Restriction map for
pF1KB6788
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BciVI BclI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiWI BsmFI BsmI BsoBI Bsp1407I
BspEI BspHI BspLU11I BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I Cfr13I CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HinfI HpaI KasI KpnI Ksp632I
KspI MaeII MaeIII MamI MfeI
MflI MluI MluNI MroI MscI
MseI MslI MunI NarI NcoI
NdeI NheI NlaIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 349 GAATTC [GA]^AATT[CT]
ApoI 349 GAATTC [GA]^AATT[CT]
AspHI 157 GAGCAC G[AT]GC[AT]^C
BcgI 363 GCACAACATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 354 CCGGG CC^[GC]GG
BfaI 166 CTAG C^TAG
BlpI 440 GCTCAGC GC^T[ACGT]AGC
BmyI 157 GAGCAC G[AGT]GC[ACT]^C
BpmI 446 CTGGAG CTGGAG(16/14)
Bpu1102I 440 GCTCAGC GC^T[ACGT]AGC
BsaI 456 GGTCTC GGTCTC(1/5)
BsaJI 404 CCAAGG C^C[ACGT][ACGT]GG
BseRI 224 GAGGAG GAGGAG(10/8)
BsiEI 15 CGGTCG CG[GA][CT]^CG
BsiHKAI 157 GAGCAC G[AT]GC[AT]^C
BsiYI 96 CCTTCGGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 96 CCTTCGGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 373 CGTCTC CGTCTC(1/5)
Bsp1286I 157 GAGCAC G[AGT]GC[ACT]^C
BspDI 172 ATCGAT AT^CGAT
BspMI 72 GCAGGT ACCTGC(4/8)
BsrBI 39 GAGCGG CCGCTC(-3/-3)
BsrFI 22 GCCGGC [GA]^CCGG[CT]
BstF5I 122 CATCC GGATG(2/0)
BstNI 117 CCTGG CC^[AT]GG
CelII 440 GCTCAGC GC^T[ACGT]AGC
Cfr10I 22 GCCGGC [GA]^CCGG[CT]
ClaI 172 ATCGAT AT^CGAT
EcoNI 96 CCTTCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 349 GAATTC G^AATTC
EcoRII 117 CCTGG ^CC[AT]GG
EcoT14I 404 CCAAGG C^C[AT][AT]GG
FokI 122 CATCC GGATG(9/13)
HphI 75 GGTGA GGTGA(8/7)
MaeI 166 CTAG C^TAG
MvaI 117 CCTGG CC^[AT]GG
NaeI 22 GCCGGC GCC^GGC
NciI 354 CCGGG CC^[GC]GG
NgoMIV 22 GCCGGC G^CCGGC
SfaNI 121 GCATC GCATC(5/9)
StuI 114 AGGCCT AGG^CCT
StyI 404 CCAAGG C^C[AT][AT]GG
TaqI 173 TCGA T^CGA
Tsp509I 350 AATT ^AATT
TthHB8I 173 TCGA T^CGA
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccII 19 CGCG CG^CG
45 CGCG
BssKI 117 CCTGG ^CC[ACGT]GG
354 CCGGG
BstUI 19 CGCG CG^CG
45 CGCG
CfoI 20 GCGC GCG^C
44 GCGC
DpnII 171 GATC ^GATC
297 GATC
HaeIII 115 GGCC GG^CC
435 GGCC
HapII 23 CCGG C^CGG
354 CCGG
HhaI 20 GCGC GCG^C
44 GCGC
HinP1I 20 GCGC G^CGC
44 GCGC
HpaII 23 CCGG C^CGG
354 CCGG
MboI 171 GATC ^GATC
297 GATC
MboII 294 GAAGA GAAGA(8/7)
385 GAAGA
MspI 23 CCGG C^CGG
354 CCGG
MvnI 19 CGCG CG^CG
45 CGCG
NdeII 171 GATC ^GATC
297 GATC
NlaIII 216 CATG CATG^
378 CATG
PvuII 131 CAGCTG CAG^CTG
443 CAGCTG
Sau3AI 171 GATC ^GATC
297 GATC
ScrFI 117 CCTGG CC^[ACGT]GG
354 CCGGG
TspRI 179 CACTG CA[GC]TG[ACGT][ACGT]^
308 CACTG
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# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
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AciI AluI BbvI BsmAI Cac8I
DdeI FauI Fnu4HI ItaI MnlI
MspA1I MwoI TseI