Restriction map for
pF1KB8336
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AviII AvrII BbeI BbrPI
BbvCI BciVI BclI BfrI BglI
BlnI BsaAI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeII MfeI MroI MseI MunI
NaeI NarI NcoI NgoMIV NheI
NotI NsiI NspV PacI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Van91I
XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AcsI 739 AAATTT [GA]^AATT[CT]
AfaI 1175 GTAC GT^AC
Alw44I 1117 GTGCAC G^TGCAC
AocI 538 CCTGAGG CC^T[ACGT]AGG
ApaLI 1117 GTGCAC G^TGCAC
ApoI 739 AAATTT [GA]^AATT[CT]
AspI 582 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 670 GGTGCC G^G[CT][GA]CC
BcnI 1208 CCGGG CC^[GC]GG
BfaI 689 CTAG C^TAG
BglII 575 AGATCT A^GATCT
BsaBI 1452 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 484 GAGACC GGTCTC(1/5)
BsmAI 484 GAGAC GTCTC(1/5)
BsrDI 123 CATTGC GCAATG(2/0)
BssSI 190 CACGAG CACGAG(-5/-1)
BstXI 28 CCAGTGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 538 CCTGAGG CC^T[ACGT]AGG
DsaI 34 CCGTGG C^C[GA][CT]GG
Eco81I 538 CCTGAGG CC^T[ACGT]AGG
EcoNI 90 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 843 GGCGCT [GA]GCGC^[CT]
MaeI 689 CTAG C^TAG
MamI 1452 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI 1208 CCGGG CC^[GC]GG
NdeI 884 CATATG CA^TATG
NruI 810 TCGCGA TCG^CGA
NspI 529 GCATGT [GA]CATG^[CT]
PflFI 582 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 796 AGGTCCT [GA]G^G[AT]CC[CT]
RsaI 1175 GTAC GT^AC
SacI 1341 GAGCTC GAGCT^C
SexAI 941 ACCAGGT A^CC[AT]GGT
Tsp509I 740 AATT ^AATT
Tth111I 582 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 1148 ACGCGT A^C[GA][CT]GT
1232 ACGCGT
BalI 2 TGGCCA TGG^CCA
697 TGGCCA
BcgI 211 CGAGCGTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
923 GCACTCACCTCG
BlpI 1259 GCTCAGC GC^T[ACGT]AGC
1427 GCTCAGC
Bpu1102I 1259 GCTCAGC GC^T[ACGT]AGC
1427 GCTCAGC
BsgI 140 CTGCAC GTGCAG(16/14)
1395 GTGCAG
BsrBI 345 GAGCGG CCGCTC(-3/-3)
1129 GAGCGG
CelII 1259 GCTCAGC GC^T[ACGT]AGC
1427 GCTCAGC
EcoT14I 251 CCAAGG C^C[AT][AT]GG
507 CCTTGG
FauI 514 CCCGC CCCGC(4/6)
1113 GCGGG
HgaI 1147 GACGC GACGC(5/10)
1231 GACGC
MluI 1148 ACGCGT A^CGCGT
1232 ACGCGT
MluNI 2 TGGCCA TGG^CCA
697 TGGCCA
MscI 2 TGGCCA TGG^CCA
697 TGGCCA
MslI 1304 CATATCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1458 CATCACGGTG
MspA1I 359 CCGCTG C[AC]G^C[GT]G
1111 CAGCGG
PaeR7I 675 CTCGAG C^TCGAG
847 CTCGAG
PstI 232 CTGCAG CTGCA^G
891 CTGCAG
SfcI 232 CTGCAG C^T[GA][CT]AG
891 CTGCAG
SmlI 675 CTCGAG C^T[CT][GA]AG
847 CTCGAG
StyI 251 CCAAGG C^C[AT][AT]GG
507 CCTTGG
TaqI 676 TCGA T^CGA
848 TCGA
TthHB8I 676 TCGA T^CGA
848 TCGA
XhoI 675 CTCGAG C^TCGAG
847 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
Asp700I AspHI AvaI AvaII BamHI
BanII BbsI BbvI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI StuI TfiI
TseI Tsp45I TspRI XhoII XmnI