Restriction map for
pF1KB7348
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflIII AgeI AhdI AlwNI Aor51HI
AscI Asp700I Asp718I AspEI AvaI
AviII AvrII BalI BbrPI BcgI
BcnI BglI BlnI BsaAI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
BspEI BspLU11I BsrBI BssHII BssSI
Bst1107I BstBI BstUI BstXI BstZ17I
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
FseI FspI HindIII KpnI KspI
MluI MluNI MroI MscI MvnI
NaeI NciI NgoMIV NotI NruI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SwaI Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AcyI 26 GGCGCC G[GA]^CG[CT]C
AocI 12 CCTGAGG CC^T[ACGT]AGG
ApaI 1272 GGGCCC GGGCC^C
AseI 1003 ATTAAT AT^TAAT
AsnI 1003 ATTAAT AT^TAAT
AspI 1717 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 2898 GGTCC G^G[AT]CC
BamHI 2685 GGATCC G^GATCC
BbeI 26 GGCGCC GGCGC^C
BbvCI 2893 GCTGAGG CCTCAGC(-5/-2)
BciVI 3015 GTATCC GTATCC(6/5)
BglII 3031 AGATCT A^GATCT
BlpI 565 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 565 GCTAAGC GC^T[ACGT]AGC
BsaBI 3213 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 26 GGCGCC G[GA]^CG[CT]C
BsmFI 2842 GTCCC GGGAC(10/14)
Bsp1407I 1146 TGTACA T^GTACA
BspDI 1012 ATCGAT AT^CGAT
BspMI 2934 GCAGGT ACCTGC(4/8)
BsrFI 3146 ACCGGC [GA]^CCGG[CT]
BsrGI 1146 TGTACA T^GTACA
BstAPI 543 GCAGATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1432 GGTGACC G^GT[ACGT]ACC
BstPI 1432 GGTGACC G^GT[ACGT]ACC
Bsu36I 12 CCTGAGG CC^T[ACGT]AGG
CelII 565 GCTAAGC GC^T[ACGT]AGC
Cfr10I 3146 ACCGGC [GA]^CCGG[CT]
ClaI 1012 ATCGAT AT^CGAT
DsaI 1565 CCATGG C^C[GA][CT]GG
Eco81I 12 CCTGAGG CC^T[ACGT]AGG
EcoNI 1382 CCTGTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1432 GGTGACC G^GT[ACGT]ACC
EcoT14I 1565 CCATGG C^C[AT][AT]GG
HaeII 26 GGCGCC [GA]GCGC^[CT]
Hin1I 26 GGCGCC G[GA]^CG[CT]C
HincII 904 GTTAAC GT[CT]^[GA]AC
HindII 904 GTTAAC GT[CT]^[GA]AC
HpaI 904 GTTAAC GTT^AAC
KasI 26 GGCGCC G^GCGCC
MaeII 1125 ACGT A^CGT
MamI 3213 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 500 CAGCTG C[AC]G^C[GT]G
NarI 26 GGCGCC GG^CGCC
NcoI 1565 CCATGG C^CATGG
NheI 969 GCTAGC G^CTAGC
PflFI 1717 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1003 ATTAAT AT^TAAT
PvuII 500 CAGCTG CAG^CTG
SapI 664 GAAGAGC GCTCTTC(1/4)
SfoI 26 GGCGCC GGC^GCC
SpeI 2263 ACTAGT A^CTAGT
SspBI 1146 TGTACA T^GTACA
StuI 1739 AGGCCT AGG^CCT
StyI 1565 CCATGG C^C[AT][AT]GG
Tth111I 1717 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 629 CCAAAGAATGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflII 106 CTTAAG C^TTAAG
1825 CTTAAG
BanI 26 GGCGCC G^G[CT][GA]CC
1093 GGTGCC
BfrI 106 CTTAAG C^TTAAG
1825 CTTAAG
BpmI 1847 CTCCAG CTGGAG(16/14)
32 CTGGAG
BsaI 928 GAGACC GGTCTC(1/5)
2185 GGTCTC
BsgI 1649 CTGCAC GTGCAG(16/14)
415 GTGCAG
DraII 1271 GGGGCCC [GA]G^G[ACGT]CC[CT]
1272 GGGCCCT
EarI 664 GAAGAG CTCTTC(1/4)
2919 GAAGAG
EcoO109I 1271 GGGGCCC [GA]G^G[ACGT]CC[CT]
1272 GGGCCCT
EcoRI 2002 GAATTC G^AATTC
2295 GAATTC
EcoRV 873 GATATC GAT^ATC
2743 GATATC
FauI 1571 GCGGG CCCGC(4/6)
2365 GCGGG
HapII 76 CCGG C^CGG
3147 CCGG
HgaI 1025 GACGC GACGC(5/10)
1561 GCGTC
HpaII 76 CCGG C^CGG
3147 CCGG
Ksp632I 664 GAAGAG CTCTTC(1/4)
2919 GAAGAG
MfeI 2507 CAATTG C^AATTG
2846 CAATTG
MspI 76 CCGG C^CGG
3147 CCGG
MunI 2507 CAATTG C^AATTG
2846 CAATTG
NdeI 131 CATATG CA^TATG
2155 CATATG
PstI 209 CTGCAG CTGCA^G
2488 CTGCAG
SacI 2150 GAGCTC GAGCT^C
3187 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
Alw44I AlwI ApaLI ApoI AspHI
BanII BbsI BbvI BclI BfaI
BmyI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmI Bsp1286I
BspHI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII EcoT22I FbaI
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NsiI NspI PleI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII