Restriction map for
pF1KB7779
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AocI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BcgI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BseAI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspMI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DrdI
Eam1105I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI MaeI MamI MflI MluI
MroI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I StuI Tth111I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccII 405 CGCG CG^CG
Alw44I 1282 GTGCAC G^TGCAC
Aor51HI 234 AGCGCT AGC^GCT
ApaLI 1282 GTGCAC G^TGCAC
AspHI 1282 GTGCAC G[AT]GC[AT]^C
BanII 290 GAGCCC G[GA]GC[CT]^C
BbvCI 771 CCTCAGC CCTCAGC(-5/-2)
BcnI 35 CCGGG CC^[GC]GG
BsaI 274 GGTCTC GGTCTC(1/5)
BsaWI 1006 TCCGGT [AT]^CCGG[AT]
BsiEI 932 CGGCCG CG[GA][CT]^CG
BsiHKAI 1282 GTGCAC G[AT]GC[AT]^C
Bsp1407I 1190 TGTACA T^GTACA
BsrGI 1190 TGTACA T^GTACA
BstAPI 391 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 405 CGCG CG^CG
DraI 1206 TTTAAA TTT^AAA
DraIII 55 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 404 CCGCGG C^C[GA][CT]GG
EagI 932 CGGCCG C^GGCCG
EarI 238 CTCTTC CTCTTC(1/4)
EclXI 932 CGGCCG C^GGCCG
Eco47III 234 AGCGCT AGC^GCT
Eco52I 932 CGGCCG C^GGCCG
FauI 90 CCCGC CCCGC(4/6)
HaeII 234 AGCGCT [GA]GCGC^[CT]
Ksp632I 238 CTCTTC CTCTTC(1/4)
KspI 404 CCGCGG CCGC^GG
MaeII 573 ACGT A^CGT
MfeI 849 CAATTG C^AATTG
MunI 849 CAATTG C^AATTG
MvnI 405 CGCG CG^CG
NciI 35 CCGGG CC^[GC]GG
SacII 404 CCGCGG CCGC^GG
SapI 237 GCTCTTC GCTCTTC(1/4)
SspBI 1190 TGTACA T^GTACA
SspI 755 AATATT AAT^ATT
SwaI 1205 ATTTAAAT ATTT^AAAT
TaqI 621 TCGA T^CGA
TthHB8I 621 TCGA T^CGA
XcmI 800 CCAAAGAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AfaI 876 GTAC GT^AC
1191 GTAC
AlwI 313 GATCC GGATC(4/5)
382 GGATC
BalI 51 TGGCCA TGG^CCA
812 TGGCCA
BmyI 290 GAGCCC G[AGT]GC[ACT]^C
1282 GTGCAC
BpmI 416 CTGGAG CTGGAG(16/14)
499 CTGGAG
BsmAI 327 GAGAC GTCTC(1/5)
275 GTCTC
Bsp1286I 290 GAGCCC G[AGT]GC[ACT]^C
1282 GTGCAC
BspHI 158 TCATGA T^CATGA
1052 TCATGA
BspLU11I 537 ACATGT A^CATGT
1046 ACATGT
CfoI 190 GCGC GCG^C
235 GCGC
EcoNI 367 CCTGAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1261 CCTACAGAAGG
EcoT14I 1086 CCAAGG C^C[AT][AT]GG
1173 CCTTGG
HapII 35 CCGG C^CGG
1007 CCGG
HgaI 1231 GACGC GACGC(5/10)
245 GCGTC
HhaI 190 GCGC GCG^C
235 GCGC
HinP1I 190 GCGC G^CGC
235 GCGC
HpaII 35 CCGG C^CGG
1007 CCGG
HphI 39 GGTGA GGTGA(8/7)
167 TCACC
MluNI 51 TGGCCA TGG^CCA
812 TGGCCA
MscI 51 TGGCCA TGG^CCA
812 TGGCCA
MspI 35 CCGG C^CGG
1007 CCGG
PflMI 174 CCAGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1033 CCAAATTCTGG
PleI 27 GACTC GAGTC(4/5)
1065 GAGTC
PstI 22 CTGCAG CTGCA^G
398 CTGCAG
RcaI 158 TCATGA T^CATGA
1052 TCATGA
RsaI 876 GTAC GT^AC
1191 GTAC
StyI 1086 CCAAGG C^C[AT][AT]GG
1173 CCTTGG
TfiI 869 GAATC G^A[AT]TC
1078 GATTC
Van91I 174 CCAGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1033 CCAAATTCTGG
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AcsI AflIII AluI AlwNI
ApoI AvaII BbvI BciVI BsaJI
BseRI BsgI BsiYI BslI BsmFI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HinfI ItaI MaeIII MboI MboII
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PpuMI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI Tru9I TseI Tsp45I
Tsp509I TspRI