Restriction map for
pF1KB7723
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BcgI BciVI BclI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BspDI BspEI BsrBI
BsrDI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
ClaI CpoI DraI DraIII EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MseI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tru9I Tsp509I Tth111I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AfaI 1179 GTAC GT^AC
AflIII 1061 ACATGT A^C[GA][CT]GT
BfaI 815 CTAG C^TAG
BglI 41 GCCGGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 404 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 404 GCTCAGC GC^T[ACGT]AGC
BsmI 302 GCATTC GAATGC(1/-1)
Bsp1407I 1178 TGTACA T^GTACA
BspHI 569 TCATGA T^CATGA
BspLU11I 1061 ACATGT A^CATGT
BsrFI 327 GCCGGT [GA]^CCGG[CT]
BsrGI 1178 TGTACA T^GTACA
CelII 404 GCTCAGC GC^T[ACGT]AGC
Cfr10I 327 GCCGGT [GA]^CCGG[CT]
DrdI 957 GACCGCCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1233 CTCTTC CTCTTC(1/4)
EcoNI 201 CCTGGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 827 CCAAGG C^C[AT][AT]GG
HgaI 266 GCGTC GACGC(5/10)
Ksp632I 1233 CTCTTC CTCTTC(1/4)
MaeI 815 CTAG C^TAG
MboII 1234 TCTTC GAAGA(8/7)
PflMI 195 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 561 GACCCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 569 TCATGA T^CATGA
RsaI 1179 GTAC GT^AC
SacI 309 GAGCTC GAGCT^C
SfiI 40 GGCCGGGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 132 CCCGGG CCC^GGG
SspBI 1178 TGTACA T^GTACA
StyI 827 CCAAGG C^C[AT][AT]GG
Van91I 195 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 132 CCCGGG C^CCGGG
XmaI 132 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
ApaI 78 GGGCCC GGGCC^C
385 GGGCCC
AspHI 309 GAGCTC G[AT]GC[AT]^C
684 GAGCAC
BbvCI 297 CCTCAGC CCTCAGC(-5/-2)
579 GCTGAGG
BsaI 104 GAGACC GGTCTC(1/5)
870 GGTCTC
BsiHKAI 309 GAGCTC G[AT]GC[AT]^C
684 GAGCAC
BspMI 575 ACCTGC ACCTGC(4/8)
688 ACCTGC
BstAPI 412 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
421 GCAGCCGCTGC
BstXI 599 CCAGGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
614 CCAAAGAGATGG
BstYI 500 AGATCC [GA]^GATC[CT]
1051 GGATCT
DsaI 605 CCGTGG C^C[GA][CT]GG
1186 CCACGG
FauI 35 GCGGG CCCGC(4/6)
122 GCGGG
HaeII 658 AGCGCC [GA]GCGC^[CT]
740 AGCGCC
MflI 500 AGATCC [GA]^GATC[CT]
1051 GGATCT
MslI 663 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
855 CATCACCGTG
PpuMI 128 GGGTCCC [GA]G^G[AT]CC[CT]
770 AGGTCCT
TspRI 765 CACTG CA[GC]TG[ACGT][ACGT]^
1238 CAGTG
XhoII 500 AGATCC [GA]^GATC[CT]
1051 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PstI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI TseI Tsp45I TthHB8I