Restriction map for
pF1KE0448
##################################
# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspHI AspI AvaI
AviII AvrII BalI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I EarI
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoT22I FbaI FokI FseI FspI
HgaI Hin1I HincII HindII HpaI
HphI KasI Ksp632I KspI MaeII
MaeIII MamI MboII MfeI MluI
MluNI MroI MscI MseI MslI
MunI NarI NciI NcoI NdeI
NheI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tru9I Tsp45I Tth111I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
BamHI 330 GGATCC G^GATCC
BfaI 310 CTAG C^TAG
BpmI 139 CTCCAG CTGGAG(16/14)
BsiEI 171 CGGCCG CG[GA][CT]^CG
BsmFI 43 GGGAC GGGAC(10/14)
BsrBI 19 GAGCGG CCGCTC(-3/-3)
BsrFI 146 GCCGGC [GA]^CCGG[CT]
BssHII 114 GCGCGC G^CGCGC
Cfr10I 146 GCCGGC [GA]^CCGG[CT]
DdeI 423 CTCAG C^T[ACGT]AG
DraII 43 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 49 CCGTGG C^C[GA][CT]GG
EaeI 171 CGGCCG [CT]^GGCC[GA]
EagI 171 CGGCCG C^GGCCG
EclXI 171 CGGCCG C^GGCCG
Eco52I 171 CGGCCG C^GGCCG
EcoO109I 43 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 124 GATATC GAT^ATC
FauI 41 GCGGG CCCGC(4/6)
HaeII 156 GGCGCT [GA]GCGC^[CT]
HapII 147 CCGG C^CGG
HindIII 361 AAGCTT A^AGCTT
HpaII 147 CCGG C^CGG
MaeI 310 CTAG C^TAG
MspA1I 326 CAGCGG C[AC]G^C[GT]G
MspI 147 CCGG C^CGG
NaeI 146 GCCGGC GCC^GGC
NgoMIV 146 GCCGGC G^CCGGC
NotI 170 GCGGCCGC GC^GGCCGC
NspI 150 GCATGT [GA]CATG^[CT]
PflMI 66 CCAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 43 GGGACCC [GA]G^G[AT]CC[CT]
SfaNI 283 GATGC GCATC(5/9)
Tsp509I 292 AATT ^AATT
Van91I 66 CCAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 33 GGTACC G^GTACC
62 GGTACC
AfaI 34 GTAC GT^AC
63 GTAC
AluI 213 AGCT AG^CT
362 AGCT
Asp718I 33 GGTACC G^GTACC
62 GGTACC
AvaII 44 GGACC G^G[AT]CC
445 GGACC
BbvI 286 GCAGC GCAGC(8/12)
325 GCAGC
BseRI 16 GAGGAG GAGGAG(10/8)
76 GAGGAG
BsrI 57 CCAGT ACTGG(1/-1)
129 CCAGT
BstYI 179 AGATCC [GA]^GATC[CT]
330 GGATCC
DpnII 180 GATC ^GATC
331 GATC
EcoT14I 71 CCTTGG C^C[AT][AT]GG
334 CCTTGG
KpnI 33 GGTACC GGTAC^C
62 GGTACC
MboI 180 GATC ^GATC
331 GATC
MflI 179 AGATCC [GA]^GATC[CT]
330 GGATCC
NdeII 180 GATC ^GATC
331 GATC
NlaIII 151 CATG CATG^
227 CATG
RsaI 34 GTAC GT^AC
63 GTAC
Sau3AI 180 GATC ^GATC
331 GATC
SmlI 184 CTCAAG C^T[CT][GA]AG
463 CTTGAG
StyI 71 CCTTGG C^C[AT][AT]GG
334 CCTTGG
TseI 286 GCAGC G^C[AT]GC
325 GCAGC
XhoII 179 AGATCC [GA]^GATC[CT]
330 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AccII AciI AlwI BanI BsaJI
BsiYI BslI BssKI BstNI BstUI
Cac8I CfoI Cfr13I EcoRII Fnu4HI
HaeIII HhaI HinP1I HinfI ItaI
MnlI MvaI MvnI MwoI NlaIV
Sau96I ScrFI TfiI TspRI