Restriction map for
pF1KA1909
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# NO CUT ENZYMES ( 79 enzymes) #
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Acc65I AccIII AclI AflII AflIII
AgeI AscI AseI AsnI Asp718I
AviII BbeI BbrPI BclI BfrI
BsaBI BseAI BsiWI BsmI BspDI
BspEI BspHI BspLU11I BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
Cfr10I ClaI CpoI DraI DraIII
EcoT22I FbaI FseI FspI HpaI
KasI KpnI MamI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfoI SfuI SgrAI SnaBI
Sse8387I SspI SwaI XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 3127 GTCTAC GT^[AC][GT]AC
AocI 2049 CCTCAGG CC^T[ACGT]AGG
AspI 3463 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 339 CCTAGG C^CTAGG
BamHI 3302 GGATCC G^GATCC
BcgI 483 CGATGTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2473 GGATAC GTATCC(6/5)
BglII 238 AGATCT A^GATCT
BlnI 339 CCTAGG C^CTAGG
BsaAI 2680 TACGTG [CT]AC^GT[GA]
BsaWI 3404 ACCGGA [AT]^CCGG[AT]
Bsp1407I 3134 TGTACA T^GTACA
BsrBI 1557 CCGCTC CCGCTC(-3/-3)
BsrGI 3134 TGTACA T^GTACA
BstEII 2268 GGTAACC G^GT[ACGT]ACC
BstPI 2268 GGTAACC G^GT[ACGT]ACC
BstXI 20 CCATGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2049 CCTCAGG CC^T[ACGT]AGG
EagI 3155 CGGCCG C^GGCCG
EclXI 3155 CGGCCG C^GGCCG
Eco52I 3155 CGGCCG C^GGCCG
Eco81I 2049 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2268 GGTAACC G^GT[ACGT]ACC
EcoRI 1092 GAATTC G^AATTC
EcoRV 3700 GATATC GAT^ATC
HincII 964 GTTGAC GT[CT]^[GA]AC
HindII 964 GTTGAC GT[CT]^[GA]AC
HindIII 1180 AAGCTT A^AGCTT
KspI 2970 CCGCGG CCGC^GG
MseI 1153 TTAA T^TAA
PflFI 3463 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 2970 CCGCGG CCGC^GG
SfiI 2073 GGCCACCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 2242 ACTAGT A^CTAGT
SphI 2313 GCATGC GCATG^C
SspBI 3134 TGTACA T^GTACA
Tru9I 1153 TTAA T^TAA
Tth111I 3463 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 171 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 18 enzymes) #
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AatII 574 GACGTC GACGT^C
3124 GACGTC
AhdI 162 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2035 GACCCACTGTC
Alw44I 952 GTGCAC G^TGCAC
1330 GTGCAC
Aor51HI 1977 AGCGCT AGC^GCT
2612 AGCGCT
ApaLI 952 GTGCAC G^TGCAC
1330 GTGCAC
Asp700I 406 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1092 GAATTCATTC
AspEI 162 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2035 GACCCACTGTC
BsrDI 457 CATTGC GCAATG(2/0)
2942 GCAATG
DrdI 1554 GACCCGCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3475 GACAGCATCGTC
Eam1105I 162 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2035 GACCCACTGTC
Eco47III 1977 AGCGCT AGC^GCT
2612 AGCGCT
NspI 2313 GCATGC [GA]CATG^[CT]
3060 GCATGT
PshAI 574 GACGTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3285 GACCGATGTC
SacI 936 GAGCTC GAGCT^C
2896 GAGCTC
SapI 1255 GAAGAGC GCTCTTC(1/4)
2662 GAAGAGC
SmlI 2851 CTCAAG C^T[CT][GA]AG
3195 CTTGAG
TfiI 886 GAATC G^A[AT]TC
3341 GAATC
XmnI 406 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1092 GAATTCATTC
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# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
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AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StuI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI