Restriction map for
pF1KE0182
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AviII AvrII BamHI
BbeI BbrPI BciVI BclI BcnI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiHKAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI Eam1105I EarI Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI Hin1I HincII
HindII HpaI KasI KpnI Ksp632I
KspI MaeII MamI MfeI MflI
MluI MroI MseI MspA1I MunI
NarI NciI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI ScaI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI SspBI SspI
SwaI Tru9I Van91I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 101 CGCG CG^CG
AfaI 438 GTAC GT^AC
Asp700I 592 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 261 CTCGGG C^[CT]CG[GA]G
BanI 151 GGTGCC G^G[CT][GA]CC
BbvCI 577 GCTGAGG CCTCAGC(-5/-2)
BcgI 476 CGACTGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 613 CTAG C^TAG
BpmI 232 CTGGAG CTGGAG(16/14)
BseRI 580 GAGGAG GAGGAG(10/8)
BsiEI 97 CGGCCG CG[GA][CT]^CG
BsmFI 273 GTCCC GGGAC(10/14)
BsmI 522 GAATGC GAATGC(1/-1)
BsoBI 261 CTCGGG C^[CT]CG[GA]G
BspMI 107 ACCTGC ACCTGC(4/8)
BsrDI 282 CATTGC GCAATG(2/0)
BsrFI 95 GCCGGC [GA]^CCGG[CT]
BstF5I 417 GGATG GGATG(2/0)
BstUI 101 CGCG CG^CG
Cfr10I 95 GCCGGC [GA]^CCGG[CT]
DraII 642 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 502 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 155 CCATGG C^C[GA][CT]GG
EagI 97 CGGCCG C^GGCCG
EclXI 97 CGGCCG C^GGCCG
Eco52I 97 CGGCCG C^GGCCG
EcoO109I 642 AGGCCCT [GA]G^G[ACGT]CC[CT]
FokI 417 GGATG GGATG(9/13)
HapII 96 CCGG C^CGG
HgaI 466 GACGC GACGC(5/10)
HindIII 319 AAGCTT A^AGCTT
HinfI 124 GATTC G^A[ACGT]TC
HpaII 96 CCGG C^CGG
MaeI 613 CTAG C^TAG
MspI 96 CCGG C^CGG
MvnI 101 CGCG CG^CG
NaeI 95 GCCGGC GCC^GGC
NcoI 155 CCATGG C^CATGG
NgoMIV 95 GCCGGC G^CCGGC
RsaI 438 GTAC GT^AC
SbfI 657 CCTGCAGG CCTGCA^GG
SexAI 503 ACCTGGT A^CC[AT]GGT
SfaNI 256 GATGC GCATC(5/9)
SfiI 634 GGCCTCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 657 CCTGCAGG CCTGCA^GG
TfiI 124 GATTC G^A[AT]TC
Tsp509I 675 AATT ^AATT
XmnI 592 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AciI 100 CCGC CCGC(-3/-1)
399 GCGG
AluI 320 AGCT AG^CT
615 AGCT
AlwI 248 GGATC GGATC(4/5)
401 GGATC
AlwNI 562 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
641 CAGGCCCTG
Aor51HI 86 AGCGCT AGC^GCT
116 AGCGCT
AspI 162 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
193 GACGATGTC
AvaII 141 GGACC G^G[AT]CC
450 GGACC
BalI 35 TGGCCA TGG^CCA
392 TGGCCA
BanII 172 GAGCCC G[GA]GC[CT]^C
339 GGGCTC
BbsI 241 GAAGAC GAAGAC(2/6)
357 GAAGAC
BglI 635 GCCTCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
644 GCCCTGAAGGC
BmyI 172 GAGCCC G[AGT]GC[ACT]^C
339 GGGCTC
BpuAI 241 GAAGAC GAAGAC(2/6)
357 GAAGAC
Bsp1286I 172 GAGCCC G[AGT]GC[ACT]^C
339 GGGCTC
CfoI 87 GCGC GCG^C
117 GCGC
DpnII 249 GATC ^GATC
402 GATC
Eco47III 86 AGCGCT AGC^GCT
116 AGCGCT
EcoT14I 65 CCTTGG C^C[AT][AT]GG
155 CCATGG
HaeII 86 AGCGCT [GA]GCGC^[CT]
116 AGCGCT
HhaI 87 GCGC GCG^C
117 GCGC
HinP1I 87 GCGC G^CGC
117 GCGC
MboI 249 GATC ^GATC
402 GATC
MluNI 35 TGGCCA TGG^CCA
392 TGGCCA
MscI 35 TGGCCA TGG^CCA
392 TGGCCA
NdeII 249 GATC ^GATC
402 GATC
PflFI 162 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
193 GACGATGTC
PstI 112 CTGCAG CTGCA^G
658 CTGCAG
Sau3AI 249 GATC ^GATC
402 GATC
SfcI 112 CTGCAG C^T[GA][CT]AG
658 CTGCAG
StuI 543 AGGCCT AGG^CCT
633 AGGCCT
StyI 65 CCTTGG C^C[AT][AT]GG
155 CCATGG
TspRI 277 CACTG CA[GC]TG[ACGT][ACGT]^
369 CAGTG
Tth111I 162 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
193 GACGATGTC
XcmI 23 CCATAGGTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
38 CCACCATCTTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
BbvI BsaI BsaJI BsiYI BslI
BsmAI BsrI BssKI BstNI BstXI
Cac8I Cfr13I DdeI EaeI EcoRII
Fnu4HI HaeIII HphI ItaI MaeIII
MboII MnlI MslI MvaI MwoI
NlaIII NlaIV Sau96I ScrFI TaqI
TseI Tsp45I TthHB8I