Restriction map for
pF1KB7767
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AccI AccIII AclI AflII AflIII
AgeI AhdI Aor51HI AscI AseI
AsnI AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI DsaI Eam1105I
EarI Eco47III EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
Ksp632I KspI MamI MfeI MluI
MroI MseI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NruI NsiI NspI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Tth111I
XbaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 808 GACGTC GACGT^C
Acc65I 76 GGTACC G^GTACC
AccII 426 CGCG CG^CG
AcyI 808 GACGTC G[GA]^CG[CT]C
Alw44I 754 GTGCAC G^TGCAC
AlwI 499 GGATC GGATC(4/5)
AocI 463 CCTCAGG CC^T[ACGT]AGG
ApaLI 754 GTGCAC G^TGCAC
Asp700I 531 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 76 GGTACC G^GTACC
AspHI 754 GTGCAC G[AT]GC[AT]^C
BalI 170 TGGCCA TGG^CCA
BfaI 302 CTAG C^TAG
BglI 649 GCCTGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 818 AGATCT A^GATCT
BsaHI 808 GACGTC G[GA]^CG[CT]C
BsaI 1287 GGTCTC GGTCTC(1/5)
BsgI 1266 CTGCAC GTGCAG(16/14)
BsiEI 189 CGGCCG CG[GA][CT]^CG
BsiHKAI 754 GTGCAC G[AT]GC[AT]^C
BsmBI 1075 GAGACG CGTCTC(1/5)
BspMI 1056 GCAGGT ACCTGC(4/8)
BsrBI 793 CCGCTC CCGCTC(-3/-3)
BstAPI 1161 GCACAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 426 CGCG CG^CG
Bsu36I 463 CCTCAGG CC^T[ACGT]AGG
EagI 189 CGGCCG C^GGCCG
EclXI 189 CGGCCG C^GGCCG
Eco52I 189 CGGCCG C^GGCCG
Eco81I 463 CCTCAGG CC^T[ACGT]AGG
FauI 251 CCCGC CCCGC(4/6)
HaeII 786 AGCGCC [GA]GCGC^[CT]
HgaI 1202 GCGTC GACGC(5/10)
Hin1I 808 GACGTC G[GA]^CG[CT]C
KpnI 76 GGTACC GGTAC^C
MaeI 302 CTAG C^TAG
MaeII 809 ACGT A^CGT
MluNI 170 TGGCCA TGG^CCA
MscI 170 TGGCCA TGG^CCA
MvnI 426 CGCG CG^CG
NotI 188 GCGGCCGC GC^GGCCGC
PaeR7I 796 CTCGAG C^TCGAG
PleI 567 GACTC GAGTC(4/5)
PpuMI 450 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 436 CTGCAG CTGCA^G
SfcI 436 CTGCAG C^T[GA][CT]AG
SmaI 11 CCCGGG CCC^GGG
SmlI 796 CTCGAG C^T[CT][GA]AG
StuI 467 AGGCCT AGG^CCT
TfiI 730 GATTC G^A[AT]TC
XhoI 796 CTCGAG C^TCGAG
XmaCI 11 CCCGGG C^CCGGG
XmaI 11 CCCGGG C^CCGGG
XmnI 531 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 829 AAATTC [GA]^AATT[CT]
1152 GAATTT
AlwNI 39 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1252 CAGGAACTG
ApoI 829 AAATTC [GA]^AATT[CT]
1152 GAATTT
BbsI 276 GAAGAC GAAGAC(2/6)
1279 GTCTTC
BpuAI 276 GAAGAC GAAGAC(2/6)
1279 GTCTTC
BseRI 70 GAGGAG GAGGAG(10/8)
937 GAGGAG
BsmFI 1337 GGGAC GGGAC(10/14)
452 GTCCC
BsrFI 79 ACCGGC [GA]^CCGG[CT]
949 GCCGGT
BstYI 499 GGATCT [GA]^GATC[CT]
818 AGATCT
Cfr10I 79 ACCGGC [GA]^CCGG[CT]
949 GCCGGT
EaeI 170 TGGCCA [CT]^GGCC[GA]
189 CGGCCG
EcoT14I 371 CCAAGG C^C[AT][AT]GG
1034 CCAAGG
HinfI 567 GACTC G^A[ACGT]TC
730 GATTC
MflI 499 GGATCT [GA]^GATC[CT]
818 AGATCT
PflMI 312 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
387 CCAGACAATGG
SexAI 362 ACCAGGT A^CC[AT]GGT
1145 ACCTGGT
StyI 371 CCAAGG C^C[AT][AT]GG
1034 CCAAGG
Van91I 312 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
387 CCAGACAATGG
XcmI 483 CCAGTTTTGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1220 CCATCCTCCAGGTGG
XhoII 499 GGATCT [GA]^GATC[CT]
818 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI ApaI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BsiYI BslI
BsmAI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I