Restriction map for
pF1KB0906
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BbsI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsiWI BsmBI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI DrdI Eam1105I
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HgaI HincII HindII
HindIII HpaI KpnI MamI MfeI
MluI MseI MslI MunI NcoI
NdeI NheI NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SalI SbfI ScaI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I TspRI Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccIII 869 TCCGGA T^CCGGA
AcsI 481 GAATTT [GA]^AATT[CT]
AcyI 93 GGCGCC G[GA]^CG[CT]C
AfaI 583 GTAC GT^AC
Alw44I 139 GTGCAC G^TGCAC
AocI 33 CCTCAGG CC^T[ACGT]AGG
ApaLI 139 GTGCAC G^TGCAC
ApoI 481 GAATTT [GA]^AATT[CT]
AviII 56 TGCGCA TGC^GCA
BalI 194 TGGCCA TGG^CCA
BbeI 93 GGCGCC GGCGC^C
BbrPI 487 CACGTG CAC^GTG
BbvCI 529 GCTGAGG CCTCAGC(-5/-2)
BfaI 538 CTAG C^TAG
BsaAI 487 CACGTG [CT]AC^GT[GA]
BsaHI 93 GGCGCC G[GA]^CG[CT]C
BsaI 166 GAGACC GGTCTC(1/5)
BsaWI 869 TCCGGA [AT]^CCGG[AT]
BseAI 869 TCCGGA T^CCGGA
BsmI 371 GCATTC GAATGC(1/-1)
BspEI 869 TCCGGA T^CCGGA
BspMI 169 ACCTGC ACCTGC(4/8)
BsrI 576 ACTGG ACTGG(1/-1)
BssHII 75 GCGCGC G^CGCGC
BstAPI 492 GCAGCGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 353 CCAAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 33 CCTCAGG CC^T[ACGT]AGG
DraIII 202 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 33 CCTCAGG CC^T[ACGT]AGG
EcoNI 108 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 473 CCTTGG C^C[AT][AT]GG
FspI 56 TGCGCA TGC^GCA
Hin1I 93 GGCGCC G[GA]^CG[CT]C
KasI 93 GGCGCC G^GCGCC
KspI 221 CCGCGG CCGC^GG
MaeI 538 CTAG C^TAG
MaeII 488 ACGT A^CGT
MluNI 194 TGGCCA TGG^CCA
MroI 869 TCCGGA T^CCGGA
MscI 194 TGGCCA TGG^CCA
NaeI 96 GCCGGC GCC^GGC
NarI 93 GGCGCC GG^CGCC
NgoMIV 96 GCCGGC G^CCGGC
PmaCI 487 CACGTG CAC^GTG
PmlI 487 CACGTG CAC^GTG
RsaI 583 GTAC GT^AC
SacII 221 CCGCGG CCGC^GG
SexAI 329 ACCAGGT A^CC[AT]GGT
SfaNI 905 GCATC GCATC(5/9)
SfoI 93 GGCGCC GGC^GCC
SmaI 89 CCCGGG CCC^GGG
StyI 473 CCTTGG C^C[AT][AT]GG
TaqI 16 TCGA T^CGA
TfiI 10 GATTC G^A[AT]TC
Tsp509I 482 AATT ^AATT
TthHB8I 16 TCGA T^CGA
XcmI 197 CCAACCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 89 CCCGGG C^CCGGG
XmaI 89 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AspHI 139 GTGCAC G[AT]GC[AT]^C
246 GTGCTC
AvaI 43 CTCGGG C^[CT]CG[GA]G
89 CCCGGG
BamHI 266 GGATCC G^GATCC
866 GGATCC
BanII 40 GGGCTC G[GA]GC[CT]^C
546 GGGCTC
BsgI 829 CTGCAC GTGCAG(16/14)
490 GTGCAG
BsiEI 98 CGGCCG CG[GA][CT]^CG
122 CGGCCG
BsiHKAI 139 GTGCAC G[AT]GC[AT]^C
246 GTGCTC
BsoBI 43 CTCGGG C^[CT]CG[GA]G
89 CCCGGG
BsrBI 175 GAGCGG CCGCTC(-3/-3)
568 GAGCGG
BsrFI 69 GCCGGT [GA]^CCGG[CT]
96 GCCGGC
BstYI 266 GGATCC [GA]^GATC[CT]
866 GGATCC
Cfr10I 69 GCCGGT [GA]^CCGG[CT]
96 GCCGGC
EagI 98 CGGCCG C^GGCCG
122 CGGCCG
EarI 856 CTCTTC CTCTTC(1/4)
3 GAAGAG
EclXI 98 CGGCCG C^GGCCG
122 CGGCCG
Eco52I 98 CGGCCG C^GGCCG
122 CGGCCG
HaeII 93 GGCGCC [GA]GCGC^[CT]
159 GGCGCT
Ksp632I 856 CTCTTC CTCTTC(1/4)
3 GAAGAG
MaeIII 690 GTCAC ^GT[ACGT]AC
911 GTGAC
MflI 266 GGATCC [GA]^GATC[CT]
866 GGATCC
PstI 23 CTGCAG CTGCA^G
551 CTGCAG
SapI 3 GAAGAGC GCTCTTC(1/4)
855 GCTCTTC
SfcI 23 CTGCAG C^T[GA][CT]AG
551 CTGCAG
Tsp45I 690 GTCAC ^GT[GC]AC
911 GTGAC
XhoII 266 GGATCC [GA]^GATC[CT]
866 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AvaII BanI BbvI BcnI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIV PflMI
PleI PpuMI Sau3AI Sau96I ScrFI
TseI Van91I