Restriction map for
pF1KE0546
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BamHI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BseRI BsgI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI KspI MaeI
MamI MfeI MseI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp509I Tth111I
XbaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccII 99 CGCG CG^CG
AccIII 392 TCCGGA T^CCGGA
AflIII 98 ACGCGT A^C[GA][CT]GT
AhdI 196 GACACGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 714 GGATC GGATC(4/5)
AlwNI 499 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 196 GACACGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 357 TGGCCA TGG^CCA
BbeI 163 GGCGCC GGCGC^C
BbsI 454 GTCTTC GAAGAC(2/6)
BglI 257 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 505 CTGGAG CTGGAG(16/14)
BpuAI 454 GTCTTC GAAGAC(2/6)
BsaWI 392 TCCGGA [AT]^CCGG[AT]
BseAI 392 TCCGGA T^CCGGA
BsiEI 449 CGGCCG CG[GA][CT]^CG
BspEI 392 TCCGGA T^CCGGA
BspMI 498 GCAGGT ACCTGC(4/8)
BsrBI 474 CCGCTC CCGCTC(-3/-3)
BsrDI 732 CATTGC GCAATG(2/0)
BsrFI 447 ACCGGC [GA]^CCGG[CT]
BstUI 99 CGCG CG^CG
BstYI 714 GGATCT [GA]^GATC[CT]
Cfr10I 447 ACCGGC [GA]^CCGG[CT]
DpnII 715 GATC ^GATC
DsaI 615 CCGTGG C^C[GA][CT]GG
EagI 449 CGGCCG C^GGCCG
Eam1105I 196 GACACGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 449 CGGCCG C^GGCCG
Eco52I 449 CGGCCG C^GGCCG
EcoNI 648 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 773 CCTTGG C^C[AT][AT]GG
HincII 625 GTCAAC GT[CT]^[GA]AC
HindII 625 GTCAAC GT[CT]^[GA]AC
KasI 163 GGCGCC G^GCGCC
MboI 715 GATC ^GATC
MflI 714 GGATCT [GA]^GATC[CT]
MluI 98 ACGCGT A^CGCGT
MluNI 357 TGGCCA TGG^CCA
MroI 392 TCCGGA T^CCGGA
MscI 357 TGGCCA TGG^CCA
MspA1I 532 CAGCTG C[AC]G^C[GT]G
MvnI 99 CGCG CG^CG
NarI 163 GGCGCC GG^CGCC
NdeII 715 GATC ^GATC
NspI 629 ACATGC [GA]CATG^[CT]
PaeR7I 145 CTCGAG C^TCGAG
PvuII 532 CAGCTG CAG^CTG
SacI 682 GAGCTC GAGCT^C
SapI 679 GAAGAGC GCTCTTC(1/4)
Sau3AI 715 GATC ^GATC
SfoI 163 GGCGCC GGC^GCC
SmaI 329 CCCGGG CCC^GGG
StyI 773 CCTTGG C^C[AT][AT]GG
XcmI 35 CCACCAAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 145 CTCGAG C^TCGAG
XhoII 714 GGATCT [GA]^GATC[CT]
XmaCI 329 CCCGGG C^CCGGG
XmaI 329 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 163 GGCGCC G[GA]^CG[CT]C
201 GGCGTC
AvaII 471 GGACC G^G[AT]CC
827 GGTCC
BsaHI 163 GGCGCC G[GA]^CG[CT]C
201 GGCGTC
BsaI 30 GGTCTC GGTCTC(1/5)
548 GGTCTC
BsmAI 31 GTCTC GTCTC(1/5)
549 GTCTC
BsmFI 194 GGGAC GGGAC(10/14)
867 GTCCC
EarI 184 GAAGAG CTCTTC(1/4)
679 GAAGAG
FauI 131 CCCGC CCCGC(4/6)
87 GCGGG
Hin1I 163 GGCGCC G[GA]^CG[CT]C
201 GGCGTC
Ksp632I 184 GAAGAG CTCTTC(1/4)
679 GAAGAG
MaeIII 421 GTGAC ^GT[ACGT]AC
754 GTCAC
MslI 279 CACGCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
610 CATATCCGTG
NlaIII 630 CATG CATG^
639 CATG
PflMI 35 CCACCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
531 CCAGCTGGTGG
TaqI 146 TCGA T^CGA
665 TCGA
TfiI 517 GAATC G^A[AT]TC
541 GATTC
Tsp45I 421 GTGAC ^GT[GC]AC
754 GTCAC
TspRI 374 CAGTG CA[GC]TG[ACGT][ACGT]^
467 CACTG
TthHB8I 146 TCGA T^CGA
665 TCGA
Van91I 35 CCACCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
531 CCAGCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI ApaI AspHI
AvaI BanI BanII BbrPI BbvI
BcnI BmyI BsaAI BsaJI BsiHKAI
BsiYI BslI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI Cac8I
CfoI Cfr13I DdeI DraII DraIII
EaeI EcoO109I EcoRII Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MboII MnlI MspI MvaI
MwoI NciI NlaIV PleI PmaCI
PmlI RsaI Sau96I ScrFI SfaNI
SmlI TseI