Restriction map for
pF1KB6832
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BseAI BseRI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DpnII
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MaeII MamI
MboI MflI MluI MluNI MroI
MscI MslI MspA1I MvnI NaeI
NarI NcoI NdeII NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 240 GAATTT [GA]^AATT[CT]
AfaI 130 GTAC GT^AC
ApoI 240 GAATTT [GA]^AATT[CT]
AspHI 14 GAGCTC G[AT]GC[AT]^C
BanII 14 GAGCTC G[GA]GC[CT]^C
BbvI 256 GCTGC GCAGC(8/12)
BcnI 465 CCGGG CC^[GC]GG
BmyI 14 GAGCTC G[AGT]GC[ACT]^C
BsaWI 290 ACCGGA [AT]^CCGG[AT]
BsgI 317 GTGCAG GTGCAG(16/14)
BsiHKAI 14 GAGCTC G[AT]GC[AT]^C
BsiYI 141 CCGTGATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 141 CCGTGATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 52 GAGAC GTCTC(1/5)
BsmBI 52 GAGACG CGTCTC(1/5)
Bsp1286I 14 GAGCTC G[AGT]GC[ACT]^C
BspMI 319 GCAGGT ACCTGC(4/8)
BsrI 127 CCAGT ACTGG(1/-1)
BstEII 96 GGTCACC G^GT[ACGT]ACC
BstPI 96 GGTCACC G^GT[ACGT]ACC
EcoO65I 96 GGTCACC G^GT[ACGT]ACC
HphI 98 TCACC GGTGA(8/7)
MboII 175 GAAGA GAAGA(8/7)
MfeI 500 CAATTG C^AATTG
MunI 500 CAATTG C^AATTG
NciI 465 CCGGG CC^[GC]GG
NdeI 197 CATATG CA^TATG
PleI 250 GAGTC GAGTC(4/5)
PpuMI 321 AGGTCCT [GA]G^G[AT]CC[CT]
RsaI 130 GTAC GT^AC
SacI 14 GAGCTC GAGCT^C
SfcI 65 CTGTAG C^T[GA][CT]AG
SmlI 536 CTCAAG C^T[CT][GA]AG
StuI 108 AGGCCT AGG^CCT
TaqI 485 TCGA T^CGA
TseI 256 GCTGC G^C[AT]GC
Tsp45I 97 GTCAC ^GT[GC]AC
TspRI 133 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 485 TCGA T^CGA
XcmI 8 CCAAAAGAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 308 CCGC CCGC(-3/-1)
532 GCGG
BsaJI 465 CCGGGG C^C[ACGT][ACGT]GG
472 CCTGGG
BsmFI 192 GGGAC GGGAC(10/14)
475 GGGAC
BspHI 74 TCATGA T^CATGA
342 TCATGA
BstF5I 24 CATCC GGATG(2/0)
60 CATCC
CfoI 233 GCGC GCG^C
493 GCGC
DdeI 248 CTGAG C^T[ACGT]AG
425 CTGAG
FokI 24 CATCC GGATG(9/13)
60 CATCC
HapII 291 CCGG C^CGG
465 CCGG
HhaI 233 GCGC GCG^C
493 GCGC
HinP1I 233 GCGC G^CGC
493 GCGC
HpaII 291 CCGG C^CGG
465 CCGG
MaeIII 97 GTCAC ^GT[ACGT]AC
367 GTTAC
MseI 92 TTAA T^TAA
560 TTAA
MspI 291 CCGG C^CGG
465 CCGG
NlaIII 75 CATG CATG^
343 CATG
RcaI 74 TCATGA T^CATGA
342 TCATGA
SfaNI 163 GATGC GCATC(5/9)
178 GATGC
TfiI 429 GAATC G^A[AT]TC
453 GATTC
Tru9I 92 TTAA T^TAA
560 TTAA
Tsp509I 241 AATT ^AATT
501 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AluI AvaII BssKI BstNI Cac8I
Cfr13I DraII EcoO109I EcoRII Fnu4HI
HaeIII HinfI ItaI MnlI MvaI
MwoI NlaIV Sau96I ScrFI