Restriction map for
pF1KA0347
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# NO CUT ENZYMES ( 74 enzymes) #
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AatII Acc65I AccI AclI AflII
AgeI AhdI AscI AseI AsnI
Asp718I AspEI AviII AvrII BbrPI
BbvCI BclI BfrI BglII BlnI
BsaAI BsaBI BsiEI BsiWI BsmBI
BspDI BspLU11I BssHII Bst1107I BstZ17I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco52I EcoRV FbaI FseI
FspI HincII HindII HpaI KpnI
KspI MamI MfeI MluI MunI
NaeI NgoMIV NheI NotI NruI
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI ScaI SfiI SgrAI SnaBI
SpeI SphI SwaI XbaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Alw44I 949 GTGCAC G^TGCAC
Aor51HI 1842 AGCGCT AGC^GCT
ApaLI 949 GTGCAC G^TGCAC
AspI 1762 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 7 GGATAC GTATCC(6/5)
BlpI 3197 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3197 GCTCAGC GC^T[ACGT]AGC
Bsp1407I 614 TGTACA T^GTACA
BsrBI 2935 CCGCTC CCGCTC(-3/-3)
BsrDI 1790 GCAATG GCAATG(2/0)
BsrFI 1400 ACCGGC [GA]^CCGG[CT]
BsrGI 614 TGTACA T^GTACA
BstAPI 3552 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 306 TTCGAA TT^CGAA
CelII 3197 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1400 ACCGGC [GA]^CCGG[CT]
DrdI 3430 GACAGCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1842 AGCGCT AGC^GCT
EcoRI 974 GAATTC G^AATTC
EcoT22I 789 ATGCAT ATGCA^T
HindIII 739 AAGCTT A^AGCTT
MseI 3383 TTAA T^TAA
NdeI 3665 CATATG CA^TATG
NsiI 789 ATGCAT ATGCA^T
NspI 755 GCATGT [GA]CATG^[CT]
NspV 306 TTCGAA TT^CGAA
PaeR7I 2411 CTCGAG C^TCGAG
PflFI 1762 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3283 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1381 GAGCTC GAGCT^C
SapI 3754 GAAGAGC GCTCTTC(1/4)
SbfI 3393 CCTGCAGG CCTGCA^GG
SfuI 306 TTCGAA TT^CGAA
SmaI 2894 CCCGGG CCC^GGG
Sse8387I 3393 CCTGCAGG CCTGCA^GG
SspBI 614 TGTACA T^GTACA
StuI 2765 AGGCCT AGG^CCT
Tru9I 3383 TTAA T^TAA
Tth111I 1762 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2411 CTCGAG C^TCGAG
XmaCI 2894 CCCGGG C^CCGGG
XmaI 2894 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccIII 827 TCCGGA T^CCGGA
2344 TCCGGA
AflIII 1325 ACGTGT A^C[GA][CT]GT
2504 ACACGT
BalI 404 TGGCCA TGG^CCA
1136 TGGCCA
BamHI 3399 GGATCC G^GATCC
3730 GGATCC
BbsI 3718 GAAGAC GAAGAC(2/6)
303 GTCTTC
BcgI 570 CGATATGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2018 GCAGCACCATCG
BfaI 971 CTAG C^TAG
1115 CTAG
BpuAI 3718 GAAGAC GAAGAC(2/6)
303 GTCTTC
BsaI 124 GAGACC GGTCTC(1/5)
2134 GGTCTC
BseAI 827 TCCGGA T^CCGGA
2344 TCCGGA
BspEI 827 TCCGGA T^CCGGA
2344 TCCGGA
BspHI 1590 TCATGA T^CATGA
3440 TCATGA
BstEII 487 GGTCACC G^GT[ACGT]ACC
2492 GGTCACC
BstPI 487 GGTCACC G^GT[ACGT]ACC
2492 GGTCACC
DraIII 553 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
700 CACGATGTG
EcoO65I 487 GGTCACC G^GT[ACGT]ACC
2492 GGTCACC
MaeI 971 CTAG C^TAG
1115 CTAG
MluNI 404 TGGCCA TGG^CCA
1136 TGGCCA
MroI 827 TCCGGA T^CCGGA
2344 TCCGGA
MscI 404 TGGCCA TGG^CCA
1136 TGGCCA
RcaI 1590 TCATGA T^CATGA
3440 TCATGA
SexAI 626 ACCAGGT A^CC[AT]GGT
881 ACCTGGT
SmlI 782 CTCAAG C^T[CT][GA]AG
2411 CTCGAG
SspI 3302 AATATT AAT^ATT
3652 AATATT
XcmI 44 CCAAGGAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2024 CCATCGTCCATGTGG
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# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI Asp700I AspHI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BglI BmyI BpmI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI