Restriction map for
pF1KE0545
##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI AlwI
AlwNI AocI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BbeI BcgI
BciVI BfrI BglII BlnI BsaBI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstYI BstZ17I Bsu36I ClaI
CpoI DraI Eam1105I EarI Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FseI FspI HindIII HinfI
KasI KpnI Ksp632I KspI MamI
MfeI MflI MluI MroI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PleI PmeI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
TfiI Tsp509I XbaI XcmI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 499 GTCTAC GT^[AC][GT]AC
Alw44I 565 GTGCAC G^TGCAC
Aor51HI 888 AGCGCT AGC^GCT
ApaLI 565 GTGCAC G^TGCAC
BanII 117 GAGCCC G[GA]GC[CT]^C
BbrPI 175 CACGTG CAC^GTG
BbsI 802 GAAGAC GAAGAC(2/6)
BbvCI 609 CCTCAGC CCTCAGC(-5/-2)
BclI 575 TGATCA T^GATCA
BfaI 85 CTAG C^TAG
BglI 406 GCCATGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 961 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 961 GCTCAGC GC^T[ACGT]AGC
BpuAI 802 GAAGAC GAAGAC(2/6)
BsaAI 175 CACGTG [CT]AC^GT[GA]
BsaI 295 GAGACC GGTCTC(1/5)
BsgI 727 CTGCAC GTGCAG(16/14)
BsiEI 552 CGGCCG CG[GA][CT]^CG
BspMI 78 GCAGGT ACCTGC(4/8)
BsrDI 594 CATTGC GCAATG(2/0)
BssHII 252 GCGCGC G^CGCGC
BstAPI 717 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 961 GCTCAGC GC^T[ACGT]AGC
DraII 473 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 633 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1012 GACCCCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 854 CCACGG C^C[GA][CT]GG
EagI 552 CGGCCG C^GGCCG
EclXI 552 CGGCCG C^GGCCG
Eco47III 888 AGCGCT AGC^GCT
Eco52I 552 CGGCCG C^GGCCG
EcoO109I 473 AGGACCC [GA]G^G[ACGT]CC[CT]
FbaI 575 TGATCA T^GATCA
HincII 877 GTTAAC GT[CT]^[GA]AC
HindII 877 GTTAAC GT[CT]^[GA]AC
HpaI 877 GTTAAC GTT^AAC
MaeI 85 CTAG C^TAG
MseI 878 TTAA T^TAA
PflMI 669 CCAGTGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 175 CACGTG CAC^GTG
PmlI 175 CACGTG CAC^GTG
PpuMI 473 AGGACCC [GA]G^G[AT]CC[CT]
SexAI 860 ACCAGGT A^CC[AT]GGT
SmaI 122 CCCGGG CCC^GGG
Tru9I 878 TTAA T^TAA
Van91I 669 CCAGTGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 122 CCCGGG C^CCGGG
XmaI 122 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 265 GACGCC G[GA]^CG[CT]C
400 GACGCC
AfaI 663 GTAC GT^AC
708 GTAC
AspHI 460 GTGCTC G[AT]GC[AT]^C
565 GTGCAC
AspI 784 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
859 GACCAGGTC
AvaI 122 CCCGGG C^[CT]CG[GA]G
274 CCCGAG
BalI 320 TGGCCA TGG^CCA
906 TGGCCA
BanI 166 GGCACC G^G[CT][GA]CC
238 GGCACC
BpmI 424 CTGGAG CTGGAG(16/14)
777 CTGGAG
BsaHI 265 GACGCC G[GA]^CG[CT]C
400 GACGCC
BsiHKAI 460 GTGCTC G[AT]GC[AT]^C
565 GTGCAC
BsmAI 295 GAGAC GTCTC(1/5)
829 GTCTC
BsoBI 122 CCCGGG C^[CT]CG[GA]G
274 CCCGAG
BsrBI 205 CCGCTC CCGCTC(-3/-3)
847 GAGCGG
BsrFI 34 GCCGGC [GA]^CCGG[CT]
450 GCCGGC
BsrI 904 ACTGG ACTGG(1/-1)
669 CCAGT
BstXI 170 CCAACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
909 CCACATCCCTGG
Cfr10I 34 GCCGGC [GA]^CCGG[CT]
450 GCCGGC
HaeII 737 GGCGCT [GA]GCGC^[CT]
888 AGCGCT
Hin1I 265 GACGCC G[GA]^CG[CT]C
400 GACGCC
MaeII 176 ACGT A^CGT
244 ACGT
MboII 802 GAAGA GAAGA(8/7)
212 TCTTC
MluNI 320 TGGCCA TGG^CCA
906 TGGCCA
MscI 320 TGGCCA TGG^CCA
906 TGGCCA
NaeI 34 GCCGGC GCC^GGC
450 GCCGGC
NgoMIV 34 GCCGGC G^CCGGC
450 GCCGGC
PflFI 784 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
859 GACCAGGTC
PvuII 67 CAGCTG CAG^CTG
718 CAGCTG
RsaI 663 GTAC GT^AC
708 GTAC
SmlI 487 CTCAAG C^T[CT][GA]AG
745 CTCAAG
StuI 332 AGGCCT AGG^CCT
413 AGGCCT
TspRI 234 CAGTG CA[GC]TG[ACGT][ACGT]^
670 CAGTG
Tth111I 784 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
859 GACCAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AluI AvaII BbvI
BcnI BmyI BsaJI BseRI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HpaII HphI ItaI
MaeIII MboI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI TaqI TseI Tsp45I
TthHB8I