Restriction map for
pF1KE0185
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbsI BbvCI BbvI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BmyI BpmI BpuAI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I Cac8I CfoI Cfr10I ClaI
CpoI DraII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FauI Fnu4HI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII ItaI KasI KpnI
Ksp632I KspI MfeI MflI MluI
MluNI MroI MscI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TseI Tth111I
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AfaI 736 GTAC GT^AC
AlwI 609 GGATC GGATC(4/5)
AvaII 246 GGTCC G^G[AT]CC
BbrPI 506 CACGTG CAC^GTG
BclI 74 TGATCA T^GATCA
BfaI 427 CTAG C^TAG
BlpI 680 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 680 GCTCAGC GC^T[ACGT]AGC
BsaAI 506 CACGTG [CT]AC^GT[GA]
BsaBI 31 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiYI 160 CCAAGGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 160 CCAAGGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 184 GAGAC GTCTC(1/5)
BsmI 97 GAATGC GAATGC(1/-1)
BspHI 180 TCATGA T^CATGA
BspMI 49 ACCTGC ACCTGC(4/8)
BssKI 250 CCTGG ^CC[ACGT]GG
BstAPI 149 GCAATGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 250 CCTGG CC^[AT]GG
CelII 680 GCTCAGC GC^T[ACGT]AGC
DraI 543 TTTAAA TTT^AAA
DraIII 19 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 622 GAATTC G^AATTC
EcoRII 250 CCTGG ^CC[AT]GG
EcoT14I 160 CCAAGG C^C[AT][AT]GG
FbaI 74 TGATCA T^GATCA
HaeIII 709 GGCC GG^CC
HinfI 280 GAATC G^A[ACGT]TC
MaeI 427 CTAG C^TAG
MaeII 507 ACGT A^CGT
MamI 31 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvaI 250 CCTGG CC^[AT]GG
MwoI 149 GCAATGATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 246 GGTCCC GG[ACGT]^[ACGT]CC
PflMI 160 CCAAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 506 CACGTG CAC^GTG
PmlI 506 CACGTG CAC^GTG
PstI 739 CTGCAG CTGCA^G
RcaI 180 TCATGA T^CATGA
RsaI 736 GTAC GT^AC
ScrFI 250 CCTGG CC^[ACGT]GG
SfcI 739 CTGCAG C^T[GA][CT]AG
StyI 160 CCAAGG C^C[AT][AT]GG
TfiI 280 GAATC G^A[AT]TC
TspRI 89 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 160 CCAAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 426 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 20 enzymes) #
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AflIII 505 ACACGT A^C[GA][CT]GT
507 ACGTGT
Asp700I 483 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
658 GAAAACTTTC
BsaJI 160 CCAAGG C^C[ACGT][ACGT]GG
249 CCCTGG
BsmFI 458 GGGAC GGGAC(10/14)
247 GTCCC
BsrDI 149 GCAATG GCAATG(2/0)
587 GCAATG
BstF5I 576 GGATG GGATG(2/0)
705 GGATG
Cfr13I 246 GGTCC G^G[ACGT]CC
709 GGCCC
DpnII 75 GATC ^GATC
610 GATC
FokI 576 GGATG GGATG(9/13)
705 GGATG
MboI 75 GATC ^GATC
610 GATC
MslI 397 CACAGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
627 CATCTACATG
NdeII 75 GATC ^GATC
610 GATC
NlaIII 181 CATG CATG^
633 CATG
Sau3AI 75 GATC ^GATC
610 GATC
Sau96I 246 GGTCC G^G[ACGT]CC
709 GGCCC
SmlI 271 CTTGAG C^T[CT][GA]AG
308 CTCAAG
TaqI 495 TCGA T^CGA
747 TCGA
Tsp45I 91 GTGAC ^GT[GC]AC
404 GTGAC
TthHB8I 495 TCGA T^CGA
747 TCGA
XmnI 483 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
658 GAAAACTTTC
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# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
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AcsI AluI ApoI DdeI HphI
MaeIII MboII MnlI MseI Tru9I
Tsp509I