Restriction map for
pF1KB7134
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BanII BbeI BbrPI
BbvCI BcgI BcnI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmAI
BsmBI BsmFI BsmI BspDI BspEI
BspHI BspLU11I BsrFI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HaeII HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
KasI KpnI Ksp632I MaeI MfeI
MluI MroI MseI MspI MunI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspI SwaI TaqI Tru9I Tsp509I
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccII 448 CGCG CG^CG
AspHI 400 GTGCTC G[AT]GC[AT]^C
AvaI 263 CCCGAG C^[CT]CG[GA]G
AvaII 444 GGTCC G^G[AT]CC
BamHI 451 GGATCC G^GATCC
BanI 874 GGTGCC G^G[CT][GA]CC
BbsI 133 GTCTTC GAAGAC(2/6)
BciVI 44 GTATCC GTATCC(6/5)
BclI 482 TGATCA T^GATCA
BmyI 400 GTGCTC G[AGT]GC[ACT]^C
BpuAI 133 GTCTTC GAAGAC(2/6)
BsaBI 426 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 160 CTGCAC GTGCAG(16/14)
BsiHKAI 400 GTGCTC G[AT]GC[AT]^C
BsoBI 263 CCCGAG C^[CT]CG[GA]G
Bsp1286I 400 GTGCTC G[AGT]GC[ACT]^C
Bsp1407I 777 TGTACA T^GTACA
BspMI 836 ACCTGC ACCTGC(4/8)
BsrBI 694 GAGCGG CCGCTC(-3/-3)
BsrDI 109 GCAATG GCAATG(2/0)
BsrGI 777 TGTACA T^GTACA
BstUI 448 CGCG CG^CG
CfoI 387 GCGC GCG^C
FauI 696 GCGGG CCCGC(4/6)
FbaI 482 TGATCA T^GATCA
HhaI 387 GCGC GCG^C
HinP1I 387 GCGC G^CGC
HphI 826 GGTGA GGTGA(8/7)
KspI 447 CCGCGG CCGC^GG
MaeII 626 ACGT A^CGT
MamI 426 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 134 TCTTC GAAGA(8/7)
MvnI 448 CGCG CG^CG
NcoI 347 CCATGG C^CATGG
PflMI 757 CCACTTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 395 CTGCAG CTGCA^G
PvuII 924 CAGCTG CAG^CTG
SacII 447 CCGCGG CCGC^GG
SfcI 395 CTGCAG C^T[GA][CT]AG
SphI 188 GCATGC GCATG^C
SspBI 777 TGTACA T^GTACA
Van91I 757 CCACTTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AluI 274 AGCT AG^CT
925 AGCT
BalI 344 TGGCCA TGG^CCA
368 TGGCCA
BstYI 236 AGATCC [GA]^GATC[CT]
451 GGATCC
Cfr13I 444 GGTCC G^G[ACGT]CC
698 GGGCC
DsaI 347 CCATGG C^C[GA][CT]GG
447 CCGCGG
EaeI 344 TGGCCA [CT]^GGCC[GA]
368 TGGCCA
EcoT14I 347 CCATGG C^C[AT][AT]GG
701 CCAAGG
HinfI 259 GATTC G^A[ACGT]TC
328 GAATC
MflI 236 AGATCC [GA]^GATC[CT]
451 GGATCC
MluNI 344 TGGCCA TGG^CCA
368 TGGCCA
MscI 344 TGGCCA TGG^CCA
368 TGGCCA
MspA1I 447 CCGCGG C[AC]G^C[GT]G
924 CAGCTG
MwoI 313 GCCCTCTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
480 GCTGATCAAGC
NspI 21 ACATGC [GA]CATG^[CT]
188 GCATGC
Sau96I 444 GGTCC G^G[ACGT]CC
698 GGGCC
SfaNI 125 GCATC GCATC(5/9)
431 GCATC
StuI 281 AGGCCT AGG^CCT
704 AGGCCT
StyI 347 CCATGG C^C[AT][AT]GG
701 CCAAGG
TfiI 259 GATTC G^A[AT]TC
328 GAATC
XhoII 236 AGATCC [GA]^GATC[CT]
451 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AfaI AlwI BbvI BsaJI
BsiYI BslI BssKI BstF5I BstNI
BstXI Cac8I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII ItaI MaeIII
MboI MnlI MslI MvaI NdeII
NlaIII NlaIV RsaI Sau3AI ScrFI
TseI Tsp45I TspRI XcmI