Restriction map for
pF1KE0167
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AgeI AhdI AlwNI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I AspEI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaI
BsgI BsiEI BsmAI BsmBI BsmFI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI ClaI DdeI DraI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HhaI HinP1I
HincII HindII HindIII HpaI HphI
KasI Ksp632I KspI MaeI MfeI
MflI MluI MluNI MscI MseI
MslI MunI NarI NdeI NheI
NotI NruI NsiI NspI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp509I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 119 GGTACC G^GTACC
AccIII 74 TCCGGA T^CCGGA
AcyI 241 GGCGTC G[GA]^CG[CT]C
AflIII 281 ACGTGT A^C[GA][CT]GT
Alw44I 403 GTGCAC G^TGCAC
ApaLI 403 GTGCAC G^TGCAC
Asp718I 119 GGTACC G^GTACC
AspHI 403 GTGCAC G[AT]GC[AT]^C
AspI 91 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 119 GGTACC G^G[CT][GA]CC
BanII 157 GAGCCC G[GA]GC[CT]^C
BbsI 244 GTCTTC GAAGAC(2/6)
BpuAI 244 GTCTTC GAAGAC(2/6)
BsaAI 280 TACGTG [CT]AC^GT[GA]
BsaBI 481 GATAGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 241 GGCGTC G[GA]^CG[CT]C
BsaWI 74 TCCGGA [AT]^CCGG[AT]
BseAI 74 TCCGGA T^CCGGA
BsiHKAI 403 GTGCAC G[AT]GC[AT]^C
BsiWI 278 CGTACG C^GTACG
BspEI 74 TCCGGA T^CCGGA
BsrI 387 CCAGT ACTGG(1/-1)
BstBI 247 TTCGAA TT^CGAA
BstF5I 470 CATCC GGATG(2/0)
DraII 95 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 461 CCATGG C^C[GA][CT]GG
EcoO109I 95 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 461 CCATGG C^C[AT][AT]GG
FokI 470 CATCC GGATG(9/13)
Hin1I 241 GGCGTC G[GA]^CG[CT]C
KpnI 119 GGTACC GGTAC^C
MaeIII 298 GTCAC ^GT[ACGT]AC
MamI 481 GATAGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 74 TCCGGA T^CCGGA
MspA1I 129 CCGCTG C[AC]G^C[GT]G
MwoI 71 GCTTCCGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 230 GCCGGC GCC^GGC
NcoI 461 CCATGG C^CATGG
NgoMIV 230 GCCGGC G^CCGGC
NspV 247 TTCGAA TT^CGAA
PflFI 91 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 445 CCAACCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 212 GAGTC GAGTC(4/5)
PpuMI 95 AGGTCCT [GA]G^G[AT]CC[CT]
SfaNI 370 GATGC GCATC(5/9)
SfuI 247 TTCGAA TT^CGAA
StyI 461 CCATGG C^C[AT][AT]GG
TfiI 325 GATTC G^A[AT]TC
Tsp45I 298 GTCAC ^GT[GC]AC
TspRI 388 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 91 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 445 CCAACCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 23 CGCG CG^CG
85 CGCG
AluI 305 AGCT AG^CT
431 AGCT
AvaI 37 CCCGAG C^[CT]CG[GA]G
160 CCCGAG
BbvI 113 GCAGC GCAGC(8/12)
306 GCTGC
BmyI 157 GAGCCC G[AGT]GC[ACT]^C
403 GTGCAC
BseRI 163 GAGGAG GAGGAG(10/8)
166 GAGGAG
BsoBI 37 CCCGAG C^[CT]CG[GA]G
160 CCCGAG
Bsp1286I 157 GAGCCC G[AGT]GC[ACT]^C
403 GTGCAC
BsrFI 116 GCCGGT [GA]^CCGG[CT]
230 GCCGGC
BstNI 237 CCTGG CC^[AT]GG
258 CCAGG
BstUI 23 CGCG CG^CG
85 CGCG
Cfr10I 116 GCCGGT [GA]^CCGG[CT]
230 GCCGGC
CpoI 125 CGGACCG CG^G[AT]CCG
185 CGGTCCG
EcoRII 237 CCTGG ^CC[AT]GG
258 CCAGG
HaeIII 229 GGCC GG^CC
465 GGCC
HgaI 21 GACGC GACGC(5/10)
242 GCGTC
HinfI 212 GAGTC G^A[ACGT]TC
325 GATTC
MaeII 277 ACGT A^CGT
281 ACGT
MvaI 237 CCTGG CC^[AT]GG
258 CCAGG
MvnI 23 CGCG CG^CG
85 CGCG
NlaIII 153 CATG CATG^
462 CATG
RsrII 125 CGGACCG CG^G[AT]CCG
185 CGGTCCG
TaqI 248 TCGA T^CGA
365 TCGA
TseI 113 GCAGC G^C[AT]GC
306 GCTGC
TthHB8I 248 TCGA T^CGA
365 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AfaI AlwI AvaII BcnI
BpmI BsaJI BsiYI BslI BssKI
Cac8I Cfr13I DpnII FauI Fnu4HI
HapII HpaII ItaI MboI MboII
MnlI MspI NciI NdeII NlaIV
RsaI Sau3AI Sau96I ScrFI