Restriction map for
pF1KB6410
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BbeI BbrPI
BbsI BbvCI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1407I BspEI BspHI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I CelII CpoI DraI
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoRI EcoT22I FbaI
FseI FspI HgaI HincII HindII
HindIII HpaI KasI KpnI Ksp632I
MamI MfeI MluI MroI MunI
NarI NdeI NheI NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SapI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AatII 601 GACGTC GACGT^C
AccII 83 CGCG CG^CG
AcyI 601 GACGTC G[GA]^CG[CT]C
AlwNI 185 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 676 CCTGAGG CC^T[ACGT]AGG
ApaI 234 GGGCCC GGGCC^C
AvaI 494 CTCGAG C^[CT]CG[GA]G
AvaII 935 GGACC G^G[AT]CC
BalI 656 TGGCCA TGG^CCA
BamHI 617 GGATCC G^GATCC
BanI 247 GGTGCC G^G[CT][GA]CC
BcgI 213 CGACTGCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 529 GGATAC GTATCC(6/5)
BcnI 845 CCCGG CC^[GC]GG
BfaI 160 CTAG C^TAG
BsaHI 601 GACGTC G[GA]^CG[CT]C
BseRI 782 GAGGAG GAGGAG(10/8)
BsmAI 604 GTCTC GTCTC(1/5)
BsmBI 603 CGTCTC CGTCTC(1/5)
BsmFI 1001 GGGAC GGGAC(10/14)
BsoBI 494 CTCGAG C^[CT]CG[GA]G
BspDI 457 ATCGAT AT^CGAT
BsrFI 3 GCCGGC [GA]^CCGG[CT]
BstEII 990 GGTGACC G^GT[ACGT]ACC
BstPI 990 GGTGACC G^GT[ACGT]ACC
BstUI 83 CGCG CG^CG
BstXI 831 CCAGGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 676 CCTGAGG CC^T[ACGT]AGG
CfoI 29 GCGC GCG^C
Cfr10I 3 GCCGGC [GA]^CCGG[CT]
ClaI 457 ATCGAT AT^CGAT
EaeI 656 TGGCCA [CT]^GGCC[GA]
Eco81I 676 CCTGAGG CC^T[ACGT]AGG
EcoO65I 990 GGTGACC G^GT[ACGT]ACC
EcoRV 208 GATATC GAT^ATC
HaeII 28 AGCGCC [GA]GCGC^[CT]
HhaI 29 GCGC GCG^C
Hin1I 601 GACGTC G[GA]^CG[CT]C
HinP1I 29 GCGC G^CGC
KspI 82 CCGCGG CCGC^GG
MaeI 160 CTAG C^TAG
MboII 504 GAAGA GAAGA(8/7)
MluNI 656 TGGCCA TGG^CCA
MscI 656 TGGCCA TGG^CCA
MseI 196 TTAA T^TAA
MslI 339 CATTGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 83 CGCG CG^CG
NaeI 3 GCCGGC GCC^GGC
NciI 845 CCCGG CC^[GC]GG
NcoI 743 CCATGG C^CATGG
NgoMIV 3 GCCGGC G^CCGGC
PaeR7I 494 CTCGAG C^TCGAG
PleI 187 GAGTC GAGTC(4/5)
PpuMI 934 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 292 CAGCTG CAG^CTG
SacII 82 CCGCGG CCGC^GG
SbfI 696 CCTGCAGG CCTGCA^GG
SmlI 494 CTCGAG C^T[CT][GA]AG
Sse8387I 696 CCTGCAGG CCTGCA^GG
Tru9I 196 TTAA T^TAA
Tsp509I 475 AATT ^AATT
XhoI 494 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 281 GTAC GT^AC
671 GTAC
BanII 234 GGGCCC G[GA]GC[CT]^C
842 GAGCCC
BpmI 362 CTGGAG CTGGAG(16/14)
996 CTGGAG
BspMI 164 ACCTGC ACCTGC(4/8)
408 GCAGGT
BsrDI 219 CATTGC GCAATG(2/0)
715 GCAATG
BsrI 255 ACTGG ACTGG(1/-1)
361 ACTGG
BstYI 617 GGATCC [GA]^GATC[CT]
681 GGATCT
DraIII 749 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
941 CACTCAGTG
DsaI 82 CCGCGG C^C[GA][CT]GG
743 CCATGG
EcoNI 106 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
165 CCTGCTGCAGG
EcoT14I 230 CCAAGG C^C[AT][AT]GG
743 CCATGG
FauI 81 CCCGC CCCGC(4/6)
57 GCGGG
HapII 4 CCGG C^CGG
846 CCGG
HpaII 4 CCGG C^CGG
846 CCGG
MflI 617 GGATCC [GA]^GATC[CT]
681 GGATCT
MspA1I 82 CCGCGG C[AC]G^C[GT]G
292 CAGCTG
MspI 4 CCGG C^CGG
846 CCGG
NlaIII 243 CATG CATG^
744 CATG
PshAI 90 GACTTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
517 GACCCAAGTC
PstI 169 CTGCAG CTGCA^G
697 CTGCAG
RsaI 281 GTAC GT^AC
671 GTAC
SfaNI 651 GATGC GCATC(5/9)
141 GCATC
StyI 230 CCAAGG C^C[AT][AT]GG
743 CCATGG
Tsp45I 97 GTCAC ^GT[GC]AC
991 GTGAC
XhoII 617 GGATCC [GA]^GATC[CT]
681 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI AlwI BbvI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeII MaeIII MboI MnlI MvaI
MwoI NdeII NlaIV Sau3AI Sau96I
ScrFI SfcI TaqI TfiI TseI
TspRI TthHB8I