Restriction map for
pF1KE0406
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaHI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoO65I EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeII MfeI MflI
MluI MslI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI Tth111I XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 585 TCCGGA T^CCGGA
AfaI 752 GTAC GT^AC
AflIII 341 ACATGT A^C[GA][CT]GT
AlwI 869 GGATC GGATC(4/5)
AlwNI 471 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 976 CCTCAGG CC^T[ACGT]AGG
ApaI 382 GGGCCC GGGCC^C
AvaII 880 GGTCC G^G[AT]CC
BalI 832 TGGCCA TGG^CCA
BanI 391 GGTGCC G^G[CT][GA]CC
BanII 382 GGGCCC G[GA]GC[CT]^C
BbvCI 395 CCTCAGC CCTCAGC(-5/-2)
BsaBI 523 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 27 GAGACC GGTCTC(1/5)
BsaWI 585 TCCGGA [AT]^CCGG[AT]
BseAI 585 TCCGGA T^CCGGA
BseRI 79 GAGGAG GAGGAG(10/8)
BsgI 239 GTGCAG GTGCAG(16/14)
BspEI 585 TCCGGA T^CCGGA
BspHI 794 TCATGA T^CATGA
BspLU11I 341 ACATGT A^CATGT
BspMI 400 GCAGGT ACCTGC(4/8)
Bsu36I 976 CCTCAGG CC^T[ACGT]AGG
DpnII 870 GATC ^GATC
EaeI 832 TGGCCA [CT]^GGCC[GA]
Eco81I 976 CCTCAGG CC^T[ACGT]AGG
EcoNI 33 CCTTCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 480 GAATTC G^AATTC
HapII 586 CCGG C^CGG
HpaII 586 CCGG C^CGG
MamI 523 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 870 GATC ^GATC
MluNI 832 TGGCCA TGG^CCA
MroI 585 TCCGGA T^CCGGA
MscI 832 TGGCCA TGG^CCA
MspA1I 323 CAGCTG C[AC]G^C[GT]G
MspI 586 CCGG C^CGG
NdeII 870 GATC ^GATC
NspI 341 ACATGT [GA]CATG^[CT]
PflMI 835 CCACAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 879 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 701 CTGCAG CTGCA^G
PvuII 323 CAGCTG CAG^CTG
RcaI 794 TCATGA T^CATGA
RsaI 752 GTAC GT^AC
Sau3AI 870 GATC ^GATC
SexAI 308 ACCAGGT A^CC[AT]GGT
SspI 1018 AATATT AAT^ATT
TaqI 156 TCGA T^CGA
TthHB8I 156 TCGA T^CGA
Van91I 835 CCACAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 957 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 18 enzymes) #
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BbsI 798 GAAGAC GAAGAC(2/6)
373 GTCTTC
BfaI 544 CTAG C^TAG
958 CTAG
BmyI 382 GGGCCC G[AGT]GC[ACT]^C
445 GGGCAC
BpuAI 798 GAAGAC GAAGAC(2/6)
373 GTCTTC
BsaJI 453 CCCAGG C^C[ACGT][ACGT]GG
475 CCCTGG
BsmAI 27 GAGAC GTCTC(1/5)
179 GAGAC
BsmFI 473 GTCCC GGGAC(10/14)
934 GTCCC
Bsp1286I 382 GGGCCC G[AGT]GC[ACT]^C
445 GGGCAC
BstXI 129 CCAGTATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
866 CCAGGATCATGG
Cac8I 297 GCAAGC GC[ACGT]^[ACGT]GC
830 GCTGGC
DraII 381 GGGGCCC [GA]G^G[ACGT]CC[CT]
879 AGGTCCT
EcoO109I 381 GGGGCCC [GA]G^G[ACGT]CC[CT]
879 AGGTCCT
HinfI 612 GATTC G^A[ACGT]TC
954 GATTC
HphI 42 GGTGA GGTGA(8/7)
738 GGTGA
MaeI 544 CTAG C^TAG
958 CTAG
TfiI 612 GATTC G^A[AT]TC
954 GATTC
Tsp45I 43 GTGAC ^GT[GC]AC
919 GTCAC
XcmI 203 CCATATCAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
755 CCATTAGACCAATGG
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BsiYI BslI BsrI BssKI BstF5I
BstNI Cfr13I DdeI EcoRII Fnu4HI
FokI HaeIII ItaI MaeIII MboII
MnlI MseI MvaI MwoI NlaIII
NlaIV Sau96I ScrFI SfaNI SfcI
Tru9I TseI Tsp509I TspRI