Restriction map for
pF1KA0162
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# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AclI AflII AgeI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I AspI AviII AvrII
BbrPI BfrI BlnI BsiEI BsiWI
BspDI BssHII Bst1107I BstZ17I Bsu36I
ClaI CpoI EagI EclXI Eco47III
Eco52I Eco81I EcoRV EcoT22I FseI
FspI MluI NaeI NdeI NgoMIV
NotI NruI NsiI PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SalI
ScaI SgrAI SnaBI SpeI SphI
SspI SwaI Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 1387 GTCTAC GT^[AC][GT]AC
AccIII 3410 TCCGGA T^CCGGA
AflIII 1565 ACATGT A^C[GA][CT]GT
AhdI 2732 GACAGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1054 GGGCCC GGGCC^C
AspEI 2732 GACAGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1540 GAGCTC G[AT]GC[AT]^C
BbeI 1759 GGCGCC GGCGC^C
BcgI 1548 GCAAGCCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 138 TGATCA T^GATCA
BlpI 3387 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3387 GCTGAGC GC^T[ACGT]AGC
BsaAI 4804 CACGTA [CT]AC^GT[GA]
BsaBI 3244 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3410 TCCGGA T^CCGGA
BsiHKAI 1540 GAGCTC G[AT]GC[AT]^C
BsmBI 2739 CGTCTC CGTCTC(1/5)
BspEI 3410 TCCGGA T^CCGGA
BspLU11I 1565 ACATGT A^CATGT
BssSI 1669 CACGAG CACGAG(-5/-1)
BstBI 4213 TTCGAA TT^CGAA
BstEII 3051 GGTCACC G^GT[ACGT]ACC
BstPI 3051 GGTCACC G^GT[ACGT]ACC
CelII 3387 GCTGAGC GC^T[ACGT]AGC
DraI 1917 TTTAAA TTT^AAA
DrdI 3697 GACAATGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2732 GACAGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3051 GGTCACC G^GT[ACGT]ACC
EcoRI 4210 GAATTC G^AATTC
FbaI 138 TGATCA T^GATCA
HaeII 1759 GGCGCC [GA]GCGC^[CT]
HindIII 601 AAGCTT A^AGCTT
HpaI 3456 GTTAAC GTT^AAC
KasI 1759 GGCGCC G^GCGCC
KspI 1688 CCGCGG CCGC^GG
MamI 3244 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2250 CAATTG C^AATTG
MroI 3410 TCCGGA T^CCGGA
MunI 2250 CAATTG C^AATTG
NarI 1759 GGCGCC GG^CGCC
NheI 1450 GCTAGC G^CTAGC
NspV 4213 TTCGAA TT^CGAA
PaeR7I 3031 CTCGAG C^TCGAG
SacI 1540 GAGCTC GAGCT^C
SacII 1688 CCGCGG CCGC^GG
SbfI 3253 CCTGCAGG CCTGCA^GG
SexAI 2099 ACCAGGT A^CC[AT]GGT
SfiI 480 GGCCATGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1759 GGCGCC GGC^GCC
SfuI 4213 TTCGAA TT^CGAA
Sse8387I 3253 CCTGCAGG CCTGCA^GG
XhoI 3031 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Acc65I 66 GGTACC G^GTACC
4484 GGTACC
AccII 621 CGCG CG^CG
1689 CGCG
Asp718I 66 GGTACC G^GTACC
4484 GGTACC
BamHI 1220 GGATCC G^GATCC
4548 GGATCC
BciVI 610 GGATAC GTATCC(6/5)
4423 GGATAC
BsaI 3475 GAGACC GGTCTC(1/5)
4042 GAGACC
BsaWI 3410 TCCGGA [AT]^CCGG[AT]
4481 TCCGGT
Bsp1407I 1568 TGTACA T^GTACA
2601 TGTACA
BspHI 3083 TCATGA T^CATGA
3800 TCATGA
BsrGI 1568 TGTACA T^GTACA
2601 TGTACA
BstUI 621 CGCG CG^CG
1689 CGCG
KpnI 66 GGTACC GGTAC^C
4484 GGTACC
MaeII 1718 ACGT A^CGT
4805 ACGT
MvnI 621 CGCG CG^CG
1689 CGCG
RcaI 3083 TCATGA T^CATGA
3800 TCATGA
SapI 2470 GAAGAGC GCTCTTC(1/4)
3325 GAAGAGC
SmaI 4268 CCCGGG CCC^GGG
4433 CCCGGG
SspBI 1568 TGTACA T^GTACA
2601 TGTACA
StuI 2228 AGGCCT AGG^CCT
2948 AGGCCT
XmaCI 4268 CCCGGG C^CCGGG
4433 CCCGGG
XmaI 4268 CCCGGG C^CCGGG
4433 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BglII
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII