Restriction map for
pF1KB3929
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BalI
BamHI BbrPI BbsI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BsgI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII Eam1105I Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FspI HindIII HpaI KpnI
MaeII MaeIII MamI MfeI MflI
MluI MluNI MroI MscI MslI
MunI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SalI SapI
SbfI SexAI SfcI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tsp45I TspRI Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AlwNI 518 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 100 GGGCCC GGGCC^C
AspHI 238 GAGCTC G[AT]GC[AT]^C
AvaI 83 CCCGAG C^[CT]CG[GA]G
BbeI 55 GGCGCC GGCGC^C
BbvCI 157 GCTGAGG CCTCAGC(-5/-2)
BbvI 123 GCAGC GCAGC(8/12)
BcnI 59 CCCGG CC^[GC]GG
BfaI 616 CTAG C^TAG
BglI 58 GCCCGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 262 CTGGAG CTGGAG(16/14)
BsiHKAI 238 GAGCTC G[AT]GC[AT]^C
BsoBI 83 CCCGAG C^[CT]CG[GA]G
BspMI 182 GCAGGT ACCTGC(4/8)
BstAPI 126 GCAGTACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 629 CCATGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 635 GACTGGGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 228 CTCTTC CTCTTC(1/4)
FauI 112 CCCGC CCCGC(4/6)
FseI 62 GGCCGGCC GGCCGG^CC
HaeII 55 GGCGCC [GA]GCGC^[CT]
HincII 736 GTCAAC GT[CT]^[GA]AC
HindII 736 GTCAAC GT[CT]^[GA]AC
KasI 55 GGCGCC G^GCGCC
Ksp632I 228 CTCTTC CTCTTC(1/4)
KspI 113 CCGCGG CCGC^GG
MaeI 616 CTAG C^TAG
MseI 335 TTAA T^TAA
MspA1I 113 CCGCGG C[AC]G^C[GT]G
NaeI 63 GCCGGC GCC^GGC
NarI 55 GGCGCC GG^CGCC
NciI 59 CCCGG CC^[GC]GG
NcoI 629 CCATGG C^CATGG
NgoMIV 63 GCCGGC G^CCGGC
SacI 238 GAGCTC GAGCT^C
SacII 113 CCGCGG CCGC^GG
SfoI 55 GGCGCC GGC^GCC
Tru9I 335 TTAA T^TAA
TseI 123 GCAGC G^C[AT]GC
XcmI 179 CCAGCAGGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AccII 108 CGCG CG^CG
114 CGCG
AcyI 55 GGCGCC G[GA]^CG[CT]C
79 GACGCC
AspI 520 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
635 GACTGGGTC
BanI 55 GGCGCC G^G[CT][GA]CC
406 GGCACC
BanII 100 GGGCCC G[GA]GC[CT]^C
238 GAGCTC
BmyI 100 GGGCCC G[AGT]GC[ACT]^C
238 GAGCTC
BsaHI 55 GGCGCC G[GA]^CG[CT]C
79 GACGCC
BsaI 86 GAGACC GGTCTC(1/5)
385 GAGACC
BsiEI 61 CGGCCG CG[GA][CT]^CG
167 CGGCCG
BsmAI 86 GAGAC GTCTC(1/5)
385 GAGAC
Bsp1286I 100 GGGCCC G[AGT]GC[ACT]^C
238 GAGCTC
BsrFI 63 GCCGGC [GA]^CCGG[CT]
704 ACCGGC
BsrI 636 ACTGG ACTGG(1/-1)
379 CCAGT
BstF5I 510 GGATG GGATG(2/0)
665 GGATG
BstUI 108 CGCG CG^CG
114 CGCG
Cfr10I 63 GCCGGC [GA]^CCGG[CT]
704 ACCGGC
DdeI 158 CTGAG C^T[ACGT]AG
499 CTTAG
DpnII 309 GATC ^GATC
603 GATC
DraII 143 AGGTCCT [GA]G^G[ACGT]CC[CT]
184 AGGTCCC
EaeI 61 CGGCCG [CT]^GGCC[GA]
167 CGGCCG
EagI 61 CGGCCG C^GGCCG
167 CGGCCG
EclXI 61 CGGCCG C^GGCCG
167 CGGCCG
Eco52I 61 CGGCCG C^GGCCG
167 CGGCCG
EcoO109I 143 AGGTCCT [GA]G^G[ACGT]CC[CT]
184 AGGTCCC
EcoT14I 70 CCTTGG C^C[AT][AT]GG
629 CCATGG
FokI 510 GGATG GGATG(9/13)
665 GGATG
HgaI 79 GACGC GACGC(5/10)
106 GACGC
Hin1I 55 GGCGCC G[GA]^CG[CT]C
79 GACGCC
HinfI 205 GAGTC G^A[ACGT]TC
257 GAGTC
HphI 413 TCACC GGTGA(8/7)
594 TCACC
MboI 309 GATC ^GATC
603 GATC
MvnI 108 CGCG CG^CG
114 CGCG
NdeII 309 GATC ^GATC
603 GATC
PflFI 520 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
635 GACTGGGTC
PleI 205 GAGTC GAGTC(4/5)
257 GAGTC
PpuMI 143 AGGTCCT [GA]G^G[AT]CC[CT]
184 AGGTCCC
Sau3AI 309 GATC ^GATC
603 GATC
ScaI 128 AGTACT AGT^ACT
553 AGTACT
SfaNI 559 GATGC GCATC(5/9)
666 GATGC
StyI 70 CCTTGG C^C[AT][AT]GG
629 CCATGG
Tsp509I 361 AATT ^AATT
678 AATT
Tth111I 520 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
635 GACTGGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AfaI AluI AvaII BsaJI
BseRI BsiYI BslI BsmFI BssKI
BstNI Cac8I CfoI Cfr13I DsaI
EcoRII Fnu4HI HaeIII HapII HhaI
HinP1I HpaII ItaI MboII MnlI
MspI MvaI MwoI NlaIII NlaIV
RsaI Sau96I ScrFI TaqI TthHB8I