Restriction map for
pF1KB8417
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BbrPI BcgI BcnI BlnI
BlpI Bpu1102I BsaAI BsaI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsoBI BspDI BspEI BspHI BspMI
BsrBI BsrFI BssHII Bst1107I BstBI
BstEII BstF5I BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FauI FokI FseI FspI HindIII
KpnI KspI MluI MroI MslI
NaeI NciI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI Sse8387I StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccII 1678 CGCG CG^CG
AcsI 792 GAATTC [GA]^AATT[CT]
AflII 1867 CTTAAG C^TTAAG
AlwNI 1193 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 792 GAATTC [GA]^AATT[CT]
AseI 1291 ATTAAT AT^TAAT
AsnI 1291 ATTAAT AT^TAAT
BalI 59 TGGCCA TGG^CCA
BanI 3 GGCGCC G^G[CT][GA]CC
BbeI 3 GGCGCC GGCGC^C
BbvCI 1882 CCTCAGC CCTCAGC(-5/-2)
BclI 159 TGATCA T^GATCA
BfrI 1867 CTTAAG C^TTAAG
BglI 513 GCCGATATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1002 CTCCAG CTGGAG(16/14)
BsaBI 268 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 581 CTCCTC GAGGAG(10/8)
BsgI 1238 GTGCAG GTGCAG(16/14)
BsmI 1243 GAATGC GAATGC(1/-1)
Bsp1407I 1968 TGTACA T^GTACA
BsrDI 547 CATTGC GCAATG(2/0)
BsrGI 1968 TGTACA T^GTACA
BssSI 1972 CACGAG CACGAG(-5/-1)
BstUI 1678 CGCG CG^CG
BstXI 1497 CCAGATTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 753 GCATGC GC[ACGT]^[ACGT]GC
CfoI 4 GCGC GCG^C
DsaI 702 CCACGG C^C[GA][CT]GG
EaeI 59 TGGCCA [CT]^GGCC[GA]
EcoNI 1018 CCTGAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 792 GAATTC G^AATTC
FbaI 159 TGATCA T^GATCA
HaeII 3 GGCGCC [GA]GCGC^[CT]
HhaI 4 GCGC GCG^C
HinP1I 4 GCGC G^CGC
HincII 2028 GTTAAC GT[CT]^[GA]AC
HindII 2028 GTTAAC GT[CT]^[GA]AC
HpaI 2028 GTTAAC GTT^AAC
KasI 3 GGCGCC G^GCGCC
MaeII 1038 ACGT A^CGT
MamI 268 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 59 TGGCCA TGG^CCA
MscI 59 TGGCCA TGG^CCA
MvnI 1678 CGCG CG^CG
NarI 3 GGCGCC GG^CGCC
NdeI 155 CATATG CA^TATG
PleI 1338 GAGTC GAGTC(4/5)
PshBI 1291 ATTAAT AT^TAAT
PvuII 2291 CAGCTG CAG^CTG
SapI 129 GCTCTTC GCTCTTC(1/4)
ScaI 87 AGTACT AGT^ACT
SfoI 3 GGCGCC GGC^GCC
SphI 753 GCATGC GCATG^C
SspBI 1968 TGTACA T^GTACA
SspI 2097 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 3 GGCGCC G[GA]^CG[CT]C
1120 GACGCC
AflIII 246 ACATGT A^C[GA][CT]GT
1427 ACATGT
AlwI 775 GGATC GGATC(4/5)
2062 GGATC
Asp700I 901 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1063 GAAGGTGTTC
BanII 127 GGGCTC G[GA]GC[CT]^C
1787 GGGCTC
BglII 744 AGATCT A^GATCT
1976 AGATCT
BsaHI 3 GGCGCC G[GA]^CG[CT]C
1120 GACGCC
BsaWI 377 TCCGGT [AT]^CCGG[AT]
1814 TCCGGT
BspLU11I 246 ACATGT A^CATGT
1427 ACATGT
BstAPI 84 GCAAGTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1588 GCACATTCTGC
BstYI 744 AGATCT [GA]^GATC[CT]
1976 AGATCT
DraI 2184 TTTAAA TTT^AAA
2331 TTTAAA
DraII 1014 AGGTCCT [GA]G^G[ACGT]CC[CT]
1878 AGGTCCT
EarI 130 CTCTTC CTCTTC(1/4)
916 GAAGAG
EcoO109I 1014 AGGTCCT [GA]G^G[ACGT]CC[CT]
1878 AGGTCCT
HapII 378 CCGG C^CGG
1815 CCGG
HgaI 1120 GACGC GACGC(5/10)
1872 GCGTC
Hin1I 3 GGCGCC G[GA]^CG[CT]C
1120 GACGCC
HpaII 378 CCGG C^CGG
1815 CCGG
Ksp632I 130 CTCTTC CTCTTC(1/4)
916 GAAGAG
MfeI 797 CAATTG C^AATTG
1998 CAATTG
MflI 744 AGATCT [GA]^GATC[CT]
1976 AGATCT
MspA1I 2254 CCGCTG C[AC]G^C[GT]G
2291 CAGCTG
MspI 378 CCGG C^CGG
1815 CCGG
MunI 797 CAATTG C^AATTG
1998 CAATTG
PpuMI 1014 AGGTCCT [GA]G^G[AT]CC[CT]
1878 AGGTCCT
SpeI 1138 ACTAGT A^CTAGT
2169 ACTAGT
XhoII 744 AGATCT [GA]^GATC[CT]
1976 AGATCT
XmnI 901 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1063 GAAGGTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI AvaII BbsI
BbvI BciVI BfaI BmyI BpuAI
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstNI Cfr13I DdeI
DpnII EcoRII EcoT14I Fnu4HI HaeIII
HinfI HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV NspI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I