Restriction map for
pF1KE0196
##################################
# NO CUT ENZYMES ( 158 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfrI BglI BlnI BlpI
BmyI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmBI BsmFI Bsp1286I
BspDI BspEI BspLU11I BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV FauI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MaeII MamI MluI
MroI MspI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
StuI SwaI Tth111I Van91I XbaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AciI 73 GCGG CCGC(-3/-1)
AlwNI 443 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 1202 CTCGAG C^[CT]CG[GA]G
AvaII 1102 GGACC G^G[AT]CC
BclI 641 TGATCA T^GATCA
BfaI 1108 CTAG C^TAG
BglII 28 AGATCT A^GATCT
BsmI 438 GAATGC GAATGC(1/-1)
BsoBI 1202 CTCGAG C^[CT]CG[GA]G
Bsp1407I 2 TGTACA T^GTACA
BspMI 650 GCAGGT ACCTGC(4/8)
BsrGI 2 TGTACA T^GTACA
BstAPI 442 GCAGTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 28 AGATCT [GA]^GATC[CT]
Cfr13I 1102 GGACC G^G[ACGT]CC
DsaI 589 CCATGG C^C[GA][CT]GG
EcoT14I 589 CCATGG C^C[AT][AT]GG
FbaI 641 TGATCA T^GATCA
HincII 712 GTCAAC GT[CT]^[GA]AC
HindII 712 GTCAAC GT[CT]^[GA]AC
MaeI 1108 CTAG C^TAG
MfeI 1221 CAATTG C^AATTG
MflI 28 AGATCT [GA]^GATC[CT]
MspA1I 340 CAGCTG C[AC]G^C[GT]G
MunI 1221 CAATTG C^AATTG
NcoI 589 CCATGG C^CATGG
NdeI 1013 CATATG CA^TATG
NlaIV 631 GGAGCC GG[ACGT]^[ACGT]CC
PaeR7I 1202 CTCGAG C^TCGAG
PstI 665 CTGCAG CTGCA^G
PvuII 340 CAGCTG CAG^CTG
Sau96I 1102 GGACC G^G[ACGT]CC
SfcI 665 CTGCAG C^T[GA][CT]AG
SmlI 1202 CTCGAG C^T[CT][GA]AG
SphI 602 GCATGC GCATG^C
SspBI 2 TGTACA T^GTACA
StyI 589 CCATGG C^C[AT][AT]GG
TaqI 1203 TCGA T^CGA
TthHB8I 1203 TCGA T^CGA
XcmI 455 CCATTAAACTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1202 CTCGAG C^TCGAG
XhoII 28 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 3 GTAC GT^AC
852 GTAC
BalI 34 TGGCCA TGG^CCA
586 TGGCCA
BsgI 331 GTGCAG GTGCAG(16/14)
820 GTGCAG
BsmAI 232 GAGAC GTCTC(1/5)
516 GAGAC
BspHI 774 TCATGA T^CATGA
801 TCATGA
BsrDI 606 GCAATG GCAATG(2/0)
1004 GCAATG
EaeI 34 TGGCCA [CT]^GGCC[GA]
586 TGGCCA
HphI 145 GGTGA GGTGA(8/7)
1162 GGTGA
MluNI 34 TGGCCA TGG^CCA
586 TGGCCA
MscI 34 TGGCCA TGG^CCA
586 TGGCCA
MseI 244 TTAA T^TAA
458 TTAA
MslI 584 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
622 CACTACTGTG
NspI 602 GCATGC [GA]CATG^[CT]
1081 GCATGT
PleI 1244 GACTC GAGTC(4/5)
164 GAGTC
RcaI 774 TCATGA T^CATGA
801 TCATGA
RsaI 3 GTAC GT^AC
852 GTAC
Tru9I 244 TTAA T^TAA
458 TTAA
TspRI 673 CACTG CA[GC]TG[ACGT][ACGT]^
956 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AcsI AluI ApoI BbsI BbvI
BpmI BpuAI BsaJI BsiYI BslI
BsrI BssKI BstF5I BstNI Cac8I
DdeI DpnII EcoRII EcoT22I Fnu4HI
FokI HaeIII HinfI ItaI MaeIII
MboI MboII MnlI MvaI MwoI
NdeII NlaIII NsiI Sau3AI ScrFI
SfaNI TfiI TseI Tsp45I Tsp509I