Restriction map for
pF1KB5977
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BalI BanI BanII BbeI BbrPI
BbvCI BciVI BclI BcnI BfrI
BlnI BlpI BmyI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiHKAI BsiWI BsmBI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraIII
DrdI DsaI Eam1105I Eco47III Eco81I
EcoNI EcoO65I EcoRI FauI FbaI
FseI FspI HaeII HapII HhaI
Hin1I HinP1I HpaI HpaII KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SgrAI SmaI SmlI SnaBI SphI
Sse8387I SspBI StuI SwaI Van91I
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcsI 529 GAATTT [GA]^AATT[CT]
AfaI 1476 GTAC GT^AC
AflIII 597 ACATGT A^C[GA][CT]GT
AlwNI 820 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 529 GAATTT [GA]^AATT[CT]
AspI 868 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 75 GGACC G^G[AT]CC
BamHI 762 GGATCC G^GATCC
BcgI 1228 CGAATGAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 472 GCCTATATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 213 AGATCT A^GATCT
BsiEI 5 CGGCCG CG[GA][CT]^CG
BsmFI 32 GGGAC GGGAC(10/14)
BspLU11I 597 ACATGT A^CATGT
BssKI 380 CCAGG ^CC[ACGT]GG
BstBI 1412 TTCGAA TT^CGAA
BstNI 380 CCAGG CC^[AT]GG
DraI 1456 TTTAAA TTT^AAA
DraII 74 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 5 CGGCCG [CT]^GGCC[GA]
EagI 5 CGGCCG C^GGCCG
EarI 385 GAAGAG CTCTTC(1/4)
EclXI 5 CGGCCG C^GGCCG
Eco52I 5 CGGCCG C^GGCCG
EcoO109I 74 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRII 380 CCAGG ^CC[AT]GG
EcoRV 1398 GATATC GAT^ATC
EcoT22I 908 ATGCAT ATGCA^T
HgaI 868 GACGC GACGC(5/10)
HincII 874 GTCAAC GT[CT]^[GA]AC
HindII 874 GTCAAC GT[CT]^[GA]AC
HindIII 1142 AAGCTT A^AGCTT
Ksp632I 385 GAAGAG CTCTTC(1/4)
MaeII 1281 ACGT A^CGT
MslI 970 CATGTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 380 CCAGG CC^[AT]GG
NsiI 908 ATGCAT ATGCA^T
NspI 597 ACATGT [GA]CATG^[CT]
NspV 1412 TTCGAA TT^CGAA
PflFI 868 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 74 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 648 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 1476 GTAC GT^AC
ScaI 1475 AGTACT AGT^ACT
ScrFI 380 CCAGG CC^[ACGT]GG
SfuI 1412 TTCGAA TT^CGAA
SpeI 1488 ACTAGT A^CTAGT
Tth111I 868 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1081 TCTAGA T^CTAGA
XcmI 650 CCAAGGTCAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 25 CCGC CCGC(-3/-1)
4 GCGG
BbsI 670 GAAGAC GAAGAC(2/6)
137 GTCTTC
BpuAI 670 GAAGAC GAAGAC(2/6)
137 GTCTTC
BseRI 20 CTCCTC GAGGAG(10/8)
1111 GAGGAG
BsmI 468 GAATGC GAATGC(1/-1)
1528 GCATTC
BsrDI 324 CATTGC GCAATG(2/0)
1551 CATTGC
BsrI 1520 ACTGG ACTGG(1/-1)
1125 CCAGT
Cfr13I 75 GGACC G^G[ACGT]CC
172 GGCCC
EcoT14I 650 CCAAGG C^C[AT][AT]GG
986 CCTTGG
HaeIII 6 GGCC GG^CC
172 GGCC
PleI 673 GACTC GAGTC(4/5)
106 GAGTC
PvuII 1022 CAGCTG CAG^CTG
1507 CAGCTG
Sau96I 75 GGACC G^G[ACGT]CC
172 GGCCC
SfaNI 907 GATGC GCATC(5/9)
1255 GATGC
SspI 590 AATATT AAT^ATT
840 AATATT
StyI 650 CCAAGG C^C[AT][AT]GG
986 CCTTGG
TaqI 1227 TCGA T^CGA
1413 TCGA
TthHB8I 1227 TCGA T^CGA
1413 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AluI AlwI BbvI BfaI BpmI
BsaJI BsiYI BslI BsmAI BstF5I
BstYI Cac8I DdeI DpnII Fnu4HI
FokI HinfI HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MwoI NdeII NlaIII
NlaIV PstI Sau3AI SfcI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII