Restriction map for
pF1KB8653
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaWI BseAI BsgI
BsiEI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DdeI DraI
DraII DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KspI MaeI MaeIII MamI
MfeI MluI MroI MseI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tsp45I Tsp509I Tth111I XbaI
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 512 GGTACC G^GTACC
AflIII 266 ACGTGT A^C[GA][CT]GT
AluI 205 AGCT AG^CT
Asp718I 512 GGTACC G^GTACC
AspHI 226 GTGCTC G[AT]GC[AT]^C
AvaI 440 CCCGGG C^[CT]CG[GA]G
BalI 377 TGGCCA TGG^CCA
BamHI 618 GGATCC G^GATCC
BpmI 573 CTCCAG CTGGAG(16/14)
BsaAI 595 TACGTG [CT]AC^GT[GA]
BsaI 393 GGTCTC GGTCTC(1/5)
BseRI 166 GAGGAG GAGGAG(10/8)
BsiHKAI 226 GTGCTC G[AT]GC[AT]^C
BsiWI 14 CGTACG C^GTACG
BsmFI 427 GGGAC GGGAC(10/14)
BsoBI 440 CCCGGG C^[CT]CG[GA]G
BsrBI 20 GAGCGG CCGCTC(-3/-3)
BstF5I 78 CATCC GGATG(2/0)
BstYI 618 GGATCC [GA]^GATC[CT]
DrdI 322 GACATCGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 218 CCGTGG C^C[GA][CT]GG
EarI 538 CTCTTC CTCTTC(1/4)
EcoT14I 404 CCAAGG C^C[AT][AT]GG
FokI 78 CATCC GGATG(9/13)
KpnI 512 GGTACC GGTAC^C
Ksp632I 538 CTCTTC CTCTTC(1/4)
MflI 618 GGATCC [GA]^GATC[CT]
MluNI 377 TGGCCA TGG^CCA
MscI 377 TGGCCA TGG^CCA
MslI 108 CATAGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 508 CAGCGG C[AC]G^C[GT]G
NaeI 651 GCCGGC GCC^GGC
NgoMIV 651 GCCGGC G^CCGGC
NspI 578 GCATGC [GA]CATG^[CT]
PflMI 380 CCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 201 CTGCAG CTGCA^G
SfaNI 176 GCATC GCATC(5/9)
SfcI 201 CTGCAG C^T[GA][CT]AG
SmaI 440 CCCGGG CCC^GGG
SphI 578 GCATGC GCATG^C
StyI 404 CCAAGG C^C[AT][AT]GG
TfiI 365 GATTC G^A[AT]TC
Van91I 380 CCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 618 GGATCC [GA]^GATC[CT]
XmaCI 440 CCCGGG C^CCGGG
XmaI 440 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 85 CGCG CG^CG
588 CGCG
AcyI 37 GGCGCC G[GA]^CG[CT]C
523 GGCGCC
AlwI 619 GATCC GGATC(4/5)
618 GGATC
BanII 601 GAGCCC G[GA]GC[CT]^C
673 GAGCCC
BbeI 37 GGCGCC GGCGC^C
523 GGCGCC
BglI 337 GCCTTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
526 GCCGACTCGGC
BsaHI 37 GGCGCC G[GA]^CG[CT]C
523 GGCGCC
BsmAI 331 GTCTC GTCTC(1/5)
394 GTCTC
BspMI 241 ACCTGC ACCTGC(4/8)
390 GCAGGT
BsrFI 625 GCCGGT [GA]^CCGG[CT]
651 GCCGGC
BstUI 85 CGCG CG^CG
588 CGCG
Cfr10I 625 GCCGGT [GA]^CCGG[CT]
651 GCCGGC
DpnII 282 GATC ^GATC
619 GATC
Hin1I 37 GGCGCC G[GA]^CG[CT]C
523 GGCGCC
HphI 239 TCACC GGTGA(8/7)
476 TCACC
KasI 37 GGCGCC G^GCGCC
523 GGCGCC
MaeII 266 ACGT A^CGT
596 ACGT
MboI 282 GATC ^GATC
619 GATC
MboII 473 TCTTC GAAGA(8/7)
539 TCTTC
MvnI 85 CGCG CG^CG
588 CGCG
NarI 37 GGCGCC GG^CGCC
523 GGCGCC
NdeII 282 GATC ^GATC
619 GATC
NlaIII 555 CATG CATG^
579 CATG
PleI 529 GACTC GAGTC(4/5)
571 GACTC
Sau3AI 282 GATC ^GATC
619 GATC
SfoI 37 GGCGCC GGC^GCC
523 GGCGCC
TaqI 44 TCGA T^CGA
665 TCGA
TthHB8I 44 TCGA T^CGA
665 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AfaI AvaII BanI BbvI
BcnI BmyI BsaJI BsiYI BslI
Bsp1286I BsrI BssKI BstNI Cac8I
CfoI Cfr13I EaeI EcoRII FauI
Fnu4HI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII ItaI
MnlI MspI MvaI MwoI NciI
NlaIV RsaI Sau96I ScrFI TseI
TspRI