Restriction map for
pF1KE0103
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI AlwNI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BclI
BfrI BglII BlnI BsaBI BsiWI
BsmI Bsp1407I BspDI BspHI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I Bsu36I ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI HpaI
KpnI KspI MamI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SbfI ScaI SfcI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccI 598 GTCGAC GT^[AC][GT]AC
AccIII 243 TCCGGA T^CCGGA
AcyI 277 GGCGCC G[GA]^CG[CT]C
AflIII 39 ACATGT A^C[GA][CT]GT
Alw44I 265 GTGCAC G^TGCAC
ApaLI 265 GTGCAC G^TGCAC
AspHI 265 GTGCAC G[AT]GC[AT]^C
AvaI 96 CTCGGG C^[CT]CG[GA]G
BamHI 983 GGATCC G^GATCC
BbeI 277 GGCGCC GGCGC^C
BbvCI 193 GCTGAGG CCTCAGC(-5/-2)
BcgI 473 GCAAAAACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 814 CTAG C^TAG
BglI 1138 GCCGGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 84 GCTGAGC GC^T[ACGT]AGC
BpmI 631 CTGGAG CTGGAG(16/14)
Bpu1102I 84 GCTGAGC GC^T[ACGT]AGC
BsaHI 277 GGCGCC G[GA]^CG[CT]C
BsaWI 243 TCCGGA [AT]^CCGG[AT]
BseAI 243 TCCGGA T^CCGGA
BsgI 856 CTGCAC GTGCAG(16/14)
BsiEI 483 CGACCG CG[GA][CT]^CG
BsiHKAI 265 GTGCAC G[AT]GC[AT]^C
BsmBI 424 GAGACG CGTCTC(1/5)
BsoBI 96 CTCGGG C^[CT]CG[GA]G
BspEI 243 TCCGGA T^CCGGA
BspLU11I 39 ACATGT A^CATGT
BsrBI 744 GAGCGG CCGCTC(-3/-3)
BsrDI 1134 CATTGC GCAATG(2/0)
BsrFI 1138 GCCGGC [GA]^CCGG[CT]
BstEII 805 GGTGACC G^GT[ACGT]ACC
BstPI 805 GGTGACC G^GT[ACGT]ACC
BstYI 983 GGATCC [GA]^GATC[CT]
CelII 84 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1138 GCCGGC [GA]^CCGG[CT]
DraIII 232 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1105 CTCTTC CTCTTC(1/4)
EcoO65I 805 GGTGACC G^GT[ACGT]ACC
HaeII 277 GGCGCC [GA]GCGC^[CT]
Hin1I 277 GGCGCC G[GA]^CG[CT]C
HindIII 295 AAGCTT A^AGCTT
KasI 277 GGCGCC G^GCGCC
Ksp632I 1105 CTCTTC CTCTTC(1/4)
MaeI 814 CTAG C^TAG
MflI 983 GGATCC [GA]^GATC[CT]
MroI 243 TCCGGA T^CCGGA
MseI 50 TTAA T^TAA
NaeI 1138 GCCGGC GCC^GGC
NarI 277 GGCGCC GG^CGCC
NcoI 515 CCATGG C^CATGG
NgoMIV 1138 GCCGGC G^CCGGC
NspI 39 ACATGT [GA]CATG^[CT]
PpuMI 635 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 938 CAGCTG CAG^CTG
SalI 598 GTCGAC G^TCGAC
SapI 1104 GCTCTTC GCTCTTC(1/4)
SexAI 1025 ACCTGGT A^CC[AT]GGT
SfaNI 227 GCATC GCATC(5/9)
SfoI 277 GGCGCC GGC^GCC
StuI 582 AGGCCT AGG^CCT
Tru9I 50 TTAA T^TAA
XhoII 983 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 44 enzymes) #
###################################
AccII 6 CGCG CG^CG
166 CGCG
ApaI 765 GGGCCC GGGCC^C
1124 GGGCCC
AspI 592 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1024 GACCTGGTC
AvaII 321 GGTCC G^G[AT]CC
636 GGACC
BanI 277 GGCGCC G^G[CT][GA]CC
1179 GGTGCC
BbrPI 352 CACGTG CAC^GTG
775 CACGTG
BbsI 174 GAAGAC GAAGAC(2/6)
918 GAAGAC
BbvI 798 GCAGC GCAGC(8/12)
937 GCAGC
BciVI 840 GGATAC GTATCC(6/5)
696 GTATCC
BcnI 29 CCCGG CC^[GC]GG
794 CCCGG
BpuAI 174 GAAGAC GAAGAC(2/6)
918 GAAGAC
BsaAI 352 CACGTG [CT]AC^GT[GA]
775 CACGTG
BsaI 91 GAGACC GGTCTC(1/5)
1022 GAGACC
BspMI 411 GCAGGT ACCTGC(4/8)
563 GCAGGT
BstUI 6 CGCG CG^CG
166 CGCG
CfoI 167 GCGC GCG^C
278 GCGC
DpnII 307 GATC ^GATC
984 GATC
DrdI 712 GACTTGCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
808 GACCAGCTAGTC
EcoT14I 515 CCATGG C^C[AT][AT]GG
995 CCTTGG
FauI 801 GCGGG CCCGC(4/6)
933 GCGGG
HhaI 167 GCGC GCG^C
278 GCGC
HinP1I 167 GCGC G^CGC
278 GCGC
HincII 598 GTCGAC GT[CT]^[GA]AC
1030 GTCAAC
HindII 598 GTCGAC GT[CT]^[GA]AC
1030 GTCAAC
HinfI 385 GAGTC G^A[ACGT]TC
526 GAGTC
MaeII 353 ACGT A^CGT
776 ACGT
MaeIII 781 GTCAC ^GT[ACGT]AC
806 GTGAC
MboI 307 GATC ^GATC
984 GATC
MspA1I 799 CAGCGG C[AC]G^C[GT]G
938 CAGCTG
MvnI 6 CGCG CG^CG
166 CGCG
MwoI 1074 GCTGACCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1138 GCCGGCCTGGC
NciI 29 CCCGG CC^[GC]GG
794 CCCGG
NdeII 307 GATC ^GATC
984 GATC
PflFI 592 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1024 GACCTGGTC
PflMI 231 CCACAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
310 CCAAACTATGG
PleI 385 GAGTC GAGTC(4/5)
526 GAGTC
PmaCI 352 CACGTG CAC^GTG
775 CACGTG
PmlI 352 CACGTG CAC^GTG
775 CACGTG
Sau3AI 307 GATC ^GATC
984 GATC
StyI 515 CCATGG C^C[AT][AT]GG
995 CCTTGG
TseI 798 GCAGC G^C[AT]GC
937 GCAGC
Tsp45I 781 GTCAC ^GT[GC]AC
806 GTGAC
Tth111I 592 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1024 GACCTGGTC
Van91I 231 CCACAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
310 CCAAACTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI AlwI BalI
BanII BmyI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI Cac8I
Cfr13I DdeI DraII DsaI EaeI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HpaII HphI ItaI MboII
MluNI MnlI MscI MslI MspI
MvaI NlaIII NlaIV RsaI Sau96I
ScrFI TaqI Tsp509I TspRI TthHB8I