Restriction map for
pF1KB9546
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BcgI BciVI
BclI BfrI BglII BlnI BsaAI
BsaBI BsaWI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssSI Bst1107I BstBI
BstXI BstYI BstZ17I ClaI CpoI
DraI DraIII Eam1105I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HpaI KpnI MaeII MamI
MfeI MflI MluI MroI MunI
NdeI NheI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tsp509I Tth111I TthHB8I XbaI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Alw44I 1120 GTGCAC G^TGCAC
AlwI 95 GGATC GGATC(4/5)
Aor51HI 723 AGCGCT AGC^GCT
ApaLI 1120 GTGCAC G^TGCAC
BalI 1112 TGGCCA TGG^CCA
BbeI 1029 GGCGCC GGCGC^C
BbvCI 999 CCTCAGC CCTCAGC(-5/-2)
BsaI 826 GAGACC GGTCTC(1/5)
BsiEI 619 CGGCCG CG[GA][CT]^CG
BsrDI 473 GCAATG GCAATG(2/0)
BssHII 43 GCGCGC G^CGCGC
BstAPI 1164 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 556 GACAACAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 619 CGGCCG C^GGCCG
EclXI 619 CGGCCG C^GGCCG
Eco47III 723 AGCGCT AGC^GCT
Eco52I 619 CGGCCG C^GGCCG
EcoNI 170 CCTTGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII 119 AAGCTT A^AGCTT
KasI 1029 GGCGCC G^GCGCC
KspI 91 CCGCGG CCGC^GG
MluNI 1112 TGGCCA TGG^CCA
MscI 1112 TGGCCA TGG^CCA
MseI 1075 TTAA T^TAA
NaeI 14 GCCGGC GCC^GGC
NarI 1029 GGCGCC GG^CGCC
NcoI 485 CCATGG C^CATGG
NgoMIV 14 GCCGGC G^CCGGC
NotI 618 GCGGCCGC GC^GGCCGC
PflMI 1206 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 647 GAGCTC GAGCT^C
SacII 91 CCGCGG CCGC^GG
SfoI 1029 GGCGCC GGC^GCC
TfiI 231 GATTC G^A[AT]TC
Tru9I 1075 TTAA T^TAA
Van91I 1206 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 37 enzymes) #
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AcyI 1029 GGCGCC G[GA]^CG[CT]C
1236 GGCGTC
AocI 157 CCTCAGG CC^T[ACGT]AGG
208 CCTCAGG
ApaI 1322 GGGCCC GGGCC^C
1402 GGGCCC
BbsI 673 GAAGAC GAAGAC(2/6)
1266 GAAGAC
BfaI 727 CTAG C^TAG
1379 CTAG
BglI 481 GCCGCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1286 GCCAGAGGGGC
BlpI 105 GCTCAGC GC^T[ACGT]AGC
426 GCTGAGC
Bpu1102I 105 GCTCAGC GC^T[ACGT]AGC
426 GCTGAGC
BpuAI 673 GAAGAC GAAGAC(2/6)
1266 GAAGAC
BsaHI 1029 GGCGCC G[GA]^CG[CT]C
1236 GGCGTC
BsgI 586 GTGCAG GTGCAG(16/14)
1246 GTGCAG
BsmAI 826 GAGAC GTCTC(1/5)
1332 GAGAC
BsmFI 263 GGGAC GGGAC(10/14)
1223 GGGAC
BspMI 962 ACCTGC ACCTGC(4/8)
979 ACCTGC
BsrI 1102 ACTGG ACTGG(1/-1)
1299 CCAGT
BstEII 735 GGTTACC G^GT[ACGT]ACC
1362 GGTCACC
BstF5I 1304 CATCC GGATG(2/0)
1106 GGATG
BstPI 735 GGTTACC G^GT[ACGT]ACC
1362 GGTCACC
Bsu36I 157 CCTCAGG CC^T[ACGT]AGG
208 CCTCAGG
CelII 105 GCTCAGC GC^T[ACGT]AGC
426 GCTGAGC
EarI 783 GAAGAG CTCTTC(1/4)
1153 GAAGAG
Eco81I 157 CCTCAGG CC^T[ACGT]AGG
208 CCTCAGG
EcoO65I 735 GGTTACC G^GT[ACGT]ACC
1362 GGTCACC
EcoT14I 485 CCATGG C^C[AT][AT]GG
1034 CCAAGG
FokI 1304 CATCC GGATG(9/13)
1106 GGATG
Hin1I 1029 GGCGCC G[GA]^CG[CT]C
1236 GGCGTC
Ksp632I 783 GAAGAG CTCTTC(1/4)
1153 GAAGAG
MaeI 727 CTAG C^TAG
1379 CTAG
PleI 225 GACTC GAGTC(4/5)
613 GAGTC
PpuMI 153 GGGTCCT [GA]G^G[AT]CC[CT]
896 AGGACCC
SfaNI 1107 GATGC GCATC(5/9)
524 GCATC
SmaI 255 CCCGGG CCC^GGG
984 CCCGGG
StuI 729 AGGCCT AGG^CCT
1070 AGGCCT
StyI 485 CCATGG C^C[AT][AT]GG
1034 CCAAGG
XcmI 740 CCACGGAACATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1412 CCAAGCCCTCAGTGG
XmaCI 255 CCCGGG C^CCGGG
984 CCCGGG
XmaI 255 CCCGGG C^CCGGG
984 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AluI AlwNI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsoBI Bsp1286I
BsrBI BsrFI BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PstI PvuII Sau3AI
Sau96I ScrFI SfcI SmlI TseI
Tsp45I TspRI