Restriction map for
pF1KE0344
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# NO CUT ENZYMES ( 101 enzymes) #
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AatII Acc65I AclI AcsI AflII
AflIII AgeI AhdI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BcgI BclI BfrI BglII BlnI
BsiWI BsmI BspDI BspHI BspLU11I
BsrBI BsrDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I ClaI CpoI DraI DrdI
Eam1105I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI MfeI MflI MluI MseI
MunI NcoI NdeI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SbfI SexAI
SfuI SgrAI SmlI SnaBI SphI
Sse8387I SspI SwaI TfiI Tru9I
Tsp45I Tth111I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 967 GTCTAC GT^[AC][GT]AC
AccII 20 CGCG CG^CG
AccIII 2044 TCCGGA T^CCGGA
AlwI 1608 GATCC GGATC(4/5)
Aor51HI 1679 AGCGCT AGC^GCT
BbvCI 426 GCTGAGG CCTCAGC(-5/-2)
BciVI 1638 GTATCC GTATCC(6/5)
BsaAI 1267 TACGTG [CT]AC^GT[GA]
BsaBI 792 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2044 TCCGGA T^CCGGA
BsmBI 1786 GAGACG CGTCTC(1/5)
Bsp1407I 1451 TGTACA T^GTACA
BspEI 2044 TCCGGA T^CCGGA
BsrGI 1451 TGTACA T^GTACA
BstUI 20 CGCG CG^CG
DraIII 1101 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1679 AGCGCT AGC^GCT
EcoT14I 923 CCAAGG C^C[AT][AT]GG
HincII 1015 GTTGAC GT[CT]^[GA]AC
HindII 1015 GTTGAC GT[CT]^[GA]AC
KspI 19 CCGCGG CCGC^GG
MaeIII 1415 GTTAC ^GT[ACGT]AC
MamI 792 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2044 TCCGGA T^CCGGA
MslI 1929 CATGGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 20 CGCG CG^CG
NaeI 1472 GCCGGC GCC^GGC
NgoMIV 1472 GCCGGC G^CCGGC
NheI 1965 GCTAGC G^CTAGC
SacII 19 CCGCGG CCGC^GG
SapI 27 GCTCTTC GCTCTTC(1/4)
ScaI 1688 AGTACT AGT^ACT
SfaNI 113 GCATC GCATC(5/9)
SmaI 1276 CCCGGG CCC^GGG
SpeI 258 ACTAGT A^CTAGT
SspBI 1451 TGTACA T^GTACA
StuI 93 AGGCCT AGG^CCT
StyI 923 CCAAGG C^C[AT][AT]GG
Tsp509I 702 AATT ^AATT
XmaCI 1276 CCCGGG C^CCGGG
XmaI 1276 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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Alw44I 254 GTGCAC G^TGCAC
994 GTGCAC
ApaLI 254 GTGCAC G^TGCAC
994 GTGCAC
AvaI 106 CCCGAG C^[CT]CG[GA]G
1276 CCCGGG
BbeI 1289 GGCGCC GGCGC^C
1730 GGCGCC
BsaI 175 GAGACC GGTCTC(1/5)
613 GAGACC
BsaWI 1982 TCCGGT [AT]^CCGG[AT]
2044 TCCGGA
BseRI 1924 CTCCTC GAGGAG(10/8)
1744 GAGGAG
BsgI 670 CTGCAC GTGCAG(16/14)
1954 GTGCAG
BsoBI 106 CCCGAG C^[CT]CG[GA]G
1276 CCCGGG
BstAPI 771 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1956 GCAGGCCCTGC
EagI 47 CGGCCG C^GGCCG
1474 CGGCCG
EarI 28 CTCTTC CTCTTC(1/4)
1360 CTCTTC
EclXI 47 CGGCCG C^GGCCG
1474 CGGCCG
Eco52I 47 CGGCCG C^GGCCG
1474 CGGCCG
EcoNI 1584 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1854 CCTGGCCCAGG
KasI 1289 GGCGCC G^GCGCC
1730 GGCGCC
Ksp632I 28 CTCTTC CTCTTC(1/4)
1360 CTCTTC
MaeII 1268 ACGT A^CGT
1667 ACGT
NarI 1289 GGCGCC GG^CGCC
1730 GGCGCC
PstI 781 CTGCAG CTGCA^G
1588 CTGCAG
SfiI 94 GGCCTGTATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
927 GGCCCTGCTGGCC
SfoI 1289 GGCGCC GGC^GCC
1730 GGCGCC
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# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
AocI ApaI AspHI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI Eco81I EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MboI MboII MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfcI TaqI TseI
TspRI TthHB8I Van91I XcmI