Restriction map for
pF1KB7728
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BanI BbeI
BbrPI BcgI BciVI BclI BfaI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BseAI BseRI
BsiWI BspEI BspHI BspLU11I BsrBI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI Bsu36I CelII CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
RcaI RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AcsI 523 GAATTC [GA]^AATT[CT]
AflIII 581 ACACGT A^C[GA][CT]GT
ApaI 1305 GGGCCC GGGCC^C
ApoI 523 GAATTC [GA]^AATT[CT]
BanII 1305 GGGCCC G[GA]GC[CT]^C
BbvCI 1377 CCTCAGC CCTCAGC(-5/-2)
BglI 1307 GCCCCACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 380 ACCGGA [AT]^CCGG[AT]
BsiEI 669 CGATCG CG[GA][CT]^CG
BsmBI 190 GAGACG CGTCTC(1/5)
Bsp1407I 1262 TGTACA T^GTACA
BspDI 394 ATCGAT AT^CGAT
BsrDI 1400 GCAATG GCAATG(2/0)
BsrGI 1262 TGTACA T^GTACA
BsrI 237 ACTGG ACTGG(1/-1)
Bst1107I 330 GTATAC GTA^TAC
BstZ17I 330 GTATAC GTA^TAC
CfoI 806 GCGC GCG^C
ClaI 394 ATCGAT AT^CGAT
EaeI 370 CGGCCA [CT]^GGCC[GA]
EcoRI 523 GAATTC G^AATTC
HaeII 805 AGCGCC [GA]GCGC^[CT]
HgaI 192 GACGC GACGC(5/10)
HhaI 806 GCGC GCG^C
HinP1I 806 GCGC G^CGC
MaeIII 905 GTGAC ^GT[ACGT]AC
NcoI 775 CCATGG C^CATGG
PpuMI 470 GGGACCC [GA]G^G[AT]CC[CT]
PvuI 669 CGATCG CGAT^CG
SexAI 794 ACCAGGT A^CC[AT]GGT
SspBI 1262 TGTACA T^GTACA
Tsp45I 905 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 330 GTATAC GT^[AC][GT]AC
1294 GTCTAC
BamHI 383 GGATCC G^GATCC
1064 GGATCC
BbsI 681 GAAGAC GAAGAC(2/6)
307 GTCTTC
BcnI 690 CCGGG CC^[GC]GG
879 CCCGG
BglII 267 AGATCT A^GATCT
1043 AGATCT
BpuAI 681 GAAGAC GAAGAC(2/6)
307 GTCTTC
BsgI 622 CTGCAC GTGCAG(16/14)
1345 GTGCAG
BsmI 450 GAATGC GAATGC(1/-1)
493 GCATTC
BstXI 1041 CCAGATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1208 CCATCTTCCTGG
DraIII 762 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
877 CACCCGGTG
MseI 587 TTAA T^TAA
1388 TTAA
MslI 297 CATCCGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1317 CATCCATGTG
NaeI 320 GCCGGC GCC^GGC
980 GCCGGC
NciI 690 CCGGG CC^[GC]GG
879 CCCGG
NgoMIV 320 GCCGGC G^CCGGC
980 GCCGGC
PstI 325 CTGCAG CTGCA^G
1156 CTGCAG
SfcI 325 CTGCAG C^T[GA][CT]AG
1156 CTGCAG
Tru9I 587 TTAA T^TAA
1388 TTAA
Tsp509I 524 AATT ^AATT
1386 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BbvI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BssKI BstF5I BstNI BstYI
Cac8I Cfr10I Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeII MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PflMI PleI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TfiI
TseI TspRI TthHB8I Van91I XhoII