Restriction map for
pF1KB7838
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII Acc65I AccIII AclI AgeI
AhdI AocI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BbeI BbsI BbvCI
BciVI BclI BfaI BlnI BlpI
Bpu1102I BpuAI BsaBI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI MaeI MamI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI ScaI SexAI
SfoI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI StuI SwaI
Van91I XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 305 GTCTAC GT^[AC][GT]AC
AccII 1623 CGCG CG^CG
AcsI 1662 GAATTC [GA]^AATT[CT]
AflII 1414 CTTAAG C^TTAAG
Alw44I 436 GTGCAC G^TGCAC
AlwI 830 GATCC GGATC(4/5)
AlwNI 124 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 436 GTGCAC G^TGCAC
ApoI 1662 GAATTC [GA]^AATT[CT]
AspI 1399 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1174 CACGTG CAC^GTG
BcgI 1642 GCAGACCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1414 CTTAAG C^TTAAG
BglI 743 GCCCCATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1174 CACGTG [CT]AC^GT[GA]
BsaI 1541 GAGACC GGTCTC(1/5)
BsaWI 771 TCCGGT [AT]^CCGG[AT]
BspLU11I 1262 ACATGT A^CATGT
BsrBI 1647 CCGCTC CCGCTC(-3/-3)
BsrDI 558 GCAATG GCAATG(2/0)
BsrFI 1730 ACCGGC [GA]^CCGG[CT]
BstUI 1623 CGCG CG^CG
Cfr10I 1730 ACCGGC [GA]^CCGG[CT]
EcoNI 1410 CCTGCTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1662 GAATTC G^AATTC
EcoRV 864 GATATC GAT^ATC
HaeII 1590 GGCGCT [GA]GCGC^[CT]
KspI 1622 CCGCGG CCGC^GG
MseI 1415 TTAA T^TAA
MvnI 1623 CGCG CG^CG
PaeR7I 596 CTCGAG C^TCGAG
PflFI 1399 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1174 CACGTG CAC^GTG
PmlI 1174 CACGTG CAC^GTG
SacII 1622 CCGCGG CCGC^GG
SapI 1705 GCTCTTC GCTCTTC(1/4)
SbfI 1512 CCTGCAGG CCTGCA^GG
SfiI 742 GGCCCCATGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 1512 CCTGCAGG CCTGCA^GG
TfiI 88 GAATC G^A[AT]TC
Tru9I 1415 TTAA T^TAA
Tth111I 1399 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 596 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 495 GTAC GT^AC
1266 GTAC
AflIII 1175 ACGTGT A^C[GA][CT]GT
1262 ACATGT
ApaI 750 GGGCCC GGGCC^C
881 GGGCCC
AspHI 351 GAGCAC G[AT]GC[AT]^C
436 GTGCAC
BalI 314 TGGCCA TGG^CCA
677 TGGCCA
BglII 893 AGATCT A^GATCT
1301 AGATCT
BsgI 1118 CTGCAC GTGCAG(16/14)
1294 GTGCAG
BsiHKAI 351 GAGCAC G[AT]GC[AT]^C
436 GTGCAC
BsmBI 599 GAGACG CGTCTC(1/5)
1690 GAGACG
BsmI 209 GCATTC GAATGC(1/-1)
1803 GCATTC
EaeI 314 TGGCCA [CT]^GGCC[GA]
677 TGGCCA
EarI 1615 CTCTTC CTCTTC(1/4)
1706 CTCTTC
EcoT22I 953 ATGCAT ATGCA^T
1801 ATGCAT
Ksp632I 1615 CTCTTC CTCTTC(1/4)
1706 CTCTTC
MluNI 314 TGGCCA TGG^CCA
677 TGGCCA
MscI 314 TGGCCA TGG^CCA
677 TGGCCA
NsiI 953 ATGCAT ATGCA^T
1801 ATGCAT
PleI 13 GACTC GAGTC(4/5)
303 GAGTC
PstI 1513 CTGCAG CTGCA^G
1640 CTGCAG
PvuII 814 CAGCTG CAG^CTG
1128 CAGCTG
RsaI 495 GTAC GT^AC
1266 GTAC
SmaI 878 CCCGGG CCC^GGG
1371 CCCGGG
Tsp509I 520 AATT ^AATT
1663 AATT
XmaCI 878 CCCGGG C^CCGGG
1371 CCCGGG
XmaI 878 CCCGGG C^CCGGG
1371 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcyI AluI AvaI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaHI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XhoII