Restriction map for
pF1KE0105
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# NO CUT ENZYMES ( 178 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BpmI BsaAI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
HphI KasI KpnI Ksp632I KspI
MaeI MaeII MfeI MluI MluNI
MroI MscI MseI MslI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 34 CGCG CG^CG
AcsI 431 AAATTT [GA]^AATT[CT]
AfaI 147 GTAC GT^AC
AlwI 316 GGATC GGATC(4/5)
ApoI 431 AAATTT [GA]^AATT[CT]
BanI 496 GGTGCC G^G[CT][GA]CC
BanII 117 GAGCCC G[GA]GC[CT]^C
BbsI 532 GAAGAC GAAGAC(2/6)
BcnI 120 CCCGG CC^[GC]GG
BmyI 117 GAGCCC G[AGT]GC[ACT]^C
BpuAI 532 GAAGAC GAAGAC(2/6)
BsaBI 300 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 25 GAGGAG GAGGAG(10/8)
BsmI 283 GCATTC GAATGC(1/-1)
Bsp1286I 117 GAGCCC G[AGT]GC[ACT]^C
BsrDI 279 CATTGC GCAATG(2/0)
BstF5I 501 CATCC GGATG(2/0)
BstUI 34 CGCG CG^CG
BstYI 316 GGATCT [GA]^GATC[CT]
DpnII 317 GATC ^GATC
FokI 501 CATCC GGATG(9/13)
HapII 121 CCGG C^CGG
HpaII 121 CCGG C^CGG
MaeIII 130 GTCAC ^GT[ACGT]AC
MamI 300 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 317 GATC ^GATC
MflI 316 GGATCT [GA]^GATC[CT]
MspI 121 CCGG C^CGG
MvnI 34 CGCG CG^CG
NciI 120 CCCGG CC^[GC]GG
NdeII 317 GATC ^GATC
NlaIV 496 GGTGCC GG[ACGT]^[ACGT]CC
PvuII 134 CAGCTG CAG^CTG
RsaI 147 GTAC GT^AC
Sau3AI 317 GATC ^GATC
ScaI 146 AGTACT AGT^ACT
SfaNI 77 GCATC GCATC(5/9)
SfcI 333 CTATAG C^T[GA][CT]AG
TaqI 321 TCGA T^CGA
Tsp45I 130 GTCAC ^GT[GC]AC
Tsp509I 432 AATT ^AATT
TspRI 202 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 321 TCGA T^CGA
XhoII 316 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AvaII 99 GGACC G^G[AT]CC
390 GGTCC
BlpI 114 GCTGAGC GC^T[ACGT]AGC
216 GCTCAGC
Bpu1102I 114 GCTGAGC GC^T[ACGT]AGC
216 GCTCAGC
BsrI 449 ACTGG ACTGG(1/-1)
144 CCAGT
Cac8I 10 GCGGGC GC[ACGT]^[ACGT]GC
184 GCCAGC
CelII 114 GCTGAGC GC^T[ACGT]AGC
216 GCTCAGC
Cfr13I 99 GGACC G^G[ACGT]CC
390 GGTCC
FauI 181 CCCGC CCCGC(4/6)
10 GCGGG
HaeII 87 GGCGCT [GA]GCGC^[CT]
262 GGCGCT
HinfI 166 GAGTC G^A[ACGT]TC
208 GAGTC
MboII 532 GAAGA GAAGA(8/7)
80 TCTTC
MnlI 25 GAGG CCTC(7/6)
28 GAGG
MspA1I 15 CAGCGG C[AC]G^C[GT]G
134 CAGCTG
PleI 166 GAGTC GAGTC(4/5)
208 GAGTC
Sau96I 99 GGACC G^G[ACGT]CC
390 GGTCC
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BssKI BstNI CfoI DdeI
EcoRII Fnu4HI HaeIII HhaI HinP1I
ItaI MvaI MwoI NlaIII ScrFI
TseI