlaIII SfcI TfiI Tsp509I