Restriction map for
pF1KE1063
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BsaAI BsaBI
BsaHI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1286I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT14I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HpaI KasI KpnI Ksp632I
KspI MamI MfeI MflI MluI
MroI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StyI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AflIII 35 ACGTGT A^C[GA][CT]GT
BalI 2 TGGCCA TGG^CCA
BbsI 1257 GAAGAC GAAGAC(2/6)
BcnI 219 CCCGG CC^[GC]GG
BfaI 148 CTAG C^TAG
BpmI 55 CTGGAG CTGGAG(16/14)
BpuAI 1257 GAAGAC GAAGAC(2/6)
BsaI 1482 GAGACC GGTCTC(1/5)
BsaWI 739 ACCGGA [AT]^CCGG[AT]
BseRI 58 GAGGAG GAGGAG(10/8)
BstXI 250 CCAGAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 468 TTTAAA TTT^AAA
DraII 214 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 127 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 137 CCGTGG C^C[GA][CT]GG
EaeI 2 TGGCCA [CT]^GGCC[GA]
EcoNI 1514 CCTTCAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 214 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 1384 ATGCAT ATGCA^T
HindIII 1176 AAGCTT A^AGCTT
MaeI 148 CTAG C^TAG
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MwoI 565 GCTCAACCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 219 CCCGG CC^[GC]GG
NsiI 1384 ATGCAT ATGCA^T
PpuMI 214 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 931 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 572 CTGCAG CTGCA^G
SmlI 112 CTTGAG C^T[CT][GA]AG
SspI 434 AATATT AAT^ATT
StuI 1595 AGGCCT AGG^CCT
TfiI 16 GAATC G^A[AT]TC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 67 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
1665 CAGAGACTG
BsgI 233 GTGCAG GTGCAG(16/14)
1074 GTGCAG
BsmAI 1482 GAGAC GTCTC(1/5)
1667 GAGAC
Bsp1407I 720 TGTACA T^GTACA
1371 TGTACA
BspMI 570 ACCTGC ACCTGC(4/8)
235 GCAGGT
BsrGI 720 TGTACA T^GTACA
1371 TGTACA
Cac8I 315 GCAAGC GC[ACGT]^[ACGT]GC
624 GCAAGC
DpnII 168 GATC ^GATC
280 GATC
HapII 220 CCGG C^CGG
740 CCGG
HpaII 220 CCGG C^CGG
740 CCGG
MaeII 35 ACGT A^CGT
1027 ACGT
MboI 168 GATC ^GATC
280 GATC
MslI 920 CATTTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1054 CACACAAGTG
MspI 220 CCGG C^CGG
740 CCGG
NdeII 168 GATC ^GATC
280 GATC
Sau3AI 168 GATC ^GATC
280 GATC
SexAI 128 ACCTGGT A^CC[AT]GGT
183 ACCAGGT
SfaNI 144 GCATC GCATC(5/9)
1626 GCATC
SspBI 720 TGTACA T^GTACA
1371 TGTACA
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# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
AvaII BbvI BsaJI BsiYI BslI
BsmFI BsmI BsrI BssKI BstF5I
BstNI Cfr13I DdeI EcoRI EcoRII
Fnu4HI FokI HaeIII HincII HindII
HinfI HphI ItaI MaeIII MboII
MnlI MseI MvaI NlaIII NlaIV
PleI RsaI Sau96I ScrFI SfcI
Tru9I TseI Tsp45I Tsp509I TspRI
XmnI