##################################
# NO CUT ENZYMES (  191 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        AluI        Alw44I      AlwNI       Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
HapII       HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       HphI        
KasI        KpnI        Ksp632I     KspI        MaeIII      
MamI        MfeI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MspI        MunI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
PstI        PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tsp45I      Tth111I     
Van91I      XbaI        XcmI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AclI          376  AACGTT               AA^CGTT
AcsI          217  AAATTC               [GA]^AATT[CT]
AocI           33  CCTGAGG              CC^T[ACGT]AGG
ApoI          217  AAATTC               [GA]^AATT[CT]
BbsI          106  GAAGAC               GAAGAC(2/6)
BbvI          331  GCAGC                GCAGC(8/12)
BfaI          259  CTAG                 C^TAG
BpuAI         106  GAAGAC               GAAGAC(2/6)
BsiYI         120  CCCTGGTGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          120  CCCTGGTGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrBI          16  GAGCGG               CCGCTC(-3/-3)
BsrDI         252  GCAATG               GCAATG(2/0)
BsrI          370  ACTGG                ACTGG(1/-1)
BstYI          29  AGATCC               [GA]^GATC[CT]
Bsu36I         33  CCTGAGG              CC^T[ACGT]AGG
Cfr13I         20  GGGCC                G^G[ACGT]CC
DrdI          109  GACCTGTCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I         33  CCTGAGG              CC^T[ACGT]AGG
EcoT14I         8  CCAAGG               C^C[AT][AT]GG
FauI           18  GCGGG                CCCGC(4/6)
Fnu4HI        331  GCAGC                GC^[ACGT]GC
HaeIII         21  GGCC                 GG^CC
HgaI          362  GCGTC                GACGC(5/10)
HinfI         340  GAGTC                G^A[ACGT]TC
ItaI          331  GCAGC                GC^[ACGT]GC
MaeI          259  CTAG                 C^TAG
MaeII         377  ACGT                 A^CGT
MflI           29  AGATCC               [GA]^GATC[CT]
NlaIII        133  CATG                 CATG^
PaeR7I        179  CTCGAG               C^TCGAG
PleI          340  GAGTC                GAGTC(4/5)
Psp1406I      376  AACGTT               AA^CGTT
Sau96I         20  GGGCC                G^G[ACGT]CC
StyI            8  CCAAGG               C^C[AT][AT]GG
TseI          331  GCAGC                G^C[AT]GC
TspRI         173  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoI          179  CTCGAG               C^TCGAG
XhoII          29  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AciI          222  CCGC                 CCGC(-3/-1)
               18  GCGG                
AlwI           30  GATCC                GGATC(4/5)
              291  GATCC               
AvaI          179  CTCGAG               C^[CT]CG[GA]G
              337  CCCGAG              
BsmFI         118  GTCCC                GGGAC(10/14)
              342  GTCCC               
BsoBI         179  CTCGAG               C^[CT]CG[GA]G
              337  CCCGAG              
DdeI           34  CTGAG                C^T[ACGT]AG
               74  CTGAG               
MseI          188  TTAA                 T^TAA
              367  TTAA                
SfaNI          70  GATGC                GCATC(5/9)
              351  GATGC               
SmlI          160  CTCAAG               C^T[CT][GA]AG
              179  CTCGAG              
Tru9I         188  TTAA                 T^TAA
              367  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   19 enzymes) #
#########################################

BsaJI       BsmAI       BssKI       BstF5I      BstNI       
Cac8I       DpnII       EcoRII      FokI        MboI        
MboII       MnlI        MvaI        NdeII       Sau3AI      
ScrFI       TaqI        Tsp509I     TthHB8I