##################################
# NO CUT ENZYMES ( 168 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvrII BalI BamHI BanII BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaI BsaWI
BseAI BsiHKAI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI DdeI DpnII DraI DraII
DrdI DsaI Eam1105I EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI HincII
HindII HindIII HinfI HpaI HphI
KpnI Ksp632I KspI MaeII MaeIII
MboI MfeI MflI MluI MluNI
MroI MscI MslI MunI NcoI
NdeI NdeII NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI SacI
SacII SalI SapI Sau3AI SbfI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI TfiI Tsp45I Tsp509I Tth111I
TthHB8I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AfaI 300 GTAC GT^AC
AlwNI 518 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 402 AGCGCT AGC^GCT
BfaI 289 CTAG C^TAG
BmyI 192 GGGCAC G[AGT]GC[ACT]^C
BsaBI 171 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 418 GTGCAG GTGCAG(16/14)
BsiEI 498 CGGCCG CG[GA][CT]^CG
BsmFI 368 GGGAC GGGAC(10/14)
Bsp1286I 192 GGGCAC G[AGT]GC[ACT]^C
BssHII 477 GCGCGC G^CGCGC
BstF5I 45 CATCC GGATG(2/0)
CpoI 502 CGGTCCG CG^G[AT]CCG
EaeI 498 CGGCCG [CT]^GGCC[GA]
EagI 498 CGGCCG C^GGCCG
EclXI 498 CGGCCG C^GGCCG
Eco47III 402 AGCGCT AGC^GCT
Eco52I 498 CGGCCG C^GGCCG
FokI 45 CATCC GGATG(9/13)
HgaI 526 GCGTC GACGC(5/10)
MaeI 289 CTAG C^TAG
MamI 171 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 80 TTAA T^TAA
MspA1I 208 CCGCTG C[AC]G^C[GT]G
NaeI 132 GCCGGC GCC^GGC
NgoMIV 132 GCCGGC G^CCGGC
NlaIII 180 CATG CATG^
PstI 406 CTGCAG CTGCA^G
RsaI 300 GTAC GT^AC
RsrII 502 CGGTCCG CG^G[AT]CCG
ScaI 299 AGTACT AGT^ACT
SfcI 406 CTGCAG C^T[GA][CT]AG
Tru9I 80 TTAA T^TAA
XcmI 263 CCATCTTCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AvaII 164 GGACC G^G[AT]CC
503 GGTCC
AviII 173 TGCGCA TGC^GCA
455 TGCGCA
BcnI 189 CCGGG CC^[GC]GG
496 CCCGG
BpmI 295 CTGGAG CTGGAG(16/14)
445 CTGGAG
BsaJI 189 CCGGGG C^C[ACGT][ACGT]GG
443 CCCTGG
BspMI 51 GCAGGT ACCTGC(4/8)
414 GCAGGT
BsrFI 132 GCCGGC [GA]^CCGG[CT]
500 GCCGGT
BsrI 149 ACTGG ACTGG(1/-1)
302 ACTGG
BstAPI 468 GCAAGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
517 GCAGTGCCTGC
Cfr10I 132 GCCGGC [GA]^CCGG[CT]
500 GCCGGT
DraIII 148 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
514 CACGCAGTG
FspI 173 TGCGCA TGC^GCA
455 TGCGCA
MboII 107 TCTTC GAAGA(8/7)
266 TCTTC
NciI 189 CCGGG CC^[GC]GG
496 CCCGG
TspRI 148 CACTG CA[GC]TG[ACGT][ACGT]^
518 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AccII AciI AcyI AluI BanI
BbeI BbvI BsaHI BseRI BsiYI
BslI BssKI BstNI BstUI Cac8I
CfoI Cfr13I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII ItaI KasI MnlI
MspI MvaI MvnI MwoI NarI
NlaIV Sau96I ScrFI SfaNI SfoI
TseI