Restriction map for
pF1KB6394
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII AccI AccII AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AvaI
AviII AvrII BamHI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglII BlnI BpuAI
BsaAI BsaBI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BsrBI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I ClaI
CpoI DraI DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI HincII HindII
HpaI Ksp632I KspI MaeI MaeII
MamI MboII MluI MspA1I MvnI
NaeI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 67 enzymes) #
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AccIII 242 TCCGGA T^CCGGA
AcyI 33 GGCGCC G[GA]^CG[CT]C
AflIII 410 ACATGT A^C[GA][CT]GT
AlwNI 429 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 706 CCTGAGG CC^T[ACGT]AGG
AspHI 109 GAGCTC G[AT]GC[AT]^C
AvaII 855 GGACC G^G[AT]CC
BalI 59 TGGCCA TGG^CCA
BanII 109 GAGCTC G[GA]GC[CT]^C
BbeI 33 GGCGCC GGCGC^C
BglI 4 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 901 GCTAAGC GC^T[ACGT]AGC
BmyI 109 GAGCTC G[AGT]GC[ACT]^C
Bpu1102I 901 GCTAAGC GC^T[ACGT]AGC
BsaHI 33 GGCGCC G[GA]^CG[CT]C
BsaWI 242 TCCGGA [AT]^CCGG[AT]
BseAI 242 TCCGGA T^CCGGA
BsiHKAI 109 GAGCTC G[AT]GC[AT]^C
BsmI 507 GCATTC GAATGC(1/-1)
Bsp1286I 109 GAGCTC G[AGT]GC[ACT]^C
BspEI 242 TCCGGA T^CCGGA
BspHI 828 TCATGA T^CATGA
BspLU11I 410 ACATGT A^CATGT
BspMI 526 GCAGGT ACCTGC(4/8)
BsrDI 138 CATTGC GCAATG(2/0)
BsrFI 70 ACCGGC [GA]^CCGG[CT]
BssSI 215 CACGAG CACGAG(-5/-1)
BstAPI 372 GCACGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 454 GGATCT [GA]^GATC[CT]
Bsu36I 706 CCTGAGG CC^T[ACGT]AGG
CelII 901 GCTAAGC GC^T[ACGT]AGC
CfoI 34 GCGC GCG^C
Cfr10I 70 ACCGGC [GA]^CCGG[CT]
DraII 854 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 373 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 80 CCACGG C^C[GA][CT]GG
EaeI 59 TGGCCA [CT]^GGCC[GA]
Eco81I 706 CCTGAGG CC^T[ACGT]AGG
EcoO109I 854 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 842 CCAAGG C^C[AT][AT]GG
HaeII 33 GGCGCC [GA]GCGC^[CT]
HhaI 34 GCGC GCG^C
Hin1I 33 GGCGCC G[GA]^CG[CT]C
HinP1I 34 GCGC G^CGC
HindIII 654 AAGCTT A^AGCTT
KasI 33 GGCGCC G^GCGCC
MaeIII 67 GTCAC ^GT[ACGT]AC
MfeI 588 CAATTG C^AATTG
MflI 454 GGATCT [GA]^GATC[CT]
MluNI 59 TGGCCA TGG^CCA
MroI 242 TCCGGA T^CCGGA
MscI 59 TGGCCA TGG^CCA
MseI 890 TTAA T^TAA
MslI 808 CACTCGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 588 CAATTG C^AATTG
NarI 33 GGCGCC GG^CGCC
NspI 410 ACATGT [GA]CATG^[CT]
PpuMI 854 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 828 TCATGA T^CATGA
SacI 109 GAGCTC GAGCT^C
SfoI 33 GGCGCC GGC^GCC
SgrAI 69 CACCGGCG C[GA]^CCGG[CT]G
SmlI 382 CTTGAG C^T[CT][GA]AG
StyI 842 CCAAGG C^C[AT][AT]GG
Tru9I 890 TTAA T^TAA
Tsp45I 67 GTCAC ^GT[GC]AC
XhoII 454 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 11 enzymes) #
###################################
Acc65I 400 GGTACC G^GTACC
819 GGTACC
AfaI 401 GTAC GT^AC
820 GTAC
AlwI 454 GGATC GGATC(4/5)
584 GGATC
Asp718I 400 GGTACC G^GTACC
819 GGTACC
FauI 685 CCCGC CCCGC(4/6)
74 GCGGG
HinfI 218 GAGTC G^A[ACGT]TC
795 GAGTC
KpnI 400 GGTACC GGTAC^C
819 GGTACC
PleI 218 GAGTC GAGTC(4/5)
795 GAGTC
RsaI 401 GTAC GT^AC
820 GTAC
SfaNI 86 GCATC GCATC(5/9)
142 GCATC
TspRI 573 CAGTG CA[GC]TG[ACGT][ACGT]^
762 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI BanI BbvI BpmI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HapII
HpaII HphI ItaI MboI MnlI
MspI MvaI MwoI NdeII NlaIII
NlaIV PstI Sau3AI Sau96I ScrFI
SfcI TseI Tsp509I