Restriction map for
pF1KB8936
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AgeI AhdI
AlwI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DdeI DraI DraII
Eam1105I EarI Eco47III Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI Hin1I HindIII
HpaI HphI KasI KpnI Ksp632I
KspI MaeIII MamI MfeI MflI
MroI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tsp45I Tsp509I TspRI Tth111I XbaI
XhoII XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AflII 832 CTTAAG C^TTAAG
AlwNI 754 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 639 GGGCCC GGGCC^C
BalI 800 TGGCCA TGG^CCA
BanI 91 GGTGCC G^G[CT][GA]CC
BfaI 769 CTAG C^TAG
BfrI 832 CTTAAG C^TTAAG
BsmAI 245 GTCTC GTCTC(1/5)
BsmBI 244 CGTCTC CGTCTC(1/5)
BsmI 458 GCATTC GAATGC(1/-1)
BspMI 818 ACCTGC ACCTGC(4/8)
BsrI 272 CCAGT ACTGG(1/-1)
BssHII 553 GCGCGC G^CGCGC
BssSI 424 CTCGTG CACGAG(-5/-1)
DraIII 151 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 236 GACATCTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 602 CGGCCG C^GGCCG
EclXI 602 CGGCCG C^GGCCG
Eco52I 602 CGGCCG C^GGCCG
HincII 313 GTCAAC GT[CT]^[GA]AC
HindII 313 GTCAAC GT[CT]^[GA]AC
MaeI 769 CTAG C^TAG
MluI 132 ACGCGT A^CGCGT
MluNI 800 TGGCCA TGG^CCA
MscI 800 TGGCCA TGG^CCA
MseI 833 TTAA T^TAA
MslI 99 CATCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 488 GCCGGC GCC^GGC
NgoMIV 488 GCCGGC G^CCGGC
NspI 608 ACATGC [GA]CATG^[CT]
PflMI 507 CCATCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 751 CTGCAG CTGCA^G
PvuII 662 CAGCTG CAG^CTG
TfiI 25 GATTC G^A[AT]TC
Tru9I 833 TTAA T^TAA
Van91I 507 CCATCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 132 ACGCGT A^C[GA][CT]GT
692 ACGTGT
AviII 440 TGCGCA TGC^GCA
666 TGCGCA
BbsI 193 GTCTTC GAAGAC(2/6)
334 GTCTTC
BpuAI 193 GTCTTC GAAGAC(2/6)
334 GTCTTC
BseRI 421 CTCCTC GAGGAG(10/8)
211 GAGGAG
BsiEI 548 CGACCG CG[GA][CT]^CG
602 CGGCCG
BsrFI 488 GCCGGC [GA]^CCGG[CT]
794 ACCGGC
BstF5I 99 CATCC GGATG(2/0)
746 CATCC
Cfr10I 488 GCCGGC [GA]^CCGG[CT]
794 ACCGGC
DpnII 207 GATC ^GATC
307 GATC
DsaI 20 CCGTGG C^C[GA][CT]GG
150 CCACGG
FokI 99 CATCC GGATG(9/13)
746 CATCC
FspI 440 TGCGCA TGC^GCA
666 TGCGCA
HaeII 50 AGCGCC [GA]GCGC^[CT]
646 AGCGCC
HgaI 120 GACGC GACGC(5/10)
243 GCGTC
MaeII 536 ACGT A^CGT
692 ACGT
MboI 207 GATC ^GATC
307 GATC
MboII 194 TCTTC GAAGA(8/7)
335 TCTTC
NdeII 207 GATC ^GATC
307 GATC
PaeR7I 46 CTCGAG C^TCGAG
730 CTCGAG
PleI 44 GACTC GAGTC(4/5)
756 GACTC
Sau3AI 207 GATC ^GATC
307 GATC
SfcI 501 CTACAG C^T[GA][CT]AG
751 CTGCAG
XcmI 95 CCAACATCCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
726 CCAGCTCGAGTATGG
XhoI 46 CTCGAG C^TCGAG
730 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AluI Alw44I ApaLI
AspHI AvaI AvaII BanII BbvI
BcnI BglI BmyI BsaJI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BssKI BstNI BstUI Cac8I CfoI
Cfr13I EaeI EcoRII FauI Fnu4HI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV Sau96I ScrFI SmaI SmlI
TaqI TseI TthHB8I XmaCI XmaI