Restriction map for
pF1KE0229
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# NO CUT ENZYMES ( 177 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
AluI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeIII MamI MfeI MluI MslI
MspA1I MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tsp45I TspRI
Tth111I TthHB8I Van91I XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccIII 517 TCCGGA T^CCGGA
AfaI 173 GTAC GT^AC
BalI 410 TGGCCA TGG^CCA
BamHI 587 GGATCC G^GATCC
BanII 239 GAGCCC G[GA]GC[CT]^C
BbvI 568 GCTGC GCAGC(8/12)
BfaI 250 CTAG C^TAG
BmyI 239 GAGCCC G[AGT]GC[ACT]^C
BpmI 809 CTGGAG CTGGAG(16/14)
BsaJI 422 CCAAGG C^C[ACGT][ACGT]GG
BseAI 517 TCCGGA T^CCGGA
BsmAI 812 GAGAC GTCTC(1/5)
BsmFI 76 GTCCC GGGAC(10/14)
Bsp1286I 239 GAGCCC G[AGT]GC[ACT]^C
BspEI 517 TCCGGA T^CCGGA
BsrI 815 ACTGG ACTGG(1/-1)
BstYI 587 GGATCC [GA]^GATC[CT]
DraII 535 GGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 410 TGGCCA [CT]^GGCC[GA]
EarI 598 CTCTTC CTCTTC(1/4)
EcoO109I 535 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 422 CCAAGG C^C[AT][AT]GG
EcoT22I 184 ATGCAT ATGCA^T
Fnu4HI 568 GCTGC GC^[ACGT]GC
ItaI 568 GCTGC GC^[ACGT]GC
Ksp632I 598 CTCTTC CTCTTC(1/4)
MaeI 250 CTAG C^TAG
MaeII 82 ACGT A^CGT
MflI 587 GGATCC [GA]^GATC[CT]
MluNI 410 TGGCCA TGG^CCA
MroI 517 TCCGGA T^CCGGA
MscI 410 TGGCCA TGG^CCA
NsiI 184 ATGCAT ATGCA^T
NspI 578 ACATGC [GA]CATG^[CT]
PpuMI 535 GGGTCCT [GA]G^G[AT]CC[CT]
RsaI 173 GTAC GT^AC
StyI 422 CCAAGG C^C[AT][AT]GG
TseI 568 GCTGC G^C[AT]GC
XbaI 249 TCTAGA T^CTAGA
XhoII 587 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AvaII 536 GGTCC G^G[AT]CC
771 GGACC
BsaWI 517 TCCGGA [AT]^CCGG[AT]
584 ACCGGA
BsiYI 163 CCAAAGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
624 CCCAAAGCGGG
BslI 163 CCAAAGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
624 CCCAAAGCGGG
BstXI 373 CCATCTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
761 CCAGCATTATGG
DdeI 482 CTCAG C^T[ACGT]AG
489 CTTAG
FauI 533 GCGGG CCCGC(4/6)
630 GCGGG
HaeIII 411 GGCC GG^CC
837 GGCC
HapII 518 CCGG C^CGG
585 CCGG
HpaII 518 CCGG C^CGG
585 CCGG
HphI 217 GGTGA GGTGA(8/7)
328 TCACC
MseI 53 TTAA T^TAA
614 TTAA
MspI 518 CCGG C^CGG
585 CCGG
NlaIV 535 GGGTCC GG[ACGT]^[ACGT]CC
587 GGATCC
PleI 246 GACTC GAGTC(4/5)
74 GAGTC
SfaNI 183 GATGC GCATC(5/9)
804 GATGC
TfiI 204 GATTC G^A[AT]TC
366 GATTC
Tru9I 53 TTAA T^TAA
614 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AcsI AlwI ApoI BssKI
BstF5I BstNI Cfr13I DpnII EcoRII
FokI HinfI MboI MboII MnlI
MvaI NdeII NlaIII Sau3AI Sau96I
ScrFI Tsp509I XcmI