Restriction map for
pF1KB7675
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BamHI BbeI
BbrPI BbsI BbvCI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsgI BsiWI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DdeI
DraI DraII DraIII DrdI Eam1105I
EarI Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MaeII MamI
MfeI MluI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp509I
Tth111I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 260 TCCGGA T^CCGGA
AcyI 444 GACGCC G[GA]^CG[CT]C
Aor51HI 456 AGCGCT AGC^GCT
AspHI 360 GAGCTC G[AT]GC[AT]^C
AviII 380 TGCGCA TGC^GCA
BalI 557 TGGCCA TGG^CCA
BcgI 383 GCAACAAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 745 CTGGAG CTGGAG(16/14)
BsaHI 444 GACGCC G[GA]^CG[CT]C
BsaWI 260 TCCGGA [AT]^CCGG[AT]
BseAI 260 TCCGGA T^CCGGA
BsiHKAI 360 GAGCTC G[AT]GC[AT]^C
BsmBI 184 GAGACG CGTCTC(1/5)
BsmFI 308 GGGAC GGGAC(10/14)
BspEI 260 TCCGGA T^CCGGA
BsrBI 612 CCGCTC CCGCTC(-3/-3)
DsaI 245 CCGTGG C^C[GA][CT]GG
EagI 506 CGGCCG C^GGCCG
EclXI 506 CGGCCG C^GGCCG
Eco47III 456 AGCGCT AGC^GCT
Eco52I 506 CGGCCG C^GGCCG
EcoNI 744 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 380 TGCGCA TGC^GCA
HaeII 456 AGCGCT [GA]GCGC^[CT]
Hin1I 444 GACGCC G[GA]^CG[CT]C
HinfI 421 GACTC G^A[ACGT]TC
MboII 290 TCTTC GAAGA(8/7)
MluNI 557 TGGCCA TGG^CCA
MroI 260 TCCGGA T^CCGGA
MscI 557 TGGCCA TGG^CCA
NotI 505 GCGGCCGC GC^GGCCGC
PleI 421 GACTC GAGTC(4/5)
SacI 360 GAGCTC GAGCT^C
SfcI 864 CTACAG C^T[GA][CT]AG
SphI 714 GCATGC GCATG^C
TaqI 193 TCGA T^CGA
TspRI 867 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 193 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 165 GATCC GGATC(4/5)
303 GATCC
AvaI 227 CCCGGG C^[CT]CG[GA]G
529 CCCGGG
AvaII 263 GGACC G^G[AT]CC
309 GGACC
BsiEI 112 CGACCG CG[GA][CT]^CG
506 CGGCCG
BsmI 974 GAATGC GAATGC(1/-1)
374 GCATTC
BsoBI 227 CCCGGG C^[CT]CG[GA]G
529 CCCGGG
BsrI 93 ACTGG ACTGG(1/-1)
102 ACTGG
BstYI 164 AGATCC [GA]^GATC[CT]
302 AGATCC
EaeI 506 CGGCCG [CT]^GGCC[GA]
557 TGGCCA
EcoT14I 512 CCAAGG C^C[AT][AT]GG
659 CCAAGG
FauI 700 CCCGC CCCGC(4/6)
711 CCCGC
HgaI 444 GACGC GACGC(5/10)
155 GCGTC
MflI 164 AGATCC [GA]^GATC[CT]
302 AGATCC
MslI 243 CACCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
937 CACAACTGTG
NspI 714 GCATGC [GA]CATG^[CT]
909 ACATGC
PvuII 28 CAGCTG CAG^CTG
788 CAGCTG
SmaI 227 CCCGGG CCC^GGG
529 CCCGGG
StyI 512 CCAAGG C^C[AT][AT]GG
659 CCAAGG
XhoII 164 AGATCC [GA]^GATC[CT]
302 AGATCC
XmaCI 227 CCCGGG C^CCGGG
529 CCCGGG
XmaI 227 CCCGGG C^CCGGG
529 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AfaI AluI BanI
BanII BbvI BcnI BmyI BsaI
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DpnII EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeIII
MboI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI TseI Tsp45I