##################################
# NO CUT ENZYMES (  198 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AgeI        AhdI        Alw44I      AlwI        
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AvaI        AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BcnI        BfaI        BfrI        BglII       BlnI        
BmyI        BpuAI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstYI       BstZ17I     Bsu36I      
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HinfI       HpaI        
HpaII       HphI        KasI        KpnI        Ksp632I     
KspI        MaeI        MboII       MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MspA1I      MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        TfiI        Tru9I       Tsp45I      TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AflIII        144  ACGTGT               A^C[GA][CT]GT
AlwNI         178  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspI          157  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbvI          247  GCTGC                GCAGC(8/12)
BclI          293  TGATCA               T^GATCA
BglI          211  GCCTTGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI           13  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I       13  GCTCAGC              GC^T[ACGT]AGC
BsaAI         143  TACGTG               [CT]AC^GT[GA]
BsaBI         288  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspMI          21  ACCTGC               ACCTGC(4/8)
BsrI           86  CCAGT                ACTGG(1/-1)
BstF5I        195  GGATG                GGATG(2/0)
BstXI         317  CCATCATCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I         216  GCAGGC               GC[ACGT]^[ACGT]GC
CelII          13  GCTCAGC              GC^T[ACGT]AGC
DdeI           14  CTCAG                C^T[ACGT]AG
DpnII         294  GATC                 ^GATC
EcoNI          22  CCTGCTGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          293  TGATCA               T^GATCA
Fnu4HI        247  GCTGC                GC^[ACGT]GC
FokI          195  GGATG                GGATG(9/13)
HaeIII         67  GGCC                 GG^CC
ItaI          247  GCTGC                GC^[ACGT]GC
MaeIII         89  GTAAC                ^GT[ACGT]AC
MamI          288  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          294  GATC                 ^GATC
NdeII         294  GATC                 ^GATC
PflFI         157  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Sau3AI        294  GATC                 ^GATC
SfcI          100  CTACAG               C^T[GA][CT]AG
TseI          247  GCTGC                G^C[AT]GC
Tsp509I       233  AATT                 ^AATT
TspRI         243  CAGTG                CA[GC]TG[ACGT][ACGT]^
Tth111I       157  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

BpmI            8  CTCCAG               CTGGAG(16/14)
              166  CTGGAG              
BseRI          71  CTCCTC               GAGGAG(10/8)
              124  GAGGAG              
DraII          57  GGGTCCC              [GA]G^G[ACGT]CC[CT]
              161  AGGTCCT             
EcoO109I       57  GGGTCCC              [GA]G^G[ACGT]CC[CT]
              161  AGGTCCT             
MaeII         144  ACGT                 A^CGT
              152  ACGT                
MslI          141  CATACGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              318  CATCATCGTG          
NlaIII         81  CATG                 CATG^
              297  CATG                
PpuMI          57  GGGTCCC              [GA]G^G[AT]CC[CT]
              161  AGGTCCT             
SfaNI         208  GATGC                GCATC(5/9)
              226  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   16 enzymes) #
#########################################

AluI        AvaII       BsaJI       BsiYI       BslI        
BsmFI       BssKI       BstNI       Cfr13I      EcoRII      
MnlI        MvaI        MwoI        NlaIV       Sau96I      
ScrFI