Restriction map for
pF1KB7893
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI AlwI AlwNI
AocI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AvrII BalI
BamHI BbrPI BbsI BcgI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsgI BsiEI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV FseI HgaI HindIII
HpaI KpnI KspI MamI MfeI
MflI MluI MluNI MscI MseI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tru9I
Tth111I Van91I XbaI XhoII
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 210 CGCG CG^CG
AccIII 1442 TCCGGA T^CCGGA
AcyI 613 GGCGCC G[GA]^CG[CT]C
Alw44I 1388 GTGCAC G^TGCAC
ApaI 69 GGGCCC GGGCC^C
ApaLI 1388 GTGCAC G^TGCAC
AviII 380 TGCGCA TGC^GCA
BbeI 613 GGCGCC GGCGC^C
BbvCI 151 GCTGAGG CCTCAGC(-5/-2)
BclI 1034 TGATCA T^GATCA
BfaI 1225 CTAG C^TAG
BsaHI 613 GGCGCC G[GA]^CG[CT]C
BseAI 1442 TCCGGA T^CCGGA
BsiWI 314 CGTACG C^GTACG
BsmI 1565 GCATTC GAATGC(1/-1)
BspEI 1442 TCCGGA T^CCGGA
BsrDI 215 GCAATG GCAATG(2/0)
BsrFI 1712 ACCGGC [GA]^CCGG[CT]
BstAPI 1692 GCAGACTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1336 GGTGACC G^GT[ACGT]ACC
BstPI 1336 GGTGACC G^GT[ACGT]ACC
BstUI 210 CGCG CG^CG
Cfr10I 1712 ACCGGC [GA]^CCGG[CT]
DsaI 413 CCACGG C^C[GA][CT]GG
EcoNI 1194 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1336 GGTGACC G^GT[ACGT]ACC
EcoT22I 1257 ATGCAT ATGCA^T
FbaI 1034 TGATCA T^GATCA
FspI 380 TGCGCA TGC^GCA
Hin1I 613 GGCGCC G[GA]^CG[CT]C
HincII 1726 GTCAAC GT[CT]^[GA]AC
HindII 1726 GTCAAC GT[CT]^[GA]AC
KasI 613 GGCGCC G^GCGCC
MaeI 1225 CTAG C^TAG
MroI 1442 TCCGGA T^CCGGA
MslI 1342 CATCGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 210 CGCG CG^CG
NarI 613 GGCGCC GG^CGCC
NsiI 1257 ATGCAT ATGCA^T
PaeR7I 640 CTCGAG C^TCGAG
SacI 697 GAGCTC GAGCT^C
SapI 699 GCTCTTC GCTCTTC(1/4)
ScaI 1553 AGTACT AGT^ACT
SexAI 947 ACCTGGT A^CC[AT]GGT
SfoI 613 GGCGCC GGC^GCC
SphI 1259 GCATGC GCATG^C
StuI 572 AGGCCT AGG^CCT
TfiI 759 GAATC G^A[AT]TC
XcmI 74 CCAGTGGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 640 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 1135 GAATTT [GA]^AATT[CT]
1500 GAATTT
ApoI 1135 GAATTT [GA]^AATT[CT]
1500 GAATTT
Asp700I 1365 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1497 GAAGAATTTC
BglI 62 GCCCGTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
71 GCCCCAGTGGC
BsaWI 1392 ACCGGA [AT]^CCGG[AT]
1442 TCCGGA
BsmBI 1463 CGTCTC CGTCTC(1/5)
802 GAGACG
BsrI 74 CCAGT ACTGG(1/-1)
240 CCAGT
DpnII 843 GATC ^GATC
1035 GATC
EaeI 1470 TGGCCG [CT]^GGCC[GA]
1714 CGGCCA
EarI 700 CTCTTC CTCTTC(1/4)
1404 GAAGAG
Ksp632I 700 CTCTTC CTCTTC(1/4)
1404 GAAGAG
MaeII 1268 ACGT A^CGT
1724 ACGT
MboI 843 GATC ^GATC
1035 GATC
NdeII 843 GATC ^GATC
1035 GATC
NspI 740 GCATGT [GA]CATG^[CT]
1259 GCATGC
PpuMI 944 GGGACCT [GA]G^G[AT]CC[CT]
956 GGGACCT
Sau3AI 843 GATC ^GATC
1035 GATC
XmnI 1365 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1497 GAAGAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DraII DrdI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboII MnlI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV PleI PstI PvuII
RsaI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I XmaCI
XmaI