Restriction map for
pF1KB6528
##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbsI BbvCI BfrI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaHI
BseRI BsgI BsiWI BsmBI BsmI
Bsp1407I BspDI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HaeII HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MfeI MluI MluNI MscI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 242 TCCGGA T^CCGGA
AlwNI 337 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 349 CCCGAG C^[CT]CG[GA]G
AvaII 1158 GGACC G^G[AT]CC
BanII 85 GGGCTC G[GA]GC[CT]^C
BbrPI 514 CACGTG CAC^GTG
BcgI 947 GCATCCGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 582 GTATCC GTATCC(6/5)
BclI 1007 TGATCA T^GATCA
BcnI 495 CCCGG CC^[GC]GG
BglI 16 GCCGCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 514 CACGTG [CT]AC^GT[GA]
BsaBI 1008 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 902 GAGACC GGTCTC(1/5)
BsaWI 242 TCCGGA [AT]^CCGG[AT]
BseAI 242 TCCGGA T^CCGGA
BsiEI 1046 CGACCG CG[GA][CT]^CG
BsoBI 349 CCCGAG C^[CT]CG[GA]G
BspEI 242 TCCGGA T^CCGGA
BsrI 773 ACTGG ACTGG(1/-1)
DdeI 278 CTCAG C^T[ACGT]AG
DraI 534 TTTAAA TTT^AAA
DraII 338 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 369 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 737 TGGCCG [CT]^GGCC[GA]
EarI 295 CTCTTC CTCTTC(1/4)
EcoO109I 338 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 197 CCAAGG C^C[AT][AT]GG
FauI 4 GCGGG CCCGC(4/6)
FbaI 1007 TGATCA T^GATCA
HincII 769 GTCAAC GT[CT]^[GA]AC
HindII 769 GTCAAC GT[CT]^[GA]AC
Ksp632I 295 CTCTTC CTCTTC(1/4)
MamI 1008 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 242 TCCGGA T^CCGGA
MspA1I 390 CAGCTG C[AC]G^C[GT]G
NciI 495 CCCGG CC^[GC]GG
PflMI 729 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 514 CACGTG CAC^GTG
PmlI 514 CACGTG CAC^GTG
PshAI 195 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 542 CTGCAG CTGCA^G
PvuII 390 CAGCTG CAG^CTG
ScaI 893 AGTACT AGT^ACT
SmlI 640 CTCAAG C^T[CT][GA]AG
StuI 938 AGGCCT AGG^CCT
StyI 197 CCAAGG C^C[AT][AT]GG
Van91I 729 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 589 AAATTT [GA]^AATT[CT]
985 AAATTT
AflIII 472 ACGTGT A^C[GA][CT]GT
513 ACACGT
ApoI 589 AAATTT [GA]^AATT[CT]
985 AAATTT
AspHI 61 GTGCTC G[AT]GC[AT]^C
853 GAGCAC
BanI 412 GGCACC G^G[CT][GA]CC
491 GGTGCC
BfaI 1036 CTAG C^TAG
1148 CTAG
BsiHKAI 61 GTGCTC G[AT]GC[AT]^C
853 GAGCAC
BsmAI 902 GAGAC GTCTC(1/5)
430 GTCTC
BsmFI 776 GGGAC GGGAC(10/14)
845 GGGAC
BspHI 89 TCATGA T^CATGA
302 TCATGA
BspMI 525 ACCTGC ACCTGC(4/8)
440 GCAGGT
BsrFI 400 GCCGGC [GA]^CCGG[CT]
409 GCCGGC
BstYI 245 GGATCT [GA]^GATC[CT]
669 AGATCC
Cfr10I 400 GCCGGC [GA]^CCGG[CT]
409 GCCGGC
Cfr13I 339 GGGCC G^G[ACGT]CC
1158 GGACC
DrdI 172 GACTCCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1060 GACATCATAGTC
DsaI 176 CCGTGG C^C[GA][CT]GG
740 CCGTGG
HphI 320 TCACC GGTGA(8/7)
626 TCACC
MaeI 1036 CTAG C^TAG
1148 CTAG
MflI 245 GGATCT [GA]^GATC[CT]
669 AGATCC
NaeI 400 GCCGGC GCC^GGC
409 GCCGGC
NgoMIV 400 GCCGGC G^CCGGC
409 GCCGGC
NlaIV 412 GGCACC GG[ACGT]^[ACGT]CC
491 GGTGCC
RcaI 89 TCATGA T^CATGA
302 TCATGA
Sau96I 339 GGGCC G^G[ACGT]CC
1158 GGACC
SfcI 542 CTGCAG C^T[GA][CT]AG
897 CTACAG
TfiI 239 GATTC G^A[AT]TC
354 GATTC
Tsp45I 217 GTGAC ^GT[GC]AC
1058 GTGAC
TspRI 369 CACTG CA[GC]TG[ACGT][ACGT]^
1031 CACTG
XhoII 245 GGATCT [GA]^GATC[CT]
669 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
BbvI BmyI BsaJI BsiYI BslI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I CfoI DpnII EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MvnI MwoI NdeII
NlaIII PleI RsaI Sau3AI ScrFI
SfaNI TaqI Tru9I TseI Tsp509I
TthHB8I