Restriction map for
pF1KE0544
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII BamHI BbeI
BbrPI BcgI BciVI BfrI BglI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII Eam1105I
Eco81I EcoRI EcoRV EcoT22I FseI
FspI HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tsp509I Tth111I TthHB8I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 727 GTCTAC GT^[AC][GT]AC
AfaI 1026 GTAC GT^AC
AlwI 653 GGATC GGATC(4/5)
Aor51HI 620 AGCGCT AGC^GCT
ApaI 44 GGGCCC GGGCC^C
AspHI 142 GAGCAC G[AT]GC[AT]^C
AvaI 706 CCCGAG C^[CT]CG[GA]G
AvrII 252 CCTAGG C^CTAGG
BanII 44 GGGCCC G[GA]GC[CT]^C
BbsI 205 GAAGAC GAAGAC(2/6)
BbvCI 1263 GCTGAGG CCTCAGC(-5/-2)
BclI 432 TGATCA T^GATCA
BcnI 1052 CCGGG CC^[GC]GG
BglII 686 AGATCT A^GATCT
BlnI 252 CCTAGG C^CTAGG
BpuAI 205 GAAGAC GAAGAC(2/6)
BseRI 682 GAGGAG GAGGAG(10/8)
BsgI 217 CTGCAC GTGCAG(16/14)
BsiEI 1163 CGGCCG CG[GA][CT]^CG
BsiHKAI 142 GAGCAC G[AT]GC[AT]^C
BsoBI 706 CCCGAG C^[CT]CG[GA]G
BsrFI 128 ACCGGC [GA]^CCGG[CT]
BssSI 935 CACGAG CACGAG(-5/-1)
BstEII 235 GGTTACC G^GT[ACGT]ACC
BstPI 235 GGTTACC G^GT[ACGT]ACC
BstYI 686 AGATCT [GA]^GATC[CT]
CfoI 621 GCGC GCG^C
Cfr10I 128 ACCGGC [GA]^CCGG[CT]
DrdI 373 GACAGGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1163 CGGCCG C^GGCCG
EclXI 1163 CGGCCG C^GGCCG
Eco47III 620 AGCGCT AGC^GCT
Eco52I 1163 CGGCCG C^GGCCG
EcoNI 1074 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 235 GGTTACC G^GT[ACGT]ACC
FauI 507 GCGGG CCCGC(4/6)
FbaI 432 TGATCA T^GATCA
HaeII 620 AGCGCT [GA]GCGC^[CT]
HhaI 621 GCGC GCG^C
HinP1I 621 GCGC G^CGC
HindIII 229 AAGCTT A^AGCTT
MflI 686 AGATCT [GA]^GATC[CT]
MseI 1141 TTAA T^TAA
NciI 1052 CCGGG CC^[GC]GG
NdeI 1106 CATATG CA^TATG
NspI 796 GCATGT [GA]CATG^[CT]
PflMI 570 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 938 GAGTC GAGTC(4/5)
PpuMI 35 GGGTCCT [GA]G^G[AT]CC[CT]
RsaI 1026 GTAC GT^AC
SfaNI 158 GCATC GCATC(5/9)
StuI 1118 AGGCCT AGG^CCT
TfiI 1100 GATTC G^A[AT]TC
Tru9I 1141 TTAA T^TAA
Van91I 570 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 686 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwNI 871 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
953 CAGCTGCTG
BalI 662 TGGCCA TGG^CCA
824 TGGCCA
BanI 5 GGCACC G^G[CT][GA]CC
930 GGCACC
BfaI 253 CTAG C^TAG
455 CTAG
BmyI 44 GGGCCC G[AGT]GC[ACT]^C
142 GAGCAC
BsmFI 196 GGGAC GGGAC(10/14)
371 GGGAC
Bsp1286I 44 GGGCCC G[AGT]GC[ACT]^C
142 GAGCAC
BspHI 758 TCATGA T^CATGA
860 TCATGA
EarI 22 CTCTTC CTCTTC(1/4)
414 GAAGAG
HinfI 938 GAGTC G^A[ACGT]TC
1100 GATTC
Ksp632I 22 CTCTTC CTCTTC(1/4)
414 GAAGAG
MaeI 253 CTAG C^TAG
455 CTAG
MluNI 662 TGGCCA TGG^CCA
824 TGGCCA
MscI 662 TGGCCA TGG^CCA
824 TGGCCA
MslI 51 CACTGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
159 CATCTATGTG
RcaI 758 TCATGA T^CATGA
860 TCATGA
SmlI 226 CTCAAG C^T[CT][GA]AG
802 CTCAAG
Tsp45I 281 GTGAC ^GT[GC]AC
994 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AvaII BbvI BpmI
BsaI BsaJI BsiYI BslI BsmAI
BsmI BspMI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HpaII HphI ItaI MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
PstI PvuII Sau3AI Sau96I ScrFI
SfcI StyI TseI TspRI XcmI