Restriction map for
pF1KB6433
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AlwI
AlwNI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaII
AviII AvrII BamHI BbrPI BciVI
BclI BfrI BglII BlnI BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BsrI
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstXI BstYI BstZ17I
ClaI CpoI DpnII DraI DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV EcoT22I
FbaI FokI FseI FspI HindIII
HpaI KpnI Ksp632I KspI MamI
MboI MfeI MflI MluI MroI
MunI NdeI NdeII NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
Sau3AI SbfI ScaI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AcsI 313 GAATTC [GA]^AATT[CT]
Alw44I 911 GTGCAC G^TGCAC
ApaLI 911 GTGCAC G^TGCAC
ApoI 313 GAATTC [GA]^AATT[CT]
BbeI 712 GGCGCC GGCGC^C
BbvCI 192 CCTCAGC CCTCAGC(-5/-2)
BcgI 197 GCATCGAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 460 CTAG C^TAG
BglI 260 GCCGAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 156 GCTGAGC GC^T[ACGT]AGC
BpmI 151 CTCCAG CTGGAG(16/14)
Bpu1102I 156 GCTGAGC GC^T[ACGT]AGC
BsaI 581 GAGACC GGTCTC(1/5)
BseRI 300 CTCCTC GAGGAG(10/8)
BsgI 229 CTGCAC GTGCAG(16/14)
BspMI 256 ACCTGC ACCTGC(4/8)
BsrDI 96 GCAATG GCAATG(2/0)
CelII 156 GCTGAGC GC^T[ACGT]AGC
DraIII 232 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 313 GAATTC G^AATTC
HaeII 712 GGCGCC [GA]GCGC^[CT]
HgaI 742 GACGC GACGC(5/10)
HincII 836 GTCAAC GT[CT]^[GA]AC
HindII 836 GTCAAC GT[CT]^[GA]AC
KasI 712 GGCGCC G^GCGCC
MaeI 460 CTAG C^TAG
MaeII 23 ACGT A^CGT
MseI 668 TTAA T^TAA
NaeI 844 GCCGGC GCC^GGC
NarI 712 GGCGCC GG^CGCC
NcoI 353 CCATGG C^CATGG
NgoMIV 844 GCCGGC G^CCGGC
PleI 599 GACTC GAGTC(4/5)
PshAI 829 GACTATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 382 CTGCAG CTGCA^G
SacI 148 GAGCTC GAGCT^C
SexAI 632 ACCTGGT A^CC[AT]GGT
SfoI 712 GGCGCC GGC^GCC
SmaI 736 CCCGGG CCC^GGG
Tru9I 668 TTAA T^TAA
Tsp509I 314 AATT ^AATT
XmaCI 736 CCCGGG C^CCGGG
XmaI 736 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 700 CGCG CG^CG
702 CGCG
AcyI 712 GGCGCC G[GA]^CG[CT]C
742 GACGCC
AocI 808 CCTCAGG CC^T[ACGT]AGG
871 CCTCAGG
BbsI 103 GTCTTC GAAGAC(2/6)
280 GTCTTC
BpuAI 103 GTCTTC GAAGAC(2/6)
280 GTCTTC
BsaHI 712 GGCGCC G[GA]^CG[CT]C
742 GACGCC
BsmAI 581 GAGAC GTCTC(1/5)
673 GAGAC
BsrFI 729 ACCGGC [GA]^CCGG[CT]
844 GCCGGC
BssHII 699 GCGCGC G^CGCGC
701 GCGCGC
BstNI 633 CCTGG CC^[AT]GG
792 CCAGG
BstUI 700 CGCG CG^CG
702 CGCG
Bsu36I 808 CCTCAGG CC^T[ACGT]AGG
871 CCTCAGG
Cfr10I 729 ACCGGC [GA]^CCGG[CT]
844 GCCGGC
Eco81I 808 CCTCAGG CC^T[ACGT]AGG
871 CCTCAGG
EcoRII 633 CCTGG ^CC[AT]GG
792 CCAGG
FauI 903 CCCGC CCCGC(4/6)
779 GCGGG
Hin1I 712 GGCGCC G[GA]^CG[CT]C
742 GACGCC
HphI 27 GGTGA GGTGA(8/7)
323 TCACC
MvaI 633 CCTGG CC^[AT]GG
792 CCAGG
MvnI 700 CGCG CG^CG
702 CGCG
NspI 127 ACATGC [GA]CATG^[CT]
565 ACATGC
PvuII 34 CAGCTG CAG^CTG
154 CAGCTG
SfcI 138 CTACAG C^T[GA][CT]AG
382 CTGCAG
TfiI 30 GAATC G^A[AT]TC
796 GATTC
TspRI 141 CAGTG CA[GC]TG[ACGT][ACGT]^
858 CAGTG
XcmI 353 CCATGGGGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
822 CCAGCCTGACTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI ApaI AspHI
AvaI BalI BanI BanII BbvI
BcnI BmyI BsaJI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BssKI
Cac8I CfoI Cfr13I DdeI DraII
DsaI EaeI EcoO109I EcoT14I Fnu4HI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MaeIII MboII MluNI
MnlI MscI MslI MspA1I MspI
MwoI NciI NlaIII NlaIV RsaI
Sau96I ScrFI SfaNI StyI TaqI
TseI Tsp45I TthHB8I