##################################
# NO CUT ENZYMES (  201 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      Bst1107I    BstAPI      BstBI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DdeI        DpnII       
DraI        DraII       DraIII      DrdI        DsaI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MamI        MboI        MfeI        
MflI        MluI        MroI        MseI        MslI        
MspA1I      MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NlaIII      NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        Sau3AI      SbfI        
ScaI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        Tru9I       
TspRI       Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AcsI          213  GAATTC               [GA]^AATT[CT]
ApoI          213  GAATTC               [GA]^AATT[CT]
BalI            2  TGGCCA               TGG^CCA
BcnI           48  CCCGG                CC^[GC]GG
BpmI           63  CTCCAG               CTGGAG(16/14)
BsrI          195  ACTGG                ACTGG(1/-1)
BssSI         184  CACGAG               CACGAG(-5/-1)
BstEII        198  GGTGACC              G^GT[ACGT]ACC
BstF5I         72  GGATG                GGATG(2/0)
BstPI         198  GGTGACC              G^GT[ACGT]ACC
Cac8I         179  GCGGGC               GC[ACGT]^[ACGT]GC
EaeI            2  TGGCCA               [CT]^GGCC[GA]
EcoO65I       198  GGTGACC              G^GT[ACGT]ACC
EcoRI         213  GAATTC               G^AATTC
FauI          179  GCGGG                CCCGC(4/6)
FokI           72  GGATG                GGATG(9/13)
HaeII          25  AGCGCC               [GA]GCGC^[CT]
HphI          198  GGTGA                GGTGA(8/7)
MaeIII        199  GTGAC                ^GT[ACGT]AC
MboII         225  GAAGA                GAAGA(8/7)
MluNI           2  TGGCCA               TGG^CCA
MscI            2  TGGCCA               TGG^CCA
MwoI           19  GCAACCAGCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI           48  CCCGG                CC^[GC]GG
PleI           61  GACTC                GAGTC(4/5)
PstI          208  CTGCAG               CTGCA^G
TfiI           76  GAATC                G^A[AT]TC
Tsp45I        199  GTGAC                ^GT[GC]AC
Tsp509I       214  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AciI          165  CCGC                 CCGC(-3/-1)
              179  GCGG                
AluI          147  AGCT                 AG^CT
              206  AGCT                
BmyI          181  GGGCAC               G[AGT]GC[ACT]^C
              236  GGGCAC              
BsiYI          42  CCCAGGCCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              169  CCCAGCCCTGG         
BslI           42  CCCAGGCCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              169  CCCAGCCCTGG         
Bsp1286I      181  GGGCAC               G[AGT]GC[ACT]^C
              236  GGGCAC              
CfoI           26  GCGC                 GCG^C
              243  GCGC                
Cfr13I         46  GGCCC                G^G[ACGT]CC
               96  GGCCC               
HapII          49  CCGG                 C^CGG
              123  CCGG                
HhaI           26  GCGC                 GCG^C
              243  GCGC                
HinP1I         26  GCGC                 G^CGC
              243  GCGC                
HinfI          61  GACTC                G^A[ACGT]TC
               76  GAATC               
HpaII          49  CCGG                 C^CGG
              123  CCGG                
MspI           49  CCGG                 C^CGG
              123  CCGG                
Sau96I         46  GGCCC                G^G[ACGT]CC
               96  GGCCC               
SfcI           87  CTATAG               C^T[GA][CT]AG
              208  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

BbvI        BsaJI       BssKI       BstNI       EcoRII      
Fnu4HI      HaeIII      ItaI        MnlI        MvaI        
ScrFI       TseI