Restriction map for
pF1KE0161
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# NO CUT ENZYMES ( 151 enzymes) #
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Acc65I AccI AccII AccIII AclI
AcsI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspHI
AvaI AvaII AviII AvrII BbeI
BbrPI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraI DsaI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI FauI FbaI FseI FspI
HaeII HhaI HinP1I HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MluI MroI MspA1I MunI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tsp509I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AatII 421 GACGTC GACGT^C
AcyI 421 GACGTC G[GA]^CG[CT]C
AfaI 711 GTAC GT^AC
AhdI 220 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 199 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 336 GGGCCC GGGCC^C
AspEI 220 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 88 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 753 TGGCCA TGG^CCA
BamHI 438 GGATCC G^GATCC
BanI 171 GGTGCC G^G[CT][GA]CC
BanII 336 GGGCCC G[GA]GC[CT]^C
BbvCI 114 CCTCAGC CCTCAGC(-5/-2)
BbvI 659 GCAGC GCAGC(8/12)
BsaHI 421 GACGTC G[GA]^CG[CT]C
BsaI 598 GAGACC GGTCTC(1/5)
BseRI 222 CTCCTC GAGGAG(10/8)
BsmAI 598 GAGAC GTCTC(1/5)
BsmFI 618 GGGAC GGGAC(10/14)
BsmI 609 GCATTC GAATGC(1/-1)
BspMI 178 ACCTGC ACCTGC(4/8)
BsrFI 601 ACCGGC [GA]^CCGG[CT]
BsrI 766 ACTGG ACTGG(1/-1)
BstAPI 31 GCAATCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 482 CATCC GGATG(2/0)
BstNI 156 CCAGG CC^[AT]GG
Cfr10I 601 ACCGGC [GA]^CCGG[CT]
DraII 335 GGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 154 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 349 GACTTCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 220 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 335 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRII 156 CCAGG ^CC[AT]GG
EcoRV 626 GATATC GAT^ATC
EcoT14I 443 CCAAGG C^C[AT][AT]GG
EcoT22I 492 ATGCAT ATGCA^T
FokI 482 CATCC GGATG(9/13)
HgaI 92 GCGTC GACGC(5/10)
Hin1I 421 GACGTC G[GA]^CG[CT]C
HincII 94 GTCAAC GT[CT]^[GA]AC
HindII 94 GTCAAC GT[CT]^[GA]AC
HindIII 522 AAGCTT A^AGCTT
MluNI 753 TGGCCA TGG^CCA
MscI 753 TGGCCA TGG^CCA
MseI 526 TTAA T^TAA
MslI 531 CACTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 156 CCAGG CC^[AT]GG
NdeI 579 CATATG CA^TATG
NsiI 492 ATGCAT ATGCA^T
NspI 49 ACATGC [GA]CATG^[CT]
PflFI 88 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 220 GACTC GAGTC(4/5)
RsaI 711 GTAC GT^AC
SexAI 155 ACCAGGT A^CC[AT]GGT
SfaNI 119 GCATC GCATC(5/9)
SfcI 362 CTGTAG C^T[GA][CT]AG
StuI 69 AGGCCT AGG^CCT
StyI 443 CCAAGG C^C[AT][AT]GG
TaqI 122 TCGA T^CGA
TfiI 548 GATTC G^A[AT]TC
Tru9I 526 TTAA T^TAA
TseI 659 GCAGC G^C[AT]GC
Tth111I 88 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 122 TCGA T^CGA
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BbsI 228 GTCTTC GAAGAC(2/6)
259 GTCTTC
BcnI 321 CCGGG CC^[GC]GG
333 CCGGG
BmyI 172 GTGCCC G[AGT]GC[ACT]^C
336 GGGCCC
BpmI 654 CTCCAG CTGGAG(16/14)
65 CTGGAG
BpuAI 228 GTCTTC GAAGAC(2/6)
259 GTCTTC
BsaJI 333 CCGGGG C^C[ACGT][ACGT]GG
443 CCAAGG
Bsp1286I 172 GTGCCC G[AGT]GC[ACT]^C
336 GGGCCC
BstYI 438 GGATCC [GA]^GATC[CT]
636 AGATCC
EaeI 281 TGGCCG [CT]^GGCC[GA]
753 TGGCCA
HinfI 220 GACTC G^A[ACGT]TC
548 GATTC
MaeII 163 ACGT A^CGT
422 ACGT
MflI 438 GGATCC [GA]^GATC[CT]
636 AGATCC
MwoI 31 GCAATCTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
256 GCTGTCTTCGC
NciI 321 CCGGG CC^[GC]GG
333 CCGGG
NlaIII 50 CATG CATG^
495 CATG
XhoII 438 GGATCC [GA]^GATC[CT]
636 AGATCC
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AluI AlwI BssKI Cac8I
Cfr13I DdeI DpnII Fnu4HI HaeIII
HapII HpaII HphI ItaI MaeIII
MboI MboII MnlI MspI NdeII
NlaIV Sau3AI Sau96I ScrFI Tsp45I
TspRI XcmI