Restriction map for
pF1KB7664
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaBI
BsaI BsgI BsiEI BsiHKAI BsiWI
BsmI BspDI BspHI BspLU11I BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I CelII CfoI
ClaI CpoI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HaeII HhaI
HinP1I HpaI KasI KpnI MamI
MflI MluI MluNI MscI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SspI SwaI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 851 GTCGAC GT^[AC][GT]AC
AccII 196 CGCG CG^CG
AccIII 135 TCCGGA T^CCGGA
AcsI 1075 AAATTT [GA]^AATT[CT]
AcyI 776 GGCGTC G[GA]^CG[CT]C
AlwNI 508 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 251 CCTCAGG CC^T[ACGT]AGG
ApoI 1075 AAATTT [GA]^AATT[CT]
BanI 651 GGTGCC G^G[CT][GA]CC
BbsI 41 GAAGAC GAAGAC(2/6)
BciVI 758 GTATCC GTATCC(6/5)
BclI 882 TGATCA T^GATCA
BfaI 404 CTAG C^TAG
BpuAI 41 GAAGAC GAAGAC(2/6)
BsaAI 755 CACGTA [CT]AC^GT[GA]
BsaHI 776 GGCGTC G[GA]^CG[CT]C
BseAI 135 TCCGGA T^CCGGA
Bsp1407I 818 TGTACA T^GTACA
BspEI 135 TCCGGA T^CCGGA
BspMI 512 ACCTGC ACCTGC(4/8)
BsrGI 818 TGTACA T^GTACA
BstUI 196 CGCG CG^CG
BstXI 141 CCACTCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 251 CCTCAGG CC^T[ACGT]AGG
DraI 790 TTTAAA TTT^AAA
DsaI 195 CCGCGG C^C[GA][CT]GG
EaeI 960 TGGCCG [CT]^GGCC[GA]
Eco81I 251 CCTCAGG CC^T[ACGT]AGG
EcoT14I 990 CCTTGG C^C[AT][AT]GG
FbaI 882 TGATCA T^GATCA
Hin1I 776 GGCGTC G[GA]^CG[CT]C
HincII 851 GTCGAC GT[CT]^[GA]AC
HindII 851 GTCGAC GT[CT]^[GA]AC
HindIII 672 AAGCTT A^AGCTT
KspI 195 CCGCGG CCGC^GG
MaeI 404 CTAG C^TAG
MaeIII 413 GTGAC ^GT[ACGT]AC
MfeI 878 CAATTG C^AATTG
MroI 135 TCCGGA T^CCGGA
MslI 142 CACTCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 195 CCGCGG C[AC]G^C[GT]G
MunI 878 CAATTG C^AATTG
MvnI 196 CGCG CG^CG
NspI 906 GCATGC [GA]CATG^[CT]
PstI 514 CTGCAG CTGCA^G
SacII 195 CCGCGG CCGC^GG
SalI 851 GTCGAC G^TCGAC
SbfI 513 CCTGCAGG CCTGCA^GG
SfaNI 564 GCATC GCATC(5/9)
SfcI 514 CTGCAG C^T[GA][CT]AG
SmlI 697 CTTGAG C^T[CT][GA]AG
SphI 906 GCATGC GCATG^C
Sse8387I 513 CCTGCAGG CCTGCA^GG
SspBI 818 TGTACA T^GTACA
StyI 990 CCTTGG C^C[AT][AT]GG
TfiI 1082 GATTC G^A[AT]TC
Tsp45I 413 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AluI 402 AGCT AG^CT
673 AGCT
AvaI 186 CTCGGG C^[CT]CG[GA]G
209 CCCGAG
AvaII 138 GGACC G^G[AT]CC
224 GGTCC
BanII 206 GAGCCC G[GA]GC[CT]^C
742 GAGCCC
BsaWI 135 TCCGGA [AT]^CCGG[AT]
145 TCCGGT
BsoBI 186 CTCGGG C^[CT]CG[GA]G
209 CCCGAG
BsrBI 183 CCGCTC CCGCTC(-3/-3)
532 GAGCGG
BsrDI 864 CATTGC GCAATG(2/0)
1001 GCAATG
BsrI 846 ACTGG ACTGG(1/-1)
811 CCAGT
HgaI 96 GCGTC GACGC(5/10)
777 GCGTC
PleI 601 GAGTC GAGTC(4/5)
1114 GAGTC
SapI 12 GAAGAGC GCTCTTC(1/4)
529 GAAGAGC
StuI 248 AGGCCT AGG^CCT
466 AGGCCT
Tsp509I 879 AATT ^AATT
1076 AATT
TspRI 411 CAGTG CA[GC]TG[ACGT][ACGT]^
597 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AlwI BbvI BcnI
BmyI BsaJI BseRI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BsrFI
BssKI BstF5I BstNI Cac8I Cfr10I
Cfr13I DdeI DpnII EarI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeII MboI MboII MnlI MseI
MspI MvaI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI TaqI Tru9I
TseI TthHB8I