Restriction map for
pF1KB5988
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BanI BbeI
BbrPI BbvCI BcgI BclI BcnI
BfrI BlnI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsiYI BslI BsmBI BsmFI
BsmI BsoBI BspDI BspEI BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DraI DraII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HindIII HpaI
HpaII KasI KpnI KspI MaeII
MamI MluI MroI MspI MvnI
NaeI NarI NciI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PstI PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SspI SwaI
TaqI Tth111I TthHB8I Van91I XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AlwNI 1154 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1398 ATTAAT AT^TAAT
AsnI 1398 ATTAAT AT^TAAT
AvaII 1521 GGTCC G^G[AT]CC
BalI 1016 TGGCCA TGG^CCA
BamHI 824 GGATCC G^GATCC
BbsI 183 GTCTTC GAAGAC(2/6)
BciVI 1594 GTATCC GTATCC(6/5)
BglI 1630 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 373 GCTCAGC GC^T[ACGT]AGC
BpmI 857 CTGGAG CTGGAG(16/14)
Bpu1102I 373 GCTCAGC GC^T[ACGT]AGC
BpuAI 183 GTCTTC GAAGAC(2/6)
BsmAI 1656 GAGAC GTCTC(1/5)
Bsp1407I 1455 TGTACA T^GTACA
BspHI 1611 TCATGA T^CATGA
BsrDI 522 CATTGC GCAATG(2/0)
BsrGI 1455 TGTACA T^GTACA
BstXI 135 CCAAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 373 GCTCAGC GC^T[ACGT]AGC
Cfr13I 1521 GGTCC G^G[ACGT]CC
DraIII 995 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 174 GACTCCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1016 TGGCCA [CT]^GGCC[GA]
EcoRI 245 GAATTC G^AATTC
HphI 274 TCACC GGTGA(8/7)
MluNI 1016 TGGCCA TGG^CCA
MscI 1016 TGGCCA TGG^CCA
MslI 1228 CATGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 1476 GCTAGC G^CTAGC
NlaIV 824 GGATCC GG[ACGT]^[ACGT]CC
PshAI 1256 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1398 ATTAAT AT^TAAT
RcaI 1611 TCATGA T^CATGA
SacI 715 GAGCTC GAGCT^C
SapI 529 GAAGAGC GCTCTTC(1/4)
Sau96I 1521 GGTCC G^G[ACGT]CC
SmlI 487 CTTGAG C^T[CT][GA]AG
SpeI 1351 ACTAGT A^CTAGT
SphI 987 GCATGC GCATG^C
SspBI 1455 TGTACA T^GTACA
TfiI 763 GAATC G^A[AT]TC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AflIII 957 ACATGT A^C[GA][CT]GT
1359 ACATGT
BanII 332 GGGCTC G[GA]GC[CT]^C
715 GAGCTC
BglII 732 AGATCT A^GATCT
1434 AGATCT
BsgI 283 GTGCAG GTGCAG(16/14)
1312 GTGCAG
BspLU11I 957 ACATGT A^CATGT
1359 ACATGT
DsaI 1343 CCATGG C^C[GA][CT]GG
1517 CCATGG
EarI 544 CTCTTC CTCTTC(1/4)
529 GAAGAG
EcoT14I 1343 CCATGG C^C[AT][AT]GG
1517 CCATGG
HincII 46 GTTGAC GT[CT]^[GA]AC
171 GTTGAC
HindII 46 GTTGAC GT[CT]^[GA]AC
171 GTTGAC
Ksp632I 544 CTCTTC CTCTTC(1/4)
529 GAAGAG
MfeI 869 CAATTG C^AATTG
1168 CAATTG
MunI 869 CAATTG C^AATTG
1168 CAATTG
NcoI 1343 CCATGG C^CATGG
1517 CCATGG
NdeI 345 CATATG CA^TATG
907 CATATG
SfcI 628 CTACAG C^T[GA][CT]AG
847 CTACAG
StuI 645 AGGCCT AGG^CCT
1637 AGGCCT
StyI 1343 CCATGG C^C[AT][AT]GG
1517 CCATGG
Tsp45I 692 GTGAC ^GT[GC]AC
1060 GTCAC
XbaI 1437 TCTAGA T^CTAGA
1584 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwI
ApoI AspHI BbvI BfaI BmyI
BsaJI BsiHKAI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HinfI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MspA1I MvaI MwoI NdeII NlaIII
NspI PleI PvuII RsaI Sau3AI
ScrFI SfaNI Tru9I TseI Tsp509I
TspRI XhoII