Restriction map for
pF1KB7791
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AhdI AocI
ApoI AscI AseI AsnI Asp718I
AspEI AviII AvrII BalI BbrPI
BbsI BcgI BciVI BclI BfaI
BfrI BglII BlnI BpuAI BsaAI
BsaBI BsaI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI HincII HindII
HindIII HpaI KpnI KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfuI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
TthHB8I XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcyI 914 GGCGCC G[GA]^CG[CT]C
AfaI 236 GTAC GT^AC
AgeI 1270 ACCGGT A^CCGGT
ApaI 870 GGGCCC GGGCC^C
AspI 804 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 442 GGATCC G^GATCC
BbeI 914 GGCGCC GGCGC^C
BbvCI 829 GCTGAGG CCTCAGC(-5/-2)
BlpI 664 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 664 GCTCAGC GC^T[ACGT]AGC
BsaHI 914 GGCGCC G[GA]^CG[CT]C
BsaWI 1270 ACCGGT [AT]^CCGG[AT]
BsgI 1348 CTGCAC GTGCAG(16/14)
BsmBI 562 GAGACG CGTCTC(1/5)
BspHI 177 TCATGA T^CATGA
BsrBI 1276 GAGCGG CCGCTC(-3/-3)
BstAPI 1208 GCAGCGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 27 CCAGGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 664 GCTCAGC GC^T[ACGT]AGC
EcoNI 753 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 914 GGCGCC G[GA]^CG[CT]C
KasI 914 GGCGCC G^GCGCC
MaeII 800 ACGT A^CGT
MaeIII 802 GTGAC ^GT[ACGT]AC
MseI 542 TTAA T^TAA
NaeI 911 GCCGGC GCC^GGC
NarI 914 GGCGCC GG^CGCC
NcoI 594 CCATGG C^CATGG
NgoMIV 911 GCCGGC G^CCGGC
PflFI 804 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1365 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1270 ACCGGT A^CCGGT
PvuII 951 CAGCTG CAG^CTG
RcaI 177 TCATGA T^CATGA
RsaI 236 GTAC GT^AC
SacI 202 GAGCTC GAGCT^C
SapI 535 GCTCTTC GCTCTTC(1/4)
SexAI 1076 ACCTGGT A^CC[AT]GGT
SfaNI 1338 GCATC GCATC(5/9)
SfoI 914 GGCGCC GGC^GCC
Tru9I 542 TTAA T^TAA
Tsp45I 802 GTGAC ^GT[GC]AC
Tsp509I 149 AATT ^AATT
Tth111I 804 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1365 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Alw44I 1096 GTGCAC G^TGCAC
1264 GTGCAC
Aor51HI 137 AGCGCT AGC^GCT
971 AGCGCT
ApaLI 1096 GTGCAC G^TGCAC
1264 GTGCAC
BsiEI 487 CGGCCG CG[GA][CT]^CG
853 CGGCCG
BspMI 574 ACCTGC ACCTGC(4/8)
1244 ACCTGC
EagI 487 CGGCCG C^GGCCG
853 CGGCCG
EarI 536 CTCTTC CTCTTC(1/4)
676 CTCTTC
EclXI 487 CGGCCG C^GGCCG
853 CGGCCG
Eco47III 137 AGCGCT AGC^GCT
971 AGCGCT
Eco52I 487 CGGCCG C^GGCCG
853 CGGCCG
HphI 337 GGTGA GGTGA(8/7)
1273 GGTGA
Ksp632I 536 CTCTTC CTCTTC(1/4)
676 CTCTTC
PpuMI 362 AGGACCT [GA]G^G[AT]CC[CT]
926 GGGACCT
SgrAI 910 CGCCGGCG C[GA]^CCGG[CT]G
1269 CACCGGTG
SmaI 327 CCCGGG CCC^GGG
635 CCCGGG
StuI 182 AGGCCT AGG^CCT
833 AGGCCT
XcmI 99 CCAGCATGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
379 CCAGTGAAAGCCTGG
XmaCI 327 CCCGGG C^CCGGG
635 CCCGGG
XmaI 327 CCCGGG C^CCGGG
635 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
Asp700I AspHI AvaI AvaII BanI
BanII BbvI BcnI BglI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssHII BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PstI Sau3AI Sau96I ScrFI SfcI
StyI TfiI TseI TspRI XhoII
XmnI